ID   NAMB2_TRITD             Reviewed;         396 AA.
AC   A0SPJ6;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=NAC transcription factor NAM-B2;
DE            Short=TtNAM-B2;
GN   Name=NAM-B2; Synonyms=GPC-B2;
OS   Triticum turgidum subsp. durum (Durum wheat) (Triticum durum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4567;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=17124321; DOI=10.1126/science.1133649;
RA   Uauy C., Distelfeld A., Fahima T., Blechl A., Dubcovsky J.;
RT   "A NAC Gene regulating senescence improves grain protein, zinc, and iron
RT   content in wheat.";
RL   Science 314:1298-1301(2006).
RN   [2]
RP   FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=22278768; DOI=10.1007/s11103-012-9881-6;
RA   Distelfeld A., Pearce S.P., Avni R., Scherer B., Uauy C., Piston F.,
RA   Slade A., Zhao R., Dubcovsky J.;
RT   "Divergent functions of orthologous NAC transcription factors in wheat and
RT   rice.";
RL   Plant Mol. Biol. 78:515-524(2012).
CC   -!- FUNCTION: Transcription factor of the NAC family associated with the
CC       grain protein content (GPC). Accelerates senescence and increases
CC       nutrient remobilization from leaves to developing grains. The
CC       tetraploid cultivated wheat (T.durum) contains one additional gene
CC       coding for a functional protein (NAM-A1) and one extra pseudogene (NAM-
CC       B1) (PubMed:17124321). Plays a weaker role in terminal senescence than
CC       NAM-A1. {ECO:0000269|PubMed:17124321, ECO:0000269|PubMed:22278768}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Highest expression in flag leaves. Expressed in
CC       green spikes, peduncles and stamens. {ECO:0000269|PubMed:17124321,
CC       ECO:0000269|PubMed:22278768}.
CC   -!- DEVELOPMENTAL STAGE: Increased expression during grain maturation and
CC       senescence. Not induced during flower development.
CC       {ECO:0000269|PubMed:17124321, ECO:0000269|PubMed:22278768}.
CC   -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC       domain.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC       {ECO:0000269|PubMed:22278768}.
CC   -!- MISCELLANEOUS: High grain protein content (GPC) is a desirable trait in
CC       breadmaking and pasta wheat varieties because of its positive effects
CC       on quality and nutritional value while Low GPC is a desirable trait for
CC       barley malt and beer production.
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DR   EMBL; DQ869676; ABI94355.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0SPJ6; -.
DR   SMR; A0SPJ6; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 2.170.150.80; -; 1.
DR   InterPro; IPR003441; NAC-dom.
DR   InterPro; IPR036093; NAC_dom_sf.
DR   Pfam; PF02365; NAM; 1.
DR   SUPFAM; SSF101941; SSF101941; 1.
DR   PROSITE; PS51005; NAC; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..396
FT                   /note="NAC transcription factor NAM-B2"
FT                   /id="PRO_0000420373"
FT   DOMAIN          28..193
FT                   /note="NAC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT   DNA_BIND        128..199
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT   REGION          1..33
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        12..26
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   396 AA;  42651 MW;  9759B8E05206A20D CRC64;
     MGSSDSSSGS APPRHQPPPP QQGSAPELPP GFRFHPTDEE LVVHYLKKKA AKVPLPVTII
     TEVDLYKFDP WELPEKATFG EQEWYFFSPR DRKYPNGARP NRAATSGYWK ATGTDKPILA
     SGCGREKVGV KKALVFYRGK PPKGLKTNWI MHEYRLTDAS SSATTSRPPP VTGGSRSASL
     RLDDWVLCRI YKKINKAAAG DQQRSMECED SVEDAVTAYP LYATAGMTGA GAHGSNYDSL
     LHHQDSHEDN FLDGLLTAED AGLSAGPTSL SHLAAAARAS PAPTKQFLAP SSSTPFNWLD
     ASTVGILPQA RNFPGFNRSR NVGNMSLSST ADMAVDNGGG NAINTMPPFM NHLPMQDGTY
     HQQHVILGAP LAPEATAAAT SAFQHPVQIS GVNWNP