NAMT1_ORYSJ
ID NAMT1_ORYSJ Reviewed; 365 AA.
AC Q6K9X3; A0A0P0VRE1;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Nicotinate N-methyltransferase 1 {ECO:0000305};
DE EC=2.1.1.7 {ECO:0000269|PubMed:28533213};
GN Name=NANMT1 {ECO:0000305};
GN OrderedLocusNames=Os02g0823400 {ECO:0000312|EMBL:BAS81660.1},
GN LOC_Os02g57760 {ECO:0000305};
GN ORFNames=OJ1063_D06.1 {ECO:0000312|EMBL:BAD22855.1},
GN OJ1136_C04.9 {ECO:0000312|EMBL:BAD22923.1},
GN OsJ_08933 {ECO:0000312|EMBL:EEE58073.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=28533213; DOI=10.1104/pp.17.00259;
RA Li W., Zhang F., Wu R., Jia L., Li G., Guo Y., Liu C., Wang G.;
RT "A novel N-methyltransferase in Arabidopsis appears to feed a conserved
RT pathway for nicotinate detoxification among land plants and is associated
RT with lignin biosynthesis.";
RL Plant Physiol. 174:1492-1504(2017).
CC -!- FUNCTION: Involved in nicotinate detoxification in planta
CC (PubMed:28533213). Catalyzes the conversion of nicotinate to N-
CC methylnicotinate, which is a detoxified form of endogenous nicotinate
CC in planta (PubMed:28533213). {ECO:0000269|PubMed:28533213}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=nicotinate + S-adenosyl-L-methionine = N-methylnicotinate + S-
CC adenosyl-L-homocysteine; Xref=Rhea:RHEA:20241, ChEBI:CHEBI:18123,
CC ChEBI:CHEBI:32544, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.7;
CC Evidence={ECO:0000269|PubMed:28533213};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20242;
CC Evidence={ECO:0000269|PubMed:28533213};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=43.62 uM for nicotinate {ECO:0000269|PubMed:28533213};
CC KM=67.26 uM for S-adenosyl-L-methionine
CC {ECO:0000269|PubMed:28533213};
CC Note=kcat is 2.25 sec(-1) with nicotinate as substrate
CC (PubMed:28533213). kcat is 2.07 sec(-1) with S-adenosyl-L-methionine
CC as substrate (PubMed:28533213). {ECO:0000269|PubMed:28533213};
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Cation-independent O-methyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AP003989; BAD22855.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF10483.1; -; Genomic_DNA.
DR EMBL; AP004026; BAD22923.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS81660.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE58073.1; -; Genomic_DNA.
DR EMBL; AK105029; BAG97074.1; -; mRNA.
DR RefSeq; XP_015627175.1; XM_015771689.1.
DR AlphaFoldDB; Q6K9X3; -.
DR SMR; Q6K9X3; -.
DR STRING; 4530.OS02T0823400-01; -.
DR PaxDb; Q6K9X3; -.
DR PRIDE; Q6K9X3; -.
DR EnsemblPlants; Os02t0823400-01; Os02t0823400-01; Os02g0823400.
DR GeneID; 4331197; -.
DR Gramene; Os02t0823400-01; Os02t0823400-01; Os02g0823400.
DR KEGG; osa:4331197; -.
DR eggNOG; KOG3178; Eukaryota.
DR HOGENOM; CLU_005533_12_1_1; -.
DR InParanoid; Q6K9X3; -.
DR OMA; IYIDYFY; -.
DR OrthoDB; 817726at2759; -.
DR PlantReactome; R-OSA-1119316; Phenylpropanoid biosynthesis.
DR PlantReactome; R-OSA-1119528; Beta-alanine betaine biosynthesis.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0008938; F:nicotinate N-methyltransferase activity; IDA:UniProtKB.
DR GO; GO:0008171; F:O-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IBA:GO_Central.
DR GO; GO:0019438; P:aromatic compound biosynthetic process; IBA:GO_Central.
DR GO; GO:0032259; P:methylation; IBA:GO_Central.
DR GO; GO:1901847; P:nicotinate metabolic process; IDA:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR016461; COMT-like.
DR InterPro; IPR001077; O_MeTrfase_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11746; PTHR11746; 1.
DR Pfam; PF00891; Methyltransf_2; 1.
DR PIRSF; PIRSF005739; O-mtase; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51683; SAM_OMT_II; 1.
PE 1: Evidence at protein level;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..365
FT /note="Nicotinate N-methyltransferase 1"
FT /id="PRO_0000451461"
FT BINDING 232
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
SQ SEQUENCE 365 AA; 38647 MW; AFDE2F63F01626A8 CRC64;
MGGGGDGELS PAEARLAMME LANMISVPMA LTAVIRLGVP AKLWAGGANA PLAAADLLPA
GHPDPSVLER LLRLLASRGV FSEHTGSSSP SPRRFSLTAV GRTLVPGGGG SPSGSGASYA
DYVLQHHQDA LVRAWPLLHE AVLDPSGPEP FARANAGVPA YAYYGKDREA NEVMLRAMTG
VSEPFMEALL EGYGDGGFEG VSTLVDVGGS SGACLEMIMR RVRTIRDGVN FDLPDVVAAA
PPIPGVRHVG GDMFKSIPSG DAIFMKWVLT TWTNEECTAI LSNCHKALPG GGKVIACEPV
VPDTTDGSTR TRALLENDIF VMATYRTQGR ERSEEEFRHL GLAAGFASFR AIYLDPFYAV
LEYTK
