ID   NAHE2_PSEPU             Reviewed;         334 AA.
AC   P0A144; Q57444;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Trans-O-hydroxybenzylidenepyruvate hydratase-aldolase;
DE            Short=THBPA hydratase-aldolase;
DE            EC=4.1.2.45;
DE   AltName: Full=2'-hydroxybenzalpyruvate aldolase;
GN   Name=nahE;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid NPL1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BS202;
RA   Bezborodnikov S.G., Boronin A.M., Tiedje J.M.;
RT   "Nucleotide sequences of genes encoding an upper pathway of naphthalene
RT   metabolism of NPL1 plasmid from Pseudomonas putida strain BS202.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the naphthalene upper catabolic pathway.
CC       Catalyzes the transformation of trans-O-hydroxybenzylidenepyruvate
CC       (THBPA) to salicylaldehyde and pyruvate. The reaction is reversible (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = pyruvate +
CC         salicylaldehyde; Xref=Rhea:RHEA:27389, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:16008, ChEBI:CHEBI:59353; EC=4.1.2.45;
CC   -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC   -!- SIMILARITY: Belongs to the DapA family. {ECO:0000305}.
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DR   EMBL; AF010471; AAB62713.1; -; Genomic_DNA.
DR   RefSeq; NP_863078.1; NC_004999.1.
DR   RefSeq; WP_011117406.1; NC_004999.1.
DR   AlphaFoldDB; P0A144; -.
DR   SMR; P0A144; -.
DR   BioCyc; MetaCyc:MON-12809; -.
DR   UniPathway; UPA00082; -.
DR   GO; GO:0018813; F:trans-o-hydroxybenzylidenepyruvate hydratase-aldolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   PANTHER; PTHR12128; PTHR12128; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   PRINTS; PR00146; DHPICSNTHASE.
DR   SMART; SM01130; DHDPS; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase; Plasmid; Pyruvate.
FT   CHAIN           1..334
FT                   /note="Trans-O-hydroxybenzylidenepyruvate hydratase-
FT                   aldolase"
FT                   /id="PRO_0000096703"
SQ   SEQUENCE   334 AA;  36942 MW;  D32E7909EE678EA6 CRC64;
     MSNKIMKTSR LTAEDINGAW TIMPTPSTPD ASDWRSTATV DLEETARIVE ELIAAGVNGI
     LSMGTFGECA TLTWDEKRDY VSTIVETIRG RVPYFCGTTA LNTREVIRQT RELIDIGANG
     TMLGVPMWVK MDLPTAVQFY RDVADAVPEA AIAIYANPEA FKFDFPRPFW AEMSKIPQVV
     TAKYLGIGML DLDLRLAPNI RFLPHEDDYY AAARINPERI TAFWSSGAMC GPATAIMLRD
     EVVRAKSTGD WAKAKAISDD MRAADSTLFP RGDFSEFSKY NIGLEKARMD AAGWLKAGPC
     RPPYNLVPED YLAGAQKSGK AWAALHAKYS NELK