NAMT1_SOYBN
ID NAMT1_SOYBN Reviewed; 355 AA.
AC I1M2U5;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Nicotinate N-methyltransferase 1 {ECO:0000305};
DE EC=2.1.1.7 {ECO:0000269|PubMed:28533213};
GN Name=NANMT1 {ECO:0000305};
GN ORFNames=GLYMA_13G263200 {ECO:0000312|EMBL:KRH21851.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82;
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=28533213; DOI=10.1104/pp.17.00259;
RA Li W., Zhang F., Wu R., Jia L., Li G., Guo Y., Liu C., Wang G.;
RT "A novel N-methyltransferase in Arabidopsis appears to feed a conserved
RT pathway for nicotinate detoxification among land plants and is associated
RT with lignin biosynthesis.";
RL Plant Physiol. 174:1492-1504(2017).
CC -!- FUNCTION: Involved in nicotinate detoxification in planta
CC (PubMed:28533213). Catalyzes the conversion of nicotinate to N-
CC methylnicotinate, which is a detoxified form of endogenous nicotinate
CC in planta (PubMed:28533213). {ECO:0000269|PubMed:28533213}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=nicotinate + S-adenosyl-L-methionine = N-methylnicotinate + S-
CC adenosyl-L-homocysteine; Xref=Rhea:RHEA:20241, ChEBI:CHEBI:18123,
CC ChEBI:CHEBI:32544, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.7;
CC Evidence={ECO:0000269|PubMed:28533213};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:20242;
CC Evidence={ECO:0000269|PubMed:28533213};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=55.86 uM for nicotinate {ECO:0000269|PubMed:28533213};
CC KM=69.2 uM for S-adenosyl-L-methionine {ECO:0000269|PubMed:28533213};
CC Note=kcat is 4.81 sec(-1) with nicotinate as substrate
CC (PubMed:28533213). kcat is 4.14 sec(-1) with S-adenosyl-L-methionine
CC as substrate (PubMed:28533213). {ECO:0000269|PubMed:28533213};
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. Cation-independent O-methyltransferase family.
CC {ECO:0000305}.
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DR EMBL; CM000846; KRH21851.1; -; Genomic_DNA.
DR EMBL; CM000846; KRH21852.1; -; Genomic_DNA.
DR RefSeq; XP_006594704.1; XM_006594641.2.
DR AlphaFoldDB; I1M2U5; -.
DR SMR; I1M2U5; -.
DR STRING; 3847.GLYMA13G33830.1; -.
DR PRIDE; I1M2U5; -.
DR EnsemblPlants; KRH21851; KRH21851; GLYMA_13G263200.
DR EnsemblPlants; KRH21852; KRH21852; GLYMA_13G263200.
DR Gramene; KRH21851; KRH21851; GLYMA_13G263200.
DR Gramene; KRH21852; KRH21852; GLYMA_13G263200.
DR eggNOG; KOG3178; Eukaryota.
DR HOGENOM; CLU_005533_12_1_1; -.
DR InParanoid; I1M2U5; -.
DR OMA; IYIDYFY; -.
DR OrthoDB; 817726at2759; -.
DR Proteomes; UP000008827; Chromosome 13.
DR GO; GO:0008938; F:nicotinate N-methyltransferase activity; IDA:UniProtKB.
DR GO; GO:0008171; F:O-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IBA:GO_Central.
DR GO; GO:0019438; P:aromatic compound biosynthetic process; IBA:GO_Central.
DR GO; GO:0032259; P:methylation; IBA:GO_Central.
DR GO; GO:1901847; P:nicotinate metabolic process; IDA:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR016461; COMT-like.
DR InterPro; IPR001077; O_MeTrfase_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11746; PTHR11746; 1.
DR Pfam; PF00891; Methyltransf_2; 1.
DR PIRSF; PIRSF005739; O-mtase; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51683; SAM_OMT_II; 1.
PE 1: Evidence at protein level;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..355
FT /note="Nicotinate N-methyltransferase 1"
FT /id="PRO_0000451462"
FT BINDING 222
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01020"
SQ SEQUENCE 355 AA; 39914 MW; BD29CC951B1A13BE CRC64;
MEKEESTEQR KQARLAIMEL ANMISVPMAL NAVVRLNVAD AIWQGGANNP LSAAEILPRL
LPAGGGDAEN LQRLLRMLAS YGVFYEHLSA GERKYSLTDV GKTLVTDEQG LSYAHYVLQH
HQDALMRAWP MVHEAVVDPT KEPFERANGE PAYGYYLKHP EMNDLMVRAM SGVSVPFIRA
MLEGYDGFQG VEKLVDVGGS GGDCLRMILE KHPTIKEGIN FDLPEVVAKA PQIPFVTHVG
GDMFKFIPQG DAIFMKWVLT TWTDEECKHI MQNCHKALPE GGKLIACEPV LPEDSDESHR
TRALLEGDIF VMTIYRAKGK HRTEEQFRQL AIDAGFPRFR AFHVDHFYTV LEFQK
