NANM_SALTY
ID NANM_SALTY Reviewed; 386 AA.
AC Q8ZQ34;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=N-acetylneuraminate epimerase {ECO:0000255|HAMAP-Rule:MF_01195};
DE EC=5.1.3.24 {ECO:0000255|HAMAP-Rule:MF_01195};
DE AltName: Full=N-acetylneuraminate mutarotase {ECO:0000255|HAMAP-Rule:MF_01195};
DE Short=Neu5Ac mutarotase {ECO:0000255|HAMAP-Rule:MF_01195};
DE AltName: Full=Sialic acid epimerase {ECO:0000255|HAMAP-Rule:MF_01195};
DE Flags: Precursor;
GN Name=nanM {ECO:0000255|HAMAP-Rule:MF_01195}; OrderedLocusNames=STM1130;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
CC -!- FUNCTION: Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-
CC anomer, accelerating the equilibrium between the alpha- and beta-
CC anomers. Probably facilitates sialidase-negative bacteria to compete
CC sucessfully for limited amounts of extracellular Neu5Ac, which is
CC likely taken up in the beta-anomer. In addition, the rapid removal of
CC sialic acid from solution might be advantageous to the bacterium to
CC damp down host responses. {ECO:0000255|HAMAP-Rule:MF_01195}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetyl-alpha-neuraminate = N-acetyl-beta-neuraminate;
CC Xref=Rhea:RHEA:25233, ChEBI:CHEBI:58705, ChEBI:CHEBI:58770;
CC EC=5.1.3.24; Evidence={ECO:0000255|HAMAP-Rule:MF_01195};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01195}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000255|HAMAP-Rule:MF_01195}.
CC -!- SIMILARITY: Belongs to the NanM family. {ECO:0000255|HAMAP-
CC Rule:MF_01195}.
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DR EMBL; AE006468; AAL20061.1; -; Genomic_DNA.
DR RefSeq; NP_460102.1; NC_003197.2.
DR RefSeq; WP_000525751.1; NC_003197.2.
DR AlphaFoldDB; Q8ZQ34; -.
DR SMR; Q8ZQ34; -.
DR STRING; 99287.STM1130; -.
DR PaxDb; Q8ZQ34; -.
DR EnsemblBacteria; AAL20061; AAL20061; STM1130.
DR GeneID; 1252648; -.
DR KEGG; stm:STM1130; -.
DR PATRIC; fig|99287.12.peg.1197; -.
DR HOGENOM; CLU_061535_0_0_6; -.
DR OMA; PSTNKWR; -.
DR PhylomeDB; Q8ZQ34; -.
DR BioCyc; SENT99287:STM1130-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0016857; F:racemase and epimerase activity, acting on carbohydrates and derivatives; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.120.10.80; -; 2.
DR HAMAP; MF_01195; NanM; 1.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR019936; Mutatrotase_YjhT-like.
DR SUPFAM; SSF117281; SSF117281; 1.
DR TIGRFAMs; TIGR03547; muta_rot_YjhT; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Isomerase; Kelch repeat; Periplasm;
KW Reference proteome; Repeat; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01195"
FT CHAIN 30..386
FT /note="N-acetylneuraminate epimerase"
FT /id="PRO_0000333068"
FT REPEAT 51..95
FT /note="Kelch 1"
FT REPEAT 97..149
FT /note="Kelch 2"
FT REPEAT 151..186
FT /note="Kelch 3"
FT REPEAT 187..232
FT /note="Kelch 4"
FT REPEAT 235..284
FT /note="Kelch 5"
FT REPEAT 306..355
FT /note="Kelch 6"
FT REPEAT 357..386
FT /note="Kelch 7"
FT ACT_SITE 241
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01195"
SQ SEQUENCE 386 AA; 42491 MW; 7574B01C6AF3FA4A CRC64;
MGMQMKNFKK MMTLMALCFS VAITTSGYAT TLPDIPEPLK NGTGAIDNNG VIYVGLGTAG
TSWYKIDLKK QHKDWERIKS FPGGAREQSV SVFLNDELYV FGGVGKKNSE SPLQVYSDVY
KYSPVKNTWQ KVDTISPVGL TGHTGVKLNE TMVLITGGVN EHIFDKYFID IAAAAADESE
KNKVIYNYFN KPAKDYFFNK IVFIYNAKEN TWKNAGELPG AGTAGSSSVM GNNFLMLING
ELKPGLRTDV IYRAMWDNDK LTWLKNSQLP PSPGEQQQEG LAGAFSGYSH GVLLVGGGAN
FPGAKQNYTN GKFYSHEGIN KKWRDEVYGL INGHWQYMGK MKQPLGYGVS VSYGDEVFLI
GGENAKGKPV SSVTSFTMRD GNLLIK
