NAL1_ORYSJ
ID NAL1_ORYSJ Reviewed; 582 AA.
AC B4XT64; A0A0P0WF43; Q0JA57;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 25-MAY-2022, entry version 60.
DE RecName: Full=Protein NARROW LEAF 1 {ECO:0000303|PubMed:18562767};
DE AltName: Full=Protein GREEN FOR PHOTOSYNTHESIS {ECO:0000303|PubMed:23985993};
DE AltName: Full=Protein QUANTITATIVE TRAIT LOCUS FOR FLAG LEAF WIDTH 4 {ECO:0000305};
DE Short=qFLW4 {ECO:0000303|PubMed:22179305};
DE AltName: Full=Protein SPIKELET NUMBER {ECO:0000303|PubMed:24297875};
GN Name=NAL1 {ECO:0000303|PubMed:18562767};
GN Synonyms=GFP {ECO:0000303|PubMed:23985993},
GN LSCHL4 {ECO:0000303|PubMed:24795339}, SPIKE {ECO:0000303|PubMed:24297875};
GN OrderedLocusNames=Os04g0615000 {ECO:0000312|EMBL:BAF15780.1},
GN LOC_Os04g52479 {ECO:0000305};
GN ORFNames=OsJ_16147 {ECO:0000312|EMBL:EEE61678.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=18562767; DOI=10.1104/pp.108.118778;
RA Qi J., Qian Q., Bu Q., Li S., Chen Q., Sun J., Liang W., Zhou Y., Chu C.,
RA Li X., Ren F., Palme K., Zhao B., Chen J., Chen M., Li C.;
RT "Mutation of the rice Narrow leaf1 gene, which encodes a novel protein,
RT affects vein patterning and polar auxin transport.";
RL Plant Physiol. 147:1947-1959(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP FUNCTION.
RX PubMed=22179305; DOI=10.1007/s00299-011-1207-7;
RA Chen M., Luo J., Shao G., Wei X., Tang S., Sheng Z., Song J., Hu P.;
RT "Fine mapping of a major QTL for flag leaf width in rice, qFLW4, which
RT might be caused by alternative splicing of NAL1.";
RL Plant Cell Rep. 31:863-872(2012).
RN [8]
RP FUNCTION, BIOTECHNOLOGY, AND POLYMORPHISM.
RX PubMed=23985993; DOI=10.1038/srep02149;
RA Takai T., Adachi S., Taguchi-Shiobara F., Sanoh-Arai Y., Iwasawa N.,
RA Yoshinaga S., Hirose S., Taniguchi Y., Yamanouchi U., Wu J., Matsumoto T.,
RA Sugimoto K., Kondo K., Ikka T., Ando T., Kono I., Ito S., Shomura A.,
RA Ookawa T., Hirasawa T., Yano M., Kondo M., Yamamoto T.;
RT "A natural variant of NAL1, selected in high-yield rice breeding programs,
RT pleiotropically increases photosynthesis rate.";
RL Sci. Rep. 3:2149-2149(2013).
RN [9]
RP FUNCTION, BIOTECHNOLOGY, AND MUTAGENESIS OF HIS-233; VAL-475 AND ILE-484.
RX PubMed=24297875; DOI=10.1073/pnas.1310790110;
RA Fujita D., Trijatmiko K.R., Tagle A.G., Sapasap M.V., Koide Y., Sasaki K.,
RA Tsakirpaloglou N., Gannaban R.B., Nishimura T., Yanagihara S., Fukuta Y.,
RA Koshiba T., Slamet-Loedin I.H., Ishimaru T., Kobayashi N.;
RT "NAL1 allele from a rice landrace greatly increases yield in modern indica
RT cultivars.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:20431-20436(2013).
RN [10]
RP FUNCTION, BIOTECHNOLOGY, AND MUTAGENESIS OF HIS-233; VAL-475 AND ILE-484.
RX PubMed=24795339; DOI=10.1093/mp/ssu055;
RA Zhang G.H., Li S.Y., Wang L., Ye W.J., Zeng D.L., Rao Y.C., Peng Y.L.,
RA Hu J., Yang Y.L., Xu J., Ren D.Y., Gao Z.Y., Zhu L., Dong G.J., Hu X.M.,
RA Yan M.X., Guo L.B., Li C.Y., Qian Q.;
RT "LSCHL4 from japonica cultivar, which is allelic to NAL1, increases yield
RT of indica super rice 93-11.";
RL Mol. Plant 7:1350-1364(2014).
CC -!- FUNCTION: Involved in the regulation of lateral leaf growth
CC (PubMed:18562767, PubMed:22179305, PubMed:23985993). May be involved in
CC the regulation of basipetal polar auxin transport (PAT) and vascular
CC patterning in leaves (PubMed:18562767). Controls photosynthesis rate by
CC regulating carboxylation efficiency and consequently photosynthesis
CC rate (PubMed:23985993). Controls panicle and spikelet numbers, and
CC grain yield (PubMed:23985993, PubMed:24297875, PubMed:24795339).
CC {ECO:0000269|PubMed:18562767, ECO:0000269|PubMed:22179305,
CC ECO:0000269|PubMed:23985993, ECO:0000269|PubMed:24297875,
CC ECO:0000269|PubMed:24795339}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:18562767}. Cytoplasm {ECO:0000269|PubMed:18562767}.
CC Note=Expressed in discrete structures which appeared similar to nuclear
CC protein bodies. {ECO:0000269|PubMed:18562767}.
CC -!- TISSUE SPECIFICITY: Expressed in leaf sheaths, leaf blades, culms and
CC panicles (PubMed:18562767). Preferentially expressed in vascular
CC tissues in leaves and culms (PubMed:18562767).
CC {ECO:0000269|PubMed:18562767}.
CC -!- POLYMORPHISM: A partial loss-of-function allele of NAL1 is associated
CC with high photosynthesis rate, high number of spikelets and high grain
CC yield. {ECO:0000269|PubMed:23985993}.
CC -!- DISRUPTION PHENOTYPE: Narrow leaf phenotype (PubMed:18562767). Reduced
CC leaf blade width and plant height (PubMed:18562767). Altered internode
CC elongation pattern (PubMed:18562767). Altered vascular patterns in
CC leaves and culms (PubMed:18562767). {ECO:0000269|PubMed:18562767}.
CC -!- BIOTECHNOLOGY: Utilization of photosynthesis-related QTLs can enhance
CC high-yield breeding and provide a new strategy for achieving increased
CC rice productivity. {ECO:0000269|PubMed:23985993,
CC ECO:0000269|PubMed:24297875, ECO:0000269|PubMed:24795339}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF15780.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAS90996.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; EU093963; ABW89000.1; -; mRNA.
DR EMBL; AP008210; BAF15780.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014960; BAS90996.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM000141; EEE61678.1; -; Genomic_DNA.
DR RefSeq; XP_015635854.1; XM_015780368.1.
DR RefSeq; XP_015635855.1; XM_015780369.1.
DR RefSeq; XP_015635856.1; XM_015780370.1.
DR RefSeq; XP_015635857.1; XM_015780371.1.
DR RefSeq; XP_015635858.1; XM_015780372.1.
DR AlphaFoldDB; B4XT64; -.
DR STRING; 4530.OS04T0615000-01; -.
DR EnsemblPlants; Os04t0615000-01; Os04t0615000-01; Os04g0615000.
DR GeneID; 4336986; -.
DR Gramene; Os04t0615000-01; Os04t0615000-01; Os04g0615000.
DR KEGG; osa:4336986; -.
DR eggNOG; ENOG502QQZI; Eukaryota.
DR OrthoDB; 409922at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB.
DR GO; GO:0080006; P:internode patterning; IMP:UniProtKB.
DR GO; GO:0010305; P:leaf vascular tissue pattern formation; IMP:UniProtKB.
DR GO; GO:2000024; P:regulation of leaf development; IMP:UniProtKB.
DR GO; GO:0010222; P:stem vascular tissue pattern formation; IMP:UniProtKB.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR SUPFAM; SSF50494; SSF50494; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Nucleus; Reference proteome.
FT CHAIN 1..582
FT /note="Protein NARROW LEAF 1"
FT /id="PRO_0000445264"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 531..582
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 567..573
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 9..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 540..554
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..582
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 233
FT /note="H->R: Reduced grain yield; when associated with A-
FT 475 and V-484."
FT /evidence="ECO:0000269|PubMed:24297875,
FT ECO:0000269|PubMed:24795339"
FT MUTAGEN 475
FT /note="V->A: Reduced grain yield; when associated with R-
FT 233 and V-484."
FT /evidence="ECO:0000269|PubMed:24297875,
FT ECO:0000269|PubMed:24795339"
FT MUTAGEN 484
FT /note="I->V: Reduced grain yield; when associated with R-
FT 233 and A-475."
FT /evidence="ECO:0000269|PubMed:24297875,
FT ECO:0000269|PubMed:24795339"
SQ SEQUENCE 582 AA; 63252 MW; 25CF326B53D58185 CRC64;
MKPSDDKAQL SGLAQSEESS LDVDHQSFPC SPSIQPVASG CTHTENSAAY FLWPTSNLQH
CAAEGRANYF GNLQKGLLPR HPGRLPKGQQ ANSLLDLMTI RAFHSKILRR FSLGTAVGFR
IRKGDLTDIP AILVFVARKV HKKWLNPAQC LPAILEGPGG VWCDVDVVEF SYYGAPAQTP
KEQMFSELVD KLCGSDECIG SGSQVASHET FGTLGAIVKR RTGNKQVGFL TNHHVAVDLD
YPNQKMFHPL PPNLGPGVYL GAVERATSFI TDDVWYGIYA GTNPETFVRA DGAFIPFADD
FDISTVTTVV RGVGDIGDVK VIDLQCPLNS LIGRQVCKVG RSSGHTTGTV MAYALEYNDE
KGICFFTDIL VVGENRQTFD LEGDSGSLII LTSQDGEKPR PIGIIWGGTA NRGRLKLTSD
HGPENWTSGV DLGRLLDRLE LDIIITNESL QDAVQQQRFA LVAAVTSAVG ESSGVPVAIP
EEKIEEIFEP LGIQIQQLPR HDVAASGTEG EEASNTVVNV EEHQFISNFV GMSPVRDDQD
APRSITNLNN PSEEELAMSL HLGDREPKRL RSDSGSSLDL EK
