ID   NAHH_PSEPU              Reviewed;         307 AA.
AC   P08127;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Metapyrocatechase;
DE            Short=MPC;
DE            EC=1.13.11.2;
DE   AltName: Full=CatO2ase;
DE   AltName: Full=Catechol 2,3-dioxygenase;
GN   Name=nahH;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid NAH7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 17485 / DSM 50208 / JCM 6158 / NCIMB 12092 / Stanier 111 /
RC   Biotype A;
RX   PubMed=3481421; DOI=10.1007/bf00325689;
RA   Harayama S., Rekik M., Wasserfallen A., Bairoch A.;
RT   "Evolutionary relationships between catabolic pathways for aromatics:
RT   conservation of gene order and nucleotide sequences of catechol oxidation
RT   genes of pWW0 and NAH7 plasmids.";
RL   Mol. Gen. Genet. 210:241-247(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 17485 / DSM 50208 / JCM 6158 / NCIMB 12092 / Stanier 111 /
RC   Biotype A;
RX   PubMed=3623105; DOI=10.1016/0378-1119(87)90244-7;
RA   Ghosal D., You I.-S., Gunsalus I.C.;
RT   "Nucleotide sequence and expression of gene nahH of plasmid NAH7 and
RT   homology with gene xylE of TOL pWWO.";
RL   Gene 55:19-28(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
RC   STRAIN=ATCC 17485 / DSM 50208 / JCM 6158 / NCIMB 12092 / Stanier 111 /
RC   Biotype A;
RX   PubMed=1993181; DOI=10.1021/bi00220a028;
RA   You I.-S., Ghosal D., Gunsalus I.C.;
RT   "Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate
RT   hydroxylase gene (nahG) and its 3'-flanking region.";
RL   Biochemistry 30:1635-1641(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=catechol + O2 = (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate +
CC         H(+); Xref=Rhea:RHEA:17337, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:18135, ChEBI:CHEBI:71198; EC=1.13.11.2;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC   -!- PATHWAY: Aromatic compound metabolism; benzoate degradation via
CC       hydroxylation.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the extradiol ring-cleavage dioxygenase family.
CC       {ECO:0000305}.
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DR   EMBL; M17159; AAA98183.1; -; Genomic_DNA.
DR   EMBL; X06412; CAA29701.1; -; Genomic_DNA.
DR   EMBL; M60055; AAA25899.1; -; Genomic_DNA.
DR   PIR; A27389; A27389.
DR   RefSeq; WP_011475388.1; NC_007926.1.
DR   RefSeq; YP_534833.1; NC_007926.1.
DR   AlphaFoldDB; P08127; -.
DR   SMR; P08127; -.
DR   UniPathway; UPA00156; -.
DR   GO; GO:0018577; F:catechol 2,3-dioxygenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0043640; P:benzoate catabolic process via hydroxylation; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.10.180.10; -; 2.
DR   InterPro; IPR017624; Catechol_2-3_dOase.
DR   InterPro; IPR029068; Glyas_Bleomycin-R_OHBP_Dase.
DR   InterPro; IPR004360; Glyas_Fos-R_dOase_dom.
DR   InterPro; IPR037523; VOC.
DR   InterPro; IPR000486; Xdiol_ring_cleave_dOase_1/2.
DR   Pfam; PF00903; Glyoxalase; 1.
DR   SUPFAM; SSF54593; SSF54593; 1.
DR   TIGRFAMs; TIGR03211; catechol_2_3; 1.
DR   PROSITE; PS00082; EXTRADIOL_DIOXYGENAS; 1.
DR   PROSITE; PS51819; VOC; 2.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Iron; Metal-binding;
KW   Oxidoreductase; Plasmid; Repeat.
FT   CHAIN           1..307
FT                   /note="Metapyrocatechase"
FT                   /id="PRO_0000085024"
FT   DOMAIN          7..122
FT                   /note="VOC 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT   DOMAIN          150..269
FT                   /note="VOC 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01163"
FT   BINDING         153
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         214
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
FT   BINDING         265
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   307 AA;  34913 MW;  140465CAA0CE7F4E CRC64;
     MNKGVMRPGH VQLRVLDMGK ALEHYVELLG LIEMDRDDQG RVYLKAWTEV DKFSVVLREA
     DEPGMDFMGF KVVDEDSLNR LTDDLLNFGC LIENVAAGEL KGCGRRVRFQ APSGHHFELY
     ADKEYTGKWG VSEVNPEAWP RDLKGMAAVR FDHCLLYGDE LQATYELFTE VLGFYLAEQV
     VDADGIRLAQ FLSLSTKAHD VAFIHHAEKG KFHHASFFLD TWEDVLRAAD LISMTDTSID
     IGPTRHGLTQ GKTIYFFDPS GNRCEVFCGG NYNYPDHKPV TWLAKDVGKA IFYHDRVLNE
     RFMTVMT