ID   NAHY_PSEPU              Reviewed;         538 AA.
AC   Q9Z429;
DT   22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Methyl-accepting chemotaxis protein NahY;
GN   Name=nahY;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid NAH7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 17485 / DSM 50208 / JCM 6158 / NCIMB 12092 / Stanier 111 /
RC   Biotype A; PLASMID=NAH7;
RX   PubMed=10322041; DOI=10.1128/jb.181.10.3310-3316.1999;
RA   Grimm A.C., Harwood C.S.;
RT   "NahY, a catabolic plasmid-encoded receptor required for chemotaxis of
RT   Pseudomonas putida to the aromatic hydrocarbon naphthalene.";
RL   J. Bacteriol. 181:3310-3316(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 17485 / DSM 50208 / JCM 6158 / NCIMB 12092 / Stanier 111 /
RC   Biotype A; PLASMID=NAH7;
RX   PubMed=16707697; DOI=10.1128/jb.00185-06;
RA   Sota M., Yano H., Ono A., Miyazaki R., Ishii H., Genka H., Top E.M.,
RA   Tsuda M.;
RT   "Genomic and functional analysis of the IncP-9 naphthalene-catabolic
RT   plasmid NAH7 and its transposon Tn4655 suggests catabolic gene spread by a
RT   tyrosine recombinase.";
RL   J. Bacteriol. 188:4057-4067(2006).
CC   -!- FUNCTION: Chemotactic-signal transducers respond to changes in the
CC       concentration of attractants and repellents in the environment,
CC       transduce a signal from the outside to the inside of the cell, and
CC       facilitate sensory adaptation through the variation of the level of
CC       methylation. Chemoreceptor for naphthalene or a related compound. May
CC       facilitate biodegradation. {ECO:0000269|PubMed:10322041}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Mutant grows on naphthalene but is not
CC       chemotactic to this aromatic hydrocarbon. Mutant has a wild-type
CC       chemotactic response to succinate, benzoate, salicylate and 4-
CC       hydroxybenzoate. {ECO:0000269|PubMed:10322041}.
CC   -!- SIMILARITY: Belongs to the methyl-accepting chemotaxis (MCP) protein
CC       family. {ECO:0000305}.
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DR   EMBL; AF100302; AAD13223.1; -; Genomic_DNA.
DR   EMBL; AB237655; BAE92176.1; -; Genomic_DNA.
DR   RefSeq; WP_011475397.1; NC_007926.1.
DR   RefSeq; YP_534842.1; NC_007926.1.
DR   AlphaFoldDB; Q9Z429; -.
DR   SMR; Q9Z429; -.
DR   BindingDB; Q9Z429; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004888; F:transmembrane signaling receptor activity; IEA:InterPro.
DR   GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
DR   GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR   InterPro; IPR004090; Chemotax_Me-accpt_rcpt.
DR   InterPro; IPR003660; HAMP_dom.
DR   InterPro; IPR024478; HlyB_4HB_MCP.
DR   InterPro; IPR004089; MCPsignal_dom.
DR   InterPro; IPR000727; T_SNARE_dom.
DR   Pfam; PF12729; 4HB_MCP_1; 1.
DR   Pfam; PF00672; HAMP; 1.
DR   Pfam; PF00015; MCPsignal; 1.
DR   PRINTS; PR00260; CHEMTRNSDUCR.
DR   SMART; SM00304; HAMP; 1.
DR   SMART; SM00283; MA; 1.
DR   PROSITE; PS50111; CHEMOTAXIS_TRANSDUC_2; 1.
DR   PROSITE; PS50885; HAMP; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Chemotaxis; Membrane; Methylation;
KW   Plasmid; Transducer; Transmembrane; Transmembrane helix.
FT   CHAIN           1..538
FT                   /note="Methyl-accepting chemotaxis protein NahY"
FT                   /id="PRO_0000424881"
FT   TOPO_DOM        1..9
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        31..187
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        188..208
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        209..538
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          209..261
FT                   /note="HAMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00102"
FT   DOMAIN          266..502
FT                   /note="Methyl-accepting transducer"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00284"
SQ   SEQUENCE   538 AA;  58716 MW;  C91589BE65F45E56 CRC64;
     MQQFTIRTRL LMLVGAMFIG FITIELMGFS ALQRGVASLN TVYLDRVVPL RDLKTIADLY
     AVKIVDSSHK ARSGRMTYAQ AEQEVKDAGR QIDMLWHAYQ KTKKIDEEQR SVDALAKLVD
     EAQDPIERLK GILERGDKAA LDTFVENEMY PLIDPLSEGL SHLTQIQVEE SKRAYDAAVV
     LYDSSRTMLA LLLLGILICG GVFATRLIRS IIHPLTTLKD AAARVALGDL SQSIQVSGRN
     EVTDVQQSVQ AMQANLRNTL QDIQGSAAQL AAAAEELQTA TESTAQGIHR QNDEMQMAAT
     AVTEMSAAVD EVADNANRTS NASHEAMDLA DGGRKQVMLT RETIDRLSGK LNETTRTVFR
     LAEEASNIGR VLDVIRAIAE QTKLLALNAA IEAAHAGEAG RGFAVVADEV RNLAQRTQTS
     TQEIERMISA IQSVTQEGVR DVQQSCEFAA RSQTMSSEAD QALTLIAERI TEINGMNLVI
     ASAAEEQAQV AREVDRNLVA ISDISEQSTA GVQQTSEASE ELARLAANLN NLVNRFSM