ID   NAHE_PSEU8              Reviewed;         334 AA.
AC   P0A143; Q57444;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Trans-O-hydroxybenzylidenepyruvate hydratase-aldolase;
DE            Short=THBPA hydratase-aldolase;
DE            EC=4.1.2.45;
DE   AltName: Full=2'-hydroxybenzalpyruvate aldolase;
GN   Name=doxI;
OS   Pseudomonas sp. (strain C18).
OG   Plasmid unnamed.
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=69011;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=C18; PLASMID=unnamed;
RX   PubMed=8226631; DOI=10.1128/jb.175.21.6890-6901.1993;
RA   Denome S.A., Stanley D.C., Olson E.S., Young K.D.;
RT   "Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains:
RT   complete DNA sequence of an upper naphthalene catabolic pathway.";
RL   J. Bacteriol. 175:6890-6901(1993).
CC   -!- FUNCTION: Involved in the naphthalene upper catabolic pathway.
CC       Catalyzes the transformation of trans-O-hydroxybenzylidenepyruvate
CC       (THBPA) to salicylaldehyde and pyruvate. The reaction is reversible
CC       (Probable). {ECO:0000305|PubMed:8226631}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = pyruvate +
CC         salicylaldehyde; Xref=Rhea:RHEA:27389, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:16008, ChEBI:CHEBI:59353; EC=4.1.2.45;
CC   -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC   -!- MISCELLANEOUS: Encoded on an unnamed 75 kb plasmid.
CC       {ECO:0000305|PubMed:8226631}.
CC   -!- SIMILARITY: Belongs to the DapA family. {ECO:0000305}.
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DR   EMBL; M60405; AAA16132.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0A143; -.
DR   SMR; P0A143; -.
DR   UniPathway; UPA00082; -.
DR   GO; GO:0018813; F:trans-o-hydroxybenzylidenepyruvate hydratase-aldolase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   PANTHER; PTHR12128; PTHR12128; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   PRINTS; PR00146; DHPICSNTHASE.
DR   SMART; SM01130; DHDPS; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Lyase; Plasmid; Pyruvate.
FT   CHAIN           1..334
FT                   /note="Trans-O-hydroxybenzylidenepyruvate hydratase-
FT                   aldolase"
FT                   /id="PRO_0000096705"
SQ   SEQUENCE   334 AA;  36942 MW;  D32E7909EE678EA6 CRC64;
     MSNKIMKTSR LTAEDINGAW TIMPTPSTPD ASDWRSTATV DLEETARIVE ELIAAGVNGI
     LSMGTFGECA TLTWDEKRDY VSTIVETIRG RVPYFCGTTA LNTREVIRQT RELIDIGANG
     TMLGVPMWVK MDLPTAVQFY RDVADAVPEA AIAIYANPEA FKFDFPRPFW AEMSKIPQVV
     TAKYLGIGML DLDLRLAPNI RFLPHEDDYY AAARINPERI TAFWSSGAMC GPATAIMLRD
     EVVRAKSTGD WAKAKAISDD MRAADSTLFP RGDFSEFSKY NIGLEKARMD AAGWLKAGPC
     RPPYNLVPED YLAGAQKSGK AWAALHAKYS NELK