NAKR3_ARATH
ID NAKR3_ARATH Reviewed; 247 AA.
AC Q8RXH8; F4JAG5; Q9LFG6;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 {ECO:0000303|PubMed:21193571};
DE Short=NaKR3 {ECO:0000303|PubMed:21193571};
DE AltName: Full=Heavy metal-associated plant protein 4 {ECO:0000303|PubMed:23368984};
DE Short=AtHPP04 {ECO:0000303|PubMed:23368984};
GN Name=NAKR3 {ECO:0000303|PubMed:21193571};
GN Synonyms=HPP04 {ECO:0000303|PubMed:23368984};
GN OrderedLocusNames=At3g53530 {ECO:0000312|Araport:AT3G53530};
GN ORFNames=F4P12.230 {ECO:0000312|EMBL:CAB67660.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAL87355.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=21193571; DOI=10.1105/tpc.110.080010;
RA Tian H., Baxter I.R., Lahner B., Reinders A., Salt D.E., Ward J.M.;
RT "Arabidopsis NPCC6/NaKR1 is a phloem mobile metal binding protein necessary
RT for phloem function and root meristem maintenance.";
RL Plant Cell 22:3963-3979(2010).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=23368984; DOI=10.1111/febs.12159;
RA de Abreu-Neto J.B., Turchetto-Zolet A.C., de Oliveira L.F., Zanettini M.H.,
RA Margis-Pinheiro M.;
RT "Heavy metal-associated isoprenylated plant protein (HIPP):
RT characterization of a family of proteins exclusive to plants.";
RL FEBS J. 280:1604-1616(2013).
RN [6]
RP FUNCTION, INDUCTION BY SALT AND OSMOTIC STRESS, AND SUBCELLULAR LOCATION.
RX PubMed=26909945; DOI=10.4238/gmr.15016378;
RA Luo Q., Zhao Z., Li D.K., Zhang Y., Xie L.F., Peng M.F., Yuan S., Yang Y.;
RT "Overexpression of NaKR3 enhances salt tolerance in Arabidopsis.";
RL Genet. Mol. Res. 15:0-0(2016).
CC -!- FUNCTION: Heavy metal-associated protein involved in salt tolerance.
CC {ECO:0000269|PubMed:26909945}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:26909945}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8RXH8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8RXH8-2; Sequence=VSP_058511;
CC -!- INDUCTION: Up-regulated by salt and osmotic stress.
CC {ECO:0000269|PubMed:26909945}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB67660.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL132966; CAB67660.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE79104.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79105.1; -; Genomic_DNA.
DR EMBL; AY080885; AAL87355.1; -; mRNA.
DR EMBL; AY113972; AAM45020.1; -; mRNA.
DR PIR; T45893; T45893.
DR RefSeq; NP_001030852.1; NM_001035775.2. [Q8RXH8-2]
DR RefSeq; NP_190921.2; NM_115213.4. [Q8RXH8-1]
DR AlphaFoldDB; Q8RXH8; -.
DR SMR; Q8RXH8; -.
DR STRING; 3702.AT3G53530.2; -.
DR iPTMnet; Q8RXH8; -.
DR PRIDE; Q8RXH8; -.
DR ProteomicsDB; 251044; -. [Q8RXH8-1]
DR EnsemblPlants; AT3G53530.1; AT3G53530.1; AT3G53530. [Q8RXH8-1]
DR EnsemblPlants; AT3G53530.2; AT3G53530.2; AT3G53530. [Q8RXH8-2]
DR GeneID; 824521; -.
DR Gramene; AT3G53530.1; AT3G53530.1; AT3G53530. [Q8RXH8-1]
DR Gramene; AT3G53530.2; AT3G53530.2; AT3G53530. [Q8RXH8-2]
DR KEGG; ath:AT3G53530; -.
DR Araport; AT3G53530; -.
DR TAIR; locus:2083966; AT3G53530.
DR eggNOG; KOG1603; Eukaryota.
DR HOGENOM; CLU_071922_0_1_1; -.
DR OMA; KSIGCFD; -.
DR OrthoDB; 1257261at2759; -.
DR PhylomeDB; Q8RXH8; -.
DR PRO; PR:Q8RXH8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8RXH8; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd00371; HMA; 1.
DR InterPro; IPR006121; HMA_dom.
DR InterPro; IPR036163; HMA_dom_sf.
DR InterPro; IPR044526; NAKR1/2/3.
DR InterPro; IPR016578; NAKR2/3.
DR PANTHER; PTHR46119; PTHR46119; 1.
DR Pfam; PF00403; HMA; 1.
DR PIRSF; PIRSF011221; Chloropl_CC_prd; 1.
DR SUPFAM; SSF55008; SSF55008; 1.
DR PROSITE; PS50846; HMA_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Metal-binding; Reference proteome.
FT CHAIN 1..247
FT /note="Protein SODIUM POTASSIUM ROOT DEFECTIVE 3"
FT /id="PRO_0000437275"
FT DOMAIN 167..235
FT /note="HMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT REGION 130..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 180
FT /ligand="a metal cation"
FT /ligand_id="ChEBI:CHEBI:25213"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT BINDING 183
FT /ligand="a metal cation"
FT /ligand_id="ChEBI:CHEBI:25213"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT VAR_SEQ 195
FT /note="Q -> QV (in isoform 2)"
FT /id="VSP_058511"
SQ SEQUENCE 247 AA; 26153 MW; E38A40EC2371859B CRC64;
MKAGMFYCAS QASTATANGE RTVTARAIDR HNPIIKDGRR SFTAPCSSGD DYVAPYRQLS
KITRVPSSSG DGKSVQVDKG RRSNSGSLMK LISYDVSLAR KSFGCVVATP KTPPGSTRYL
LGSDPVSLAG STGQDTVATE ESEASAPKRG SSGPVEEKKK SSGSGSDQVV VLRVSLHCHC
RGCQGKVKKH LSKMQGVTSF NIDFASKKVT VTGDITPLEV LGCLSKVKNA QFWTPPPPSI
PRANPET
