ID   NAHE_PSEAI              Reviewed;         334 AA.
AC   P0A142; Q57444;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Trans-O-hydroxybenzylidenepyruvate hydratase-aldolase;
DE            Short=THBPA hydratase-aldolase;
DE            EC=4.1.2.45;
DE   AltName: Full=2'-hydroxybenzalpyruvate aldolase;
GN   Name=pahE;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PaK1;
RA   Takizawa N., Iida T., Yamauchi K., Satoh S., Wang Y., Fukuda M.,
RA   Kiyohara H.;
RT   "The molecular analysis of an NAH7-type gene cluster, pah, located on the
RT   chromosome of Pseudomonas aeruginosa PaK1.";
RL   Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the naphthalene upper catabolic pathway.
CC       Catalyzes the transformation of trans-O-hydroxybenzylidenepyruvate
CC       (THBPA) to salicylaldehyde and pyruvate. The reaction is reversible (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = pyruvate +
CC         salicylaldehyde; Xref=Rhea:RHEA:27389, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:16008, ChEBI:CHEBI:59353; EC=4.1.2.45;
CC   -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC   -!- SIMILARITY: Belongs to the DapA family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D84146; BAA12246.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0A142; -.
DR   SMR; P0A142; -.
DR   UniPathway; UPA00082; -.
DR   GO; GO:0018813; F:trans-o-hydroxybenzylidenepyruvate hydratase-aldolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   PANTHER; PTHR12128; PTHR12128; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   PRINTS; PR00146; DHPICSNTHASE.
DR   SMART; SM01130; DHDPS; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase; Pyruvate.
FT   CHAIN           1..334
FT                   /note="Trans-O-hydroxybenzylidenepyruvate hydratase-
FT                   aldolase"
FT                   /id="PRO_0000096704"
SQ   SEQUENCE   334 AA;  36942 MW;  D32E7909EE678EA6 CRC64;
     MSNKIMKTSR LTAEDINGAW TIMPTPSTPD ASDWRSTATV DLEETARIVE ELIAAGVNGI
     LSMGTFGECA TLTWDEKRDY VSTIVETIRG RVPYFCGTTA LNTREVIRQT RELIDIGANG
     TMLGVPMWVK MDLPTAVQFY RDVADAVPEA AIAIYANPEA FKFDFPRPFW AEMSKIPQVV
     TAKYLGIGML DLDLRLAPNI RFLPHEDDYY AAARINPERI TAFWSSGAMC GPATAIMLRD
     EVVRAKSTGD WAKAKAISDD MRAADSTLFP RGDFSEFSKY NIGLEKARMD AAGWLKAGPC
     RPPYNLVPED YLAGAQKSGK AWAALHAKYS NELK