位置:首页 > 蛋白库 > NAMPT_RAT
NAMPT_RAT
ID   NAMPT_RAT               Reviewed;         491 AA.
AC   Q80Z29; Q2VT47;
DT   05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=Nicotinamide phosphoribosyltransferase;
DE            Short=NAmPRTase;
DE            Short=Nampt;
DE            EC=2.4.2.12 {ECO:0000250|UniProtKB:Q99KQ4};
DE   AltName: Full=Pre-B-cell colony-enhancing factor 1 homolog;
DE            Short=PBEF;
DE   AltName: Full=Visfatin;
GN   Name=Nampt; Synonyms=Pbef1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=12782293; DOI=10.1016/s0014-5793(03)00476-9;
RA   Kitani T., Okuno S., Fujisawa H.;
RT   "Growth phase-dependent changes in the subcellular localization of pre-B-
RT   cell colony-enhancing factor.";
RL   FEBS Lett. 544:74-78(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Brain;
RA   Xing J., Gao Y., Cao G., Chen J.;
RT   "NAD biosynthesis pathway mediated by nicotinamide
RT   phosphoribosyltransferase attenuates ischemic neuronal cell death.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEXES WITH NICOTINAMIDE
RP   MONONUCLEOTIDE AND FK866, AND SUBUNIT.
RX   PubMed=16901503; DOI=10.1016/j.jmb.2006.06.082;
RA   Kim M.-K., Lee J.H., Kim H., Park S.J., Kim S.H., Kang G.B., Lee Y.S.,
RA   Kim J.B., Kim K.K., Suh S.W., Eom S.H.;
RT   "Crystal structure of visfatin/pre-B cell colony-enhancing factor
RT   1/nicotinamide phosphoribosyltransferase, free and in complex with the
RT   anti-cancer agent FK-866.";
RL   J. Mol. Biol. 362:66-77(2006).
CC   -!- FUNCTION: Catalyzes the condensation of nicotinamide with 5-
CC       phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an
CC       intermediate in the biosynthesis of NAD. It is the rate limiting
CC       component in the mammalian NAD biosynthesis pathway. The secreted form
CC       behaves both as a cytokine with immunomodulating properties and an
CC       adipokine with anti-diabetic properties, it has no enzymatic activity,
CC       partly because of lack of activation by ATP, which has a low level in
CC       extracellular space and plasma. Plays a role in the modulation of
CC       circadian clock function. NAMPT-dependent oscillatory production of NAD
CC       regulates oscillation of clock target gene expression by releasing the
CC       core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent
CC       SIRT1-mediated suppression. {ECO:0000250|UniProtKB:P43490,
CC       ECO:0000250|UniProtKB:Q99KQ4}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-nicotinamide D-ribonucleotide + diphosphate = 5-phospho-
CC         alpha-D-ribose 1-diphosphate + H(+) + nicotinamide;
CC         Xref=Rhea:RHEA:16149, ChEBI:CHEBI:14649, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17154, ChEBI:CHEBI:33019, ChEBI:CHEBI:58017; EC=2.4.2.12;
CC         Evidence={ECO:0000250|UniProtKB:Q99KQ4};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:16151;
CC         Evidence={ECO:0000250|UniProtKB:Q99KQ4};
CC   -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-
CC       ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and
CC       nicotinamide: step 1/1.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:16901503}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12782293}. Cytoplasm
CC       {ECO:0000269|PubMed:12782293}. Secreted {ECO:0000250|UniProtKB:P43490}.
CC       Note=Under non-inflammatory conditions, visfatin predominantly exhibits
CC       a granular pattern within the nucleus. Secreted by endothelial cells
CC       upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-
CC       fold higher concentrations compared to maternal serum.
CC       {ECO:0000250|UniProtKB:P43490}.
CC   -!- TISSUE SPECIFICITY: Expressed in various tissues. At the highest level
CC       in liver and at the second highest in heart. The amount is higher in
CC       heart than in lung. {ECO:0000269|PubMed:12782293}.
CC   -!- SIMILARITY: Belongs to the NAPRTase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB081730; BAC66022.1; -; mRNA.
DR   EMBL; AY831728; AAX49410.1; -; mRNA.
DR   EMBL; BC085681; AAH85681.1; -; mRNA.
DR   RefSeq; NP_808789.1; NM_177928.3.
DR   PDB; 2G95; X-ray; 1.90 A; A/B=1-491.
DR   PDB; 2G96; X-ray; 2.90 A; A/B=1-491.
DR   PDB; 2G97; X-ray; 2.90 A; A/B=1-491.
DR   PDB; 3G8E; X-ray; 3.00 A; A/B=1-491.
DR   PDBsum; 2G95; -.
DR   PDBsum; 2G96; -.
DR   PDBsum; 2G97; -.
DR   PDBsum; 3G8E; -.
DR   AlphaFoldDB; Q80Z29; -.
DR   SMR; Q80Z29; -.
DR   STRING; 10116.ENSRNOP00000013043; -.
DR   BindingDB; Q80Z29; -.
DR   ChEMBL; CHEMBL3259475; -.
DR   iPTMnet; Q80Z29; -.
DR   PhosphoSitePlus; Q80Z29; -.
DR   jPOST; Q80Z29; -.
DR   PaxDb; Q80Z29; -.
DR   PRIDE; Q80Z29; -.
DR   Ensembl; ENSRNOT00000013043; ENSRNOP00000013043; ENSRNOG00000009754.
DR   GeneID; 297508; -.
DR   KEGG; rno:297508; -.
DR   UCSC; RGD:631365; rat.
DR   CTD; 10135; -.
DR   RGD; 631365; Nampt.
DR   eggNOG; ENOG502QSGN; Eukaryota.
DR   GeneTree; ENSGT00940000153456; -.
DR   HOGENOM; CLU_012550_2_0_1; -.
DR   InParanoid; Q80Z29; -.
DR   OMA; TFGFAMK; -.
DR   OrthoDB; 446086at2759; -.
DR   BRENDA; 2.4.2.12; 5301.
DR   Reactome; R-RNO-197264; Nicotinamide salvaging.
DR   SABIO-RK; Q80Z29; -.
DR   UniPathway; UPA00253; UER00890.
DR   EvolutionaryTrace; Q80Z29; -.
DR   PRO; PR:Q80Z29; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Bgee; ENSRNOG00000009754; Expressed in skeletal muscle tissue and 19 other tissues.
DR   ExpressionAtlas; Q80Z29; baseline and differential.
DR   Genevisible; Q80Z29; RN.
DR   GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IDA:RGD.
DR   GO; GO:0005886; C:plasma membrane; IDA:RGD.
DR   GO; GO:0005125; F:cytokine activity; IDA:RGD.
DR   GO; GO:1901363; F:heterocyclic compound binding; IDA:RGD.
DR   GO; GO:0042802; F:identical protein binding; IDA:RGD.
DR   GO; GO:0047280; F:nicotinamide phosphoribosyltransferase activity; IDA:RGD.
DR   GO; GO:0004514; F:nicotinate-nucleotide diphosphorylase (carboxylating) activity; IEA:InterPro.
DR   GO; GO:0000166; F:nucleotide binding; IDA:RGD.
DR   GO; GO:0007568; P:aging; IEP:RGD.
DR   GO; GO:1904646; P:cellular response to amyloid-beta; IEP:RGD.
DR   GO; GO:0071479; P:cellular response to ionizing radiation; IEP:RGD.
DR   GO; GO:0090650; P:cellular response to oxygen-glucose deprivation; IEP:RGD.
DR   GO; GO:0032922; P:circadian regulation of gene expression; ISS:UniProtKB.
DR   GO; GO:0007623; P:circadian rhythm; IEP:RGD.
DR   GO; GO:0007565; P:female pregnancy; IEP:RGD.
DR   GO; GO:0001774; P:microglial cell activation; IMP:RGD.
DR   GO; GO:0009435; P:NAD biosynthetic process; ISO:RGD.
DR   GO; GO:0010507; P:negative regulation of autophagy; IMP:RGD.
DR   GO; GO:2000773; P:negative regulation of cellular senescence; IDA:RGD.
DR   GO; GO:0070997; P:neuron death; IEP:RGD.
DR   GO; GO:0051770; P:positive regulation of nitric-oxide synthase biosynthetic process; ISO:RGD.
DR   GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; IMP:RGD.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR   GO; GO:0014916; P:regulation of lung blood pressure; IMP:RGD.
DR   GO; GO:1905377; P:response to D-galactose; IEP:RGD.
DR   GO; GO:0014070; P:response to organic cyclic compound; IEP:RGD.
DR   CDD; cd01569; PBEF_like; 1.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR041529; DUF5598.
DR   InterPro; IPR041525; N/Namide_PRibTrfase.
DR   InterPro; IPR016471; Nicotinamide_PRibTrfase.
DR   InterPro; IPR036068; Nicotinate_pribotase-like_C.
DR   PANTHER; PTHR43816; PTHR43816; 1.
DR   Pfam; PF18127; NAMPT_N; 1.
DR   Pfam; PF04095; NAPRTase; 1.
DR   PIRSF; PIRSF005943; NMPRT; 1.
DR   SUPFAM; SSF51690; SSF51690; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Biological rhythms; Cytokine; Cytoplasm;
KW   Glycosyltransferase; Nucleus; Phosphoprotein;
KW   Pyridine nucleotide biosynthesis; Reference proteome; Secreted;
KW   Transferase.
FT   CHAIN           1..491
FT                   /note="Nicotinamide phosphoribosyltransferase"
FT                   /id="PRO_0000205866"
FT   BINDING         196
FT                   /ligand="diphosphate"
FT                   /ligand_id="ChEBI:CHEBI:33019"
FT                   /evidence="ECO:0000250"
FT   BINDING         219
FT                   /ligand="beta-nicotinamide D-ribonucleotide"
FT                   /ligand_id="ChEBI:CHEBI:14649"
FT                   /evidence="ECO:0000250"
FT   BINDING         247
FT                   /ligand="diphosphate"
FT                   /ligand_id="ChEBI:CHEBI:33019"
FT                   /evidence="ECO:0000250"
FT   BINDING         311..313
FT                   /ligand="beta-nicotinamide D-ribonucleotide"
FT                   /ligand_id="ChEBI:CHEBI:14649"
FT   BINDING         311
FT                   /ligand="diphosphate"
FT                   /ligand_id="ChEBI:CHEBI:33019"
FT                   /evidence="ECO:0000250"
FT   BINDING         353..354
FT                   /ligand="beta-nicotinamide D-ribonucleotide"
FT                   /ligand_id="ChEBI:CHEBI:14649"
FT   BINDING         384
FT                   /ligand="beta-nicotinamide D-ribonucleotide"
FT                   /ligand_id="ChEBI:CHEBI:14649"
FT   BINDING         392
FT                   /ligand="beta-nicotinamide D-ribonucleotide"
FT                   /ligand_id="ChEBI:CHEBI:14649"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:P43490"
FT   MOD_RES         188
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P43490"
FT   MOD_RES         472
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P43490"
FT   HELIX           11..13
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          14..16
FT                   /evidence="ECO:0007829|PDB:2G96"
FT   HELIX           17..24
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          30..39
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          56..58
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           62..69
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          71..73
FT                   /evidence="ECO:0007829|PDB:2G97"
FT   HELIX           77..91
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           98..108
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          114..118
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          124..138
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           139..143
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           144..147
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           149..153
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           156..180
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           186..188
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          189..192
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           195..197
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          198..200
FT                   /evidence="ECO:0007829|PDB:2G96"
FT   HELIX           201..211
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   TURN            212..214
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          217..219
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           222..230
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          234..236
FT                   /evidence="ECO:0007829|PDB:2G97"
FT   STRAND          238..240
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           247..250
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           251..253
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           255..257
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           258..268
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          270..272
FT                   /evidence="ECO:0007829|PDB:2G96"
FT   STRAND          274..278
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           283..288
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           289..294
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           296..299
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          308..311
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           317..331
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          348..352
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           358..370
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           375..377
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          378..383
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           384..387
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           392..394
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          397..406
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          409..411
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           421..423
FT                   /evidence="ECO:0007829|PDB:2G96"
FT   STRAND          431..434
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          440..443
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           447..450
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   STRAND          453..455
FT                   /evidence="ECO:0007829|PDB:2G97"
FT   STRAND          459..463
FT                   /evidence="ECO:0007829|PDB:2G95"
FT   HELIX           473..479
FT                   /evidence="ECO:0007829|PDB:2G95"
SQ   SEQUENCE   491 AA;  55438 MW;  78F526F0D3F579FC CRC64;
     MNAAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKVR KVKYEETVFY
     GLQYILNKYL KGKVVTKEKI QEAKEVYREH FQDDVFNERG WNYILEKYDG HLPIEVKAVP
     EGSVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS
     GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGIALIKKY YGTKDPVPGY
     SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV
     SRSTEAPLII RPDSGNPLDT VLKVLDILGK KFPVSENSKG YKLLPPYLRV IQGDGVDINT
     LQEIVEGMKQ KKWSIENVSF GSGGALLQKL TRDLLNCSFK CSYVVTNGLG VNVFKDPVAD
     PNKRSKKGRL SLHRTPAGTF VTLEEGKGDL EEYGHDLLHT VFKNGKVTKS YSFDEVRKNA
     QLNMEQDVAP H
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024