NAHF_PSEPU
ID NAHF_PSEPU Reviewed; 483 AA.
AC P0A391; O34269; Q52460;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=Salicylaldehyde dehydrogenase;
DE EC=1.2.1.65;
GN Name=nahF;
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OG Plasmid NPL1, and Plasmid NPL-41.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BS202; PLASMID=NPL1;
RA Bezborodnikov S.G., Boronin A.M., Tiedje J.M.;
RT "Nucleotide sequences of genes encoding an upper pathway of naphthalene
RT metabolism of NPL1 plasmid from Pseudomonas putida strain BS202.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 221-483.
RC STRAIN=BS202; PLASMID=NPL-41;
RA Piruzian E.S., Serebryiskaya T.S., Lenets A.A., Goldenkova I.V.,
RA Kobets N.S.;
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) + salicylaldehyde = 2 H(+) + NADH + salicylate;
CC Xref=Rhea:RHEA:18537, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16008, ChEBI:CHEBI:30762, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.2.1.65;
CC -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC {ECO:0000305}.
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DR EMBL; AF010471; AAB62710.1; -; Genomic_DNA.
DR EMBL; Y14173; CAA74578.1; -; Genomic_DNA.
DR RefSeq; NP_863075.1; NC_004999.1.
DR RefSeq; WP_011117403.1; NC_004999.1.
DR AlphaFoldDB; P0A391; -.
DR SMR; P0A391; -.
DR BioCyc; MetaCyc:MON-12810; -.
DR UniPathway; UPA00082; -.
DR GO; GO:0018485; F:salicylaldehyde dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.309.10; -; 1.
DR Gene3D; 3.40.605.10; -; 1.
DR InterPro; IPR016161; Ald_DH/histidinol_DH.
DR InterPro; IPR016163; Ald_DH_C.
DR InterPro; IPR029510; Ald_DH_CS_GLU.
DR InterPro; IPR016162; Ald_DH_N.
DR InterPro; IPR015590; Aldehyde_DH_dom.
DR Pfam; PF00171; Aldedh; 1.
DR SUPFAM; SSF53720; SSF53720; 1.
DR PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE 3: Inferred from homology;
KW Aromatic hydrocarbons catabolism; NAD; Oxidoreductase; Plasmid.
FT CHAIN 1..483
FT /note="Salicylaldehyde dehydrogenase"
FT /id="PRO_0000056589"
FT ACT_SITE 250
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT ACT_SITE 284
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT BINDING 228..233
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT CONFLICT 318
FT /note="V -> RL (in Ref. 2; CAA74578)"
FT /evidence="ECO:0000305"
FT CONFLICT 365
FT /note="I -> M (in Ref. 2; CAA74578)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 483 AA; 51994 MW; CD606BADD21DFACE CRC64;
MKTKLFINNA WIDSSDQQTF ERIHPVSSDV VTESANATVT DAIKAAQAAE EAFKTWKAVG
PSERRRLLLK VADVMESKTP KFIEVMAMEV GASALWAGFN VHASANVFRE AASLATQIQG
ETIPTDKAET LSMTLRQPVG PILSIVPWNG TAVLAARAIA YPLVCGNTVV FKGSEFSPAT
HALITQCVQE AGLPAGVLNY LNSSPDRSPE IADALISAKE IRRINFTGST RVGSIIAQKA
AQHLKRCLLE LGGKSPLIVL DDADIDAAVK AAVFGSFLFQ GQICMSTERL IVDEKIADEF
VAKFVEKTKR LSAGDPCVTG DCIIGPMVSP NSGERINGLF KDAIDKGAKV VCGGLAQGAL
MPATILDHVK SDMRIYDEET FGPITVVIRC KGEAEAVRIA NDSVYGLSSG VFGRDINRAL
RVGMSIEYGS VHINGSTVQN EAQAPYGGTK NTGYGRFDGR AVIDEFTEIK WLTIEPFEQQ
YPF