NANE_STAAN
ID NANE_STAAN Reviewed; 222 AA.
AC P65517; Q99WQ8;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 96.
DE RecName: Full=Putative N-acetylmannosamine-6-phosphate 2-epimerase {ECO:0000255|HAMAP-Rule:MF_01235};
DE EC=5.1.3.9 {ECO:0000255|HAMAP-Rule:MF_01235};
DE AltName: Full=ManNAc-6-P epimerase {ECO:0000255|HAMAP-Rule:MF_01235};
GN Name=nanE {ECO:0000255|HAMAP-Rule:MF_01235}; OrderedLocusNames=SA0307;
OS Staphylococcus aureus (strain N315).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=158879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N315;
RX PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA Hiramatsu K.;
RT "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL Lancet 357:1225-1240(2001).
CC -!- FUNCTION: Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-
CC acetylglucosamine-6-phosphate (GlcNAc-6-P). {ECO:0000255|HAMAP-
CC Rule:MF_01235}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acyl-D-glucosamine 6-phosphate = an N-acyl-D-mannosamine
CC 6-phosphate; Xref=Rhea:RHEA:23932, ChEBI:CHEBI:57599,
CC ChEBI:CHEBI:57666; EC=5.1.3.9; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01235};
CC -!- PATHWAY: Amino-sugar metabolism; N-acetylneuraminate degradation; D-
CC fructose 6-phosphate from N-acetylneuraminate: step 3/5.
CC {ECO:0000255|HAMAP-Rule:MF_01235}.
CC -!- SIMILARITY: Belongs to the NanE family. {ECO:0000255|HAMAP-
CC Rule:MF_01235}.
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DR EMBL; BA000018; BAB41531.1; -; Genomic_DNA.
DR PIR; H89796; H89796.
DR RefSeq; WP_000936718.1; NC_002745.2.
DR PDB; 1Y0E; X-ray; 1.95 A; A/B=1-222.
DR PDBsum; 1Y0E; -.
DR AlphaFoldDB; P65517; -.
DR SMR; P65517; -.
DR EnsemblBacteria; BAB41531; BAB41531; BAB41531.
DR KEGG; sau:SA0307; -.
DR HOGENOM; CLU_086300_1_0_9; -.
DR OMA; TRPMEIT; -.
DR UniPathway; UPA00629; UER00682.
DR EvolutionaryTrace; P65517; -.
DR Proteomes; UP000000751; Chromosome.
DR GO; GO:0047465; F:N-acylglucosamine-6-phosphate 2-epimerase activity; IEA:UniProtKB-EC.
DR GO; GO:0009385; F:N-acylmannosamine-6-phosphate 2-epimerase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006051; P:N-acetylmannosamine metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0019262; P:N-acetylneuraminate catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd04729; NanE; 1.
DR Gene3D; 3.20.20.70; -; 1.
DR HAMAP; MF_01235; ManNAc6P_epimer; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR007260; NanE.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR PANTHER; PTHR36204; PTHR36204; 1.
DR Pfam; PF04131; NanE; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Carbohydrate metabolism; Isomerase.
FT CHAIN 1..222
FT /note="Putative N-acetylmannosamine-6-phosphate 2-
FT epimerase"
FT /id="PRO_0000179799"
FT STRAND 4..9
FT /evidence="ECO:0007829|PDB:1Y0E"
FT HELIX 21..34
FT /evidence="ECO:0007829|PDB:1Y0E"
FT STRAND 37..43
FT /evidence="ECO:0007829|PDB:1Y0E"
FT HELIX 44..53
FT /evidence="ECO:0007829|PDB:1Y0E"
FT STRAND 58..61
FT /evidence="ECO:0007829|PDB:1Y0E"
FT HELIX 77..86
FT /evidence="ECO:0007829|PDB:1Y0E"
FT STRAND 89..94
FT /evidence="ECO:0007829|PDB:1Y0E"
FT STRAND 101..103
FT /evidence="ECO:0007829|PDB:1Y0E"
FT HELIX 105..115
FT /evidence="ECO:0007829|PDB:1Y0E"
FT STRAND 119..124
FT /evidence="ECO:0007829|PDB:1Y0E"
FT HELIX 128..136
FT /evidence="ECO:0007829|PDB:1Y0E"
FT STRAND 140..143
FT /evidence="ECO:0007829|PDB:1Y0E"
FT TURN 145..148
FT /evidence="ECO:0007829|PDB:1Y0E"
FT HELIX 160..172
FT /evidence="ECO:0007829|PDB:1Y0E"
FT STRAND 175..182
FT /evidence="ECO:0007829|PDB:1Y0E"
FT HELIX 186..194
FT /evidence="ECO:0007829|PDB:1Y0E"
FT STRAND 198..202
FT /evidence="ECO:0007829|PDB:1Y0E"
FT HELIX 204..207
FT /evidence="ECO:0007829|PDB:1Y0E"
FT HELIX 209..218
FT /evidence="ECO:0007829|PDB:1Y0E"
SQ SEQUENCE 222 AA; 24505 MW; 2977C9D2E628BD2D CRC64;
MLPHGLIVSC QALADEPLHS SFIMSKMALA AYEGGAVGIR ANTKEDILAI KETVDLPVIG
IVKRDYDHSD VFITATSKEV DELIESQCEV IALDATLQQR PKETLDELVS YIRTHAPNVE
IMADIATVEE AKNAARLGFD YIGTTLHGYT SYTQGQLLYQ NDFQFLKDVL QSVDAKVIAE
GNVITPDMYK RVMDLGVHCS VVGGAITRPK EITKRFVQVM ED
