ID   NAHF_PSEU8              Reviewed;         483 AA.
AC   P0A390; O34269; Q52460;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=Salicylaldehyde dehydrogenase;
DE            EC=1.2.1.65;
GN   Name=doxF;
OS   Pseudomonas sp. (strain C18).
OG   Plasmid unnamed.
OC   Bacteria; Proteobacteria.
OX   NCBI_TaxID=69011;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C18; PLASMID=unnamed;
RX   PubMed=8226631; DOI=10.1128/jb.175.21.6890-6901.1993;
RA   Denome S.A., Stanley D.C., Olson E.S., Young K.D.;
RT   "Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains:
RT   complete DNA sequence of an upper naphthalene catabolic pathway.";
RL   J. Bacteriol. 175:6890-6901(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + NAD(+) + salicylaldehyde = 2 H(+) + NADH + salicylate;
CC         Xref=Rhea:RHEA:18537, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16008, ChEBI:CHEBI:30762, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.65;
CC   -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC   -!- MISCELLANEOUS: Encoded on an unnamed 75 kb plasmid.
CC       {ECO:0000305|PubMed:8226631}.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; M60405; AAA16129.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0A390; -.
DR   SMR; P0A390; -.
DR   UniPathway; UPA00082; -.
DR   GO; GO:0018485; F:salicylaldehyde dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   Pfam; PF00171; Aldedh; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase; Plasmid.
FT   CHAIN           1..483
FT                   /note="Salicylaldehyde dehydrogenase"
FT                   /id="PRO_0000056590"
FT   ACT_SITE        250
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT   ACT_SITE        284
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT   BINDING         228..233
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   483 AA;  51994 MW;  CD606BADD21DFACE CRC64;
     MKTKLFINNA WIDSSDQQTF ERIHPVSSDV VTESANATVT DAIKAAQAAE EAFKTWKAVG
     PSERRRLLLK VADVMESKTP KFIEVMAMEV GASALWAGFN VHASANVFRE AASLATQIQG
     ETIPTDKAET LSMTLRQPVG PILSIVPWNG TAVLAARAIA YPLVCGNTVV FKGSEFSPAT
     HALITQCVQE AGLPAGVLNY LNSSPDRSPE IADALISAKE IRRINFTGST RVGSIIAQKA
     AQHLKRCLLE LGGKSPLIVL DDADIDAAVK AAVFGSFLFQ GQICMSTERL IVDEKIADEF
     VAKFVEKTKR LSAGDPCVTG DCIIGPMVSP NSGERINGLF KDAIDKGAKV VCGGLAQGAL
     MPATILDHVK SDMRIYDEET FGPITVVIRC KGEAEAVRIA NDSVYGLSSG VFGRDINRAL
     RVGMSIEYGS VHINGSTVQN EAQAPYGGTK NTGYGRFDGR AVIDEFTEIK WLTIEPFEQQ
     YPF