NANB_STRPN
ID NANB_STRPN Reviewed; 697 AA.
AC Q54727;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Sialidase B;
DE EC=3.2.1.18;
DE AltName: Full=Neuraminidase B;
DE Flags: Precursor;
GN Name=nanB; OrderedLocusNames=SP_1687;
OS Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=170187;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Serotype 6;
RX PubMed=8759848; DOI=10.1128/jb.178.16.4854-4860.1996;
RA Berry A.M., Lock R.A., Paton J.C.;
RT "Cloning and characterization of nanB, a second Streptococcus pneumoniae
RT neuraminidase gene, and purification of the NanB enzyme from recombinant
RT Escherichia coli.";
RL J. Bacteriol. 178:4854-4860(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-334 / TIGR4;
RX PubMed=11463916; DOI=10.1126/science.1061217;
RA Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT "Complete genome sequence of a virulent isolate of Streptococcus
RT pneumoniae.";
RL Science 293:498-506(2001).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-
CC (2->8)- glycosidic linkages of terminal sialic acid residues in
CC oligosaccharides, glycoproteins, glycolipids, colominic acid and
CC synthetic substrates.; EC=3.2.1.18;
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 33 family. {ECO:0000305}.
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DR EMBL; U43526; AAC44396.1; -; Genomic_DNA.
DR EMBL; AE005672; AAK75766.1; -; Genomic_DNA.
DR PIR; E95196; E95196.
DR RefSeq; WP_001042179.1; NZ_AKVY01000001.1.
DR PDB; 2JKB; X-ray; 1.54 A; A=30-697.
DR PDB; 2VW0; X-ray; 2.30 A; A=1-697.
DR PDB; 2VW1; X-ray; 2.39 A; A=1-697.
DR PDB; 2VW2; X-ray; 1.70 A; A=1-697.
DR PDB; 4FOQ; X-ray; 1.99 A; A=1-697.
DR PDB; 4FOV; X-ray; 2.29 A; A=1-697.
DR PDB; 4FOW; X-ray; 2.10 A; A=1-697.
DR PDB; 4FOY; X-ray; 1.84 A; A=1-697.
DR PDB; 4FP2; X-ray; 2.05 A; A=1-697.
DR PDB; 4FP3; X-ray; 2.74 A; A=1-697.
DR PDB; 4FPC; X-ray; 2.32 A; A=1-697.
DR PDB; 4FPE; X-ray; 2.18 A; A=1-697.
DR PDB; 4FPF; X-ray; 2.23 A; A=1-697.
DR PDB; 4FPG; X-ray; 2.58 A; A=1-697.
DR PDB; 4FPH; X-ray; 1.94 A; A=1-697.
DR PDB; 4FPJ; X-ray; 1.98 A; A=1-697.
DR PDB; 4FPK; X-ray; 2.40 A; A=1-697.
DR PDB; 4FPL; X-ray; 2.10 A; A=1-697.
DR PDB; 4FPO; X-ray; 2.59 A; B=1-697.
DR PDB; 4FPY; X-ray; 2.18 A; A=1-697.
DR PDB; 4FQ4; X-ray; 1.84 A; A=1-697.
DR PDB; 4XE9; X-ray; 1.84 A; A=39-696.
DR PDB; 4XHB; X-ray; 1.88 A; A=39-696.
DR PDB; 4XHX; X-ray; 2.10 A; A=39-696.
DR PDB; 4XIK; X-ray; 1.91 A; A=39-696.
DR PDB; 4XIL; X-ray; 1.90 A; A=39-696.
DR PDB; 4XIO; X-ray; 1.80 A; A=39-696.
DR PDB; 4XJ8; X-ray; 2.75 A; A=39-696.
DR PDB; 4XJ9; X-ray; 1.98 A; A=39-696.
DR PDB; 4XJA; X-ray; 1.98 A; A=39-696.
DR PDB; 4XJU; X-ray; 1.94 A; A=39-696.
DR PDB; 4XJW; X-ray; 1.53 A; A=39-696.
DR PDB; 4XJZ; X-ray; 1.56 A; A=39-696.
DR PDB; 4XMA; X-ray; 2.09 A; A=39-696.
DR PDB; 4XMI; X-ray; 1.97 A; A=39-696.
DR PDB; 4XOG; X-ray; 2.09 A; A=37-694.
DR PDB; 4XYX; X-ray; 2.10 A; A=40-697.
DR PDBsum; 2JKB; -.
DR PDBsum; 2VW0; -.
DR PDBsum; 2VW1; -.
DR PDBsum; 2VW2; -.
DR PDBsum; 4FOQ; -.
DR PDBsum; 4FOV; -.
DR PDBsum; 4FOW; -.
DR PDBsum; 4FOY; -.
DR PDBsum; 4FP2; -.
DR PDBsum; 4FP3; -.
DR PDBsum; 4FPC; -.
DR PDBsum; 4FPE; -.
DR PDBsum; 4FPF; -.
DR PDBsum; 4FPG; -.
DR PDBsum; 4FPH; -.
DR PDBsum; 4FPJ; -.
DR PDBsum; 4FPK; -.
DR PDBsum; 4FPL; -.
DR PDBsum; 4FPO; -.
DR PDBsum; 4FPY; -.
DR PDBsum; 4FQ4; -.
DR PDBsum; 4XE9; -.
DR PDBsum; 4XHB; -.
DR PDBsum; 4XHX; -.
DR PDBsum; 4XIK; -.
DR PDBsum; 4XIL; -.
DR PDBsum; 4XIO; -.
DR PDBsum; 4XJ8; -.
DR PDBsum; 4XJ9; -.
DR PDBsum; 4XJA; -.
DR PDBsum; 4XJU; -.
DR PDBsum; 4XJW; -.
DR PDBsum; 4XJZ; -.
DR PDBsum; 4XMA; -.
DR PDBsum; 4XMI; -.
DR PDBsum; 4XOG; -.
DR PDBsum; 4XYX; -.
DR AlphaFoldDB; Q54727; -.
DR SMR; Q54727; -.
DR STRING; 170187.SP_1687; -.
DR ChEMBL; CHEMBL4295604; -.
DR CAZy; CBM40; Carbohydrate-Binding Module Family 40.
DR CAZy; GH33; Glycoside Hydrolase Family 33.
DR EnsemblBacteria; AAK75766; AAK75766; SP_1687.
DR KEGG; spn:SP_1687; -.
DR eggNOG; COG4409; Bacteria.
DR OMA; IFLMADM; -.
DR BioCyc; SPNE170187:G1FZB-1708-MON; -.
DR BRENDA; 3.2.1.18; 1960.
DR SABIO-RK; Q54727; -.
DR EvolutionaryTrace; Q54727; -.
DR PRO; PR:Q54727; -.
DR Proteomes; UP000000585; Chromosome.
DR GO; GO:0052794; F:exo-alpha-(2->3)-sialidase activity; IEA:UniProtKB-EC.
DR GO; GO:0052795; F:exo-alpha-(2->6)-sialidase activity; IEA:UniProtKB-EC.
DR GO; GO:0052796; F:exo-alpha-(2->8)-sialidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.40.220.10; -; 1.
DR InterPro; IPR002860; BNR_rpt.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR004124; Glyco_hydro_33_N.
DR InterPro; IPR011040; Sialidase.
DR InterPro; IPR026856; Sialidase_fam.
DR InterPro; IPR036278; Sialidase_sf.
DR InterPro; IPR023364; Trans_sialidase_dom3.
DR PANTHER; PTHR10628; PTHR10628; 1.
DR Pfam; PF02012; BNR; 1.
DR Pfam; PF13088; BNR_2; 1.
DR Pfam; PF02973; Sialidase; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF50939; SSF50939; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Glycosidase; Hydrolase; Repeat; Signal.
FT SIGNAL 1..29
FT CHAIN 30..697
FT /note="Sialidase B"
FT /id="PRO_0000012038"
FT REPEAT 280..291
FT /note="BNR 1"
FT REPEAT 462..473
FT /note="BNR 2"
FT REPEAT 517..528
FT /note="BNR 3"
FT REPEAT 566..577
FT /note="BNR 4"
FT ACT_SITE 270
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 541
FT /evidence="ECO:0000255"
FT ACT_SITE 653
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 245
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 557
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 619
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 121
FT /note="K -> E (in Ref. 1; AAC44396)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="D -> G (in Ref. 1; AAC44396)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="T -> N (in Ref. 1; AAC44396)"
FT /evidence="ECO:0000305"
FT CONFLICT 393
FT /note="N -> D (in Ref. 1; AAC44396)"
FT /evidence="ECO:0000305"
FT CONFLICT 639
FT /note="K -> R (in Ref. 1; AAC44396)"
FT /evidence="ECO:0000305"
FT STRAND 42..53
FT /evidence="ECO:0007829|PDB:4XJW"
FT HELIX 58..61
FT /evidence="ECO:0007829|PDB:4XJW"
FT TURN 62..64
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 70..77
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 83..91
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 101..107
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 112..118
FT /evidence="ECO:0007829|PDB:4XJW"
FT TURN 119..122
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 123..131
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 136..141
FT /evidence="ECO:0007829|PDB:2VW1"
FT STRAND 144..151
FT /evidence="ECO:0007829|PDB:4XJW"
FT TURN 152..155
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 156..161
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 164..170
FT /evidence="ECO:0007829|PDB:4XJW"
FT HELIX 177..179
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 185..193
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 196..199
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 202..213
FT /evidence="ECO:0007829|PDB:4XJW"
FT HELIX 217..220
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 242..245
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 257..266
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 271..273
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 275..284
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 293..296
FT /evidence="ECO:0007829|PDB:4XJW"
FT TURN 312..316
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 324..332
FT /evidence="ECO:0007829|PDB:4XJW"
FT TURN 334..336
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 339..346
FT /evidence="ECO:0007829|PDB:4XJW"
FT TURN 352..354
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 361..365
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 368..375
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 383..385
FT /evidence="ECO:0007829|PDB:4XJW"
FT HELIX 387..389
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 390..393
FT /evidence="ECO:0007829|PDB:4XJW"
FT TURN 394..397
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 398..404
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 408..412
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 418..425
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 432..441
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 446..448
FT /evidence="ECO:0007829|PDB:4FPG"
FT STRAND 450..452
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 458..466
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 467..469
FT /evidence="ECO:0007829|PDB:4FP3"
FT STRAND 483..485
FT /evidence="ECO:0007829|PDB:4FPO"
FT STRAND 492..494
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 499..502
FT /evidence="ECO:0007829|PDB:4XIL"
FT STRAND 504..510
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 513..521
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 527..532
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 542..548
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 551..556
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 559..562
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 564..570
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 573..575
FT /evidence="ECO:0007829|PDB:4FP3"
FT STRAND 594..597
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 607..613
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 616..620
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 622..629
FT /evidence="ECO:0007829|PDB:4XJW"
FT TURN 631..633
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 636..645
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 653..658
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 664..669
FT /evidence="ECO:0007829|PDB:4XJW"
FT STRAND 676..678
FT /evidence="ECO:0007829|PDB:2VW1"
FT STRAND 685..690
FT /evidence="ECO:0007829|PDB:4XJW"
FT HELIX 692..695
FT /evidence="ECO:0007829|PDB:4XJW"
SQ SEQUENCE 697 AA; 77695 MW; A0D9F9AFDE46AFD7 CRC64;
MNKRGLYSKL GISVVGISLL MGVPTLIHAN ELNYGQLSIS PIFQGGSYQL NNKSIDISSL
LLDKLSGESQ TVVMKFKADK PNSLQALFGL SNSKAGFKNN YFSIFMRDSG EIGVEIRDAQ
KGINYLFSRP ASLWGKHKGQ AVENTLVFVS DSKDKTYTMY VNGIEVFSET VDTFLPISNI
NGIDKATLGA VNREGKEHYL AKGSIDEISL FNKAISDQEV STIPLSNPFQ LIFQSGDSTQ
ANYFRIPTLY TLSSGRVLSS IDARYGGTHD SKSKINIATS YSDDNGKTWS EPIFAMKFND
YEEQLVYWPR DNKLKNSQIS GSASFIDSSI VEDKKSGKTI LLADVMPAGI GNNNANKADS
GFKEINGHYY LKLKKNGDND FRYTVRENGV VYNETTNKPT NYTINDKYEV LEGGKSLTVE
QYSVDFDSGS LRERHNGKQV PMNVFYKDSL FKVTPTNYIA MTTSQNRGES WEQFKLLPPF
LGEKHNGTYL CPGQGLALKS SNRLIFATYT SGELTYLISD DSGQTWKKSS ASIPFKNATA
EAQMVELRDG VIRTFFRTTT GKIAYMTSRD SGETWSKVSY IDGIQQTSYG TQVSAIKYSQ
LIDGKEAVIL STPNSRSGRK GGQLVVGLVN KEDDSIDWKY HYDIDLPSYG YAYSAITELP
NHHIGVLFEK YDSWSRNELH LSNVVQYIDL EINDLTK
