ID   NANM_HAEI8              Reviewed;         375 AA.
AC   Q4QP39;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=N-acetylneuraminate epimerase {ECO:0000255|HAMAP-Rule:MF_01195};
DE            EC=5.1.3.24 {ECO:0000255|HAMAP-Rule:MF_01195};
DE   AltName: Full=N-acetylneuraminate mutarotase {ECO:0000255|HAMAP-Rule:MF_01195};
DE            Short=Neu5Ac mutarotase {ECO:0000255|HAMAP-Rule:MF_01195};
DE   AltName: Full=Sialic acid epimerase {ECO:0000255|HAMAP-Rule:MF_01195};
DE   Flags: Precursor;
GN   Name=nanM {ECO:0000255|HAMAP-Rule:MF_01195}; OrderedLocusNames=NTHI0235;
OS   Haemophilus influenzae (strain 86-028NP).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=281310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=86-028NP;
RX   PubMed=15968074; DOI=10.1128/jb.187.13.4627-4636.2005;
RA   Harrison A., Dyer D.W., Gillaspy A., Ray W.C., Mungur R., Carson M.B.,
RA   Zhong H., Gipson J., Gipson M., Johnson L.S., Lewis L., Bakaletz L.O.,
RA   Munson R.S. Jr.;
RT   "Genomic sequence of an otitis media isolate of nontypeable Haemophilus
RT   influenzae: comparative study with H. influenzae serotype d, strain KW20.";
RL   J. Bacteriol. 187:4627-4636(2005).
CC   -!- FUNCTION: Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-
CC       anomer, accelerating the equilibrium between the alpha- and beta-
CC       anomers. Probably facilitates sialidase-negative bacteria to compete
CC       sucessfully for limited amounts of extracellular Neu5Ac, which is
CC       likely taken up in the beta-anomer. In addition, the rapid removal of
CC       sialic acid from solution might be advantageous to the bacterium to
CC       damp down host responses. {ECO:0000255|HAMAP-Rule:MF_01195}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-acetyl-alpha-neuraminate = N-acetyl-beta-neuraminate;
CC         Xref=Rhea:RHEA:25233, ChEBI:CHEBI:58705, ChEBI:CHEBI:58770;
CC         EC=5.1.3.24; Evidence={ECO:0000255|HAMAP-Rule:MF_01195};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01195}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000255|HAMAP-Rule:MF_01195}.
CC   -!- SIMILARITY: Belongs to the NanM family. {ECO:0000255|HAMAP-
CC       Rule:MF_01195}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAX87208.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000057; AAX87208.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_041174728.1; NC_007146.2.
DR   AlphaFoldDB; Q4QP39; -.
DR   SMR; Q4QP39; -.
DR   EnsemblBacteria; AAX87208; AAX87208; NTHI0235.
DR   KEGG; hit:NTHI0235; -.
DR   HOGENOM; CLU_061535_0_0_6; -.
DR   Proteomes; UP000002525; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0016857; F:racemase and epimerase activity, acting on carbohydrates and derivatives; IEA:UniProtKB-UniRule.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 2.120.10.80; -; 2.
DR   HAMAP; MF_01195; NanM; 1.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR019936; Mutatrotase_YjhT-like.
DR   Pfam; PF01344; Kelch_1; 1.
DR   SUPFAM; SSF117281; SSF117281; 1.
DR   TIGRFAMs; TIGR03547; muta_rot_YjhT; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Isomerase; Kelch repeat; Periplasm; Repeat;
KW   Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01195"
FT   CHAIN           23..375
FT                   /note="N-acetylneuraminate epimerase"
FT                   /id="PRO_0000333061"
FT   REPEAT          43..87
FT                   /note="Kelch 1"
FT   REPEAT          89..140
FT                   /note="Kelch 2"
FT   REPEAT          142..176
FT                   /note="Kelch 3"
FT   REPEAT          177..222
FT                   /note="Kelch 4"
FT   REPEAT          225..273
FT                   /note="Kelch 5"
FT   REPEAT          295..344
FT                   /note="Kelch 6"
FT   REPEAT          346..375
FT                   /note="Kelch 7"
FT   ACT_SITE        231
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01195"
SQ   SEQUENCE   375 AA;  40283 MW;  43300C444D9487E8 CRC64;
     MKLTKTALCT ALFATFTFSA NAQTYPDLPV GIKGGTGALI GDTVYVGLGS GGDKFYTLDL
     KDPSAQWKEI ATFPGGERNQ PVAAAVDGKL YVFGGLQKNE KGELQLVNDA YRYNPSDNTW
     MKLPTRSPRG LVGSSGASHG DKVYILGGSN LSIFNGFFQD NVAAGEDKGK KDEIVAAYFD
     QRPEDYFFTT ELLSYEPSTN KWRNEGRIPF SGRAGAAFTI QGNDLVVVNG EIKPGLRTAE
     THQGKFTAKG VQWKNLPDLP APKGKSQDGL AGALAGYSNG YYLVTGGANF PGSIKQFKEG
     KLHAHKGLSK AWHNEVYTLN NGKWSIVGEL PMNIGYGFSV SYNNKVLLIG GETDGGKALT
     SVKAISYDGK KLTVE