ID   NAKD2_XENTR             Reviewed;         427 AA.
AC   Q08CZ6;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=NAD kinase 2, mitochondrial;
DE            EC=2.7.1.23;
DE   AltName: Full=NAD kinase domain-containing protein 1, mitochondrial;
DE   Flags: Precursor;
GN   Name=nadk2; Synonyms=nadkd1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=N6; TISSUE=Skeletal muscle;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to
CC       yield NADP(+). Can use both ATP or inorganic polyphosphate as the
CC       phosphoryl donor (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + NAD(+) = ADP + H(+) + NADP(+); Xref=Rhea:RHEA:18629,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:456216; EC=2.7.1.23;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NAD kinase family. {ECO:0000305}.
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DR   EMBL; BC124020; AAI24021.1; -; mRNA.
DR   RefSeq; NP_001072687.1; NM_001079219.1.
DR   AlphaFoldDB; Q08CZ6; -.
DR   PaxDb; Q08CZ6; -.
DR   Ensembl; ENSXETT00000010405; ENSXETP00000010405; ENSXETG00000004798.
DR   GeneID; 780144; -.
DR   KEGG; xtr:780144; -.
DR   CTD; 133686; -.
DR   Xenbase; XB-GENE-991224; nadk2.
DR   eggNOG; KOG4180; Eukaryota.
DR   HOGENOM; CLU_039559_0_0_1; -.
DR   InParanoid; Q08CZ6; -.
DR   OMA; QGSEGYM; -.
DR   OrthoDB; 1416565at2759; -.
DR   PhylomeDB; Q08CZ6; -.
DR   TreeFam; TF314077; -.
DR   Reactome; R-XTR-196807; Nicotinate metabolism.
DR   Proteomes; UP000008143; Chromosome 1.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000004798; Expressed in mesonephros and 14 other tissues.
DR   ExpressionAtlas; Q08CZ6; baseline.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003951; F:NAD+ kinase activity; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0019674; P:NAD metabolic process; ISS:UniProtKB.
DR   GO; GO:0006741; P:NADP biosynthetic process; IEA:InterPro.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.200.30; -; 1.
DR   Gene3D; 3.40.50.10330; -; 1.
DR   InterPro; IPR017438; ATP-NAD_kinase_N.
DR   InterPro; IPR017437; ATP-NAD_kinase_PpnK-typ_C.
DR   InterPro; IPR016064; NAD/diacylglycerol_kinase_sf.
DR   InterPro; IPR002504; NADK.
DR   InterPro; IPR012355; NADK2_mit.
DR   Pfam; PF01513; NAD_kinase; 1.
DR   PIRSF; PIRSF017565; Kin_ATP-NAD_euk; 1.
DR   SUPFAM; SSF111331; SSF111331; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Kinase; Mitochondrion; NAD; NADP; Nucleotide-binding;
KW   Reference proteome; Transferase; Transit peptide.
FT   TRANSIT         1..33
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           34..427
FT                   /note="NAD kinase 2, mitochondrial"
FT                   /id="PRO_0000296295"
SQ   SEQUENCE   427 AA;  48050 MW;  A70369303A94E1F7 CRC64;
     MSLCLRLLCS VCGAAALRVP LGVSSLRALS GSAEGFRPAR VAVVAKTTRY EFEQQRYRSS
     GLSEAELRDL LALKGSSYNG LLQRYNIHSE NVEHIVQSLR KEGTDVRLVK RRDYDEETVR
     WADAIISAGG DGTMLLAASK VQDRFKPVIG VNTDPERSEG HLCLPVRYTW SFPEALQKLY
     RGEFRWQWRQ RIRLYLEGTG INLTPVDLHE QQLSLEQHNK AHNSQLEQKS VAVSGPQLLP
     VRALNEVFIG ESLSSRVNYK SCKPRFTFSL HRASYYEISV DDGPWEKQKS SGLNVCTGTG
     SKAWSYNINK MSSQSVEELL NIVRQHKSLN VSLDSDVIQR VTNAYNDSLV YNPEEPKMFF
     SVREPIANRV FSSSQQRGFT SKVCVRSRCW DACMVVDGGT SFEFNDGAIV SIVMDDQDAL
     CTVLLDD