NANM_VIBC3
ID NANM_VIBC3 Reviewed; 384 AA.
AC A5F7B3; C3M1G8;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=N-acetylneuraminate epimerase {ECO:0000255|HAMAP-Rule:MF_01195};
DE EC=5.1.3.24 {ECO:0000255|HAMAP-Rule:MF_01195};
DE AltName: Full=N-acetylneuraminate mutarotase {ECO:0000255|HAMAP-Rule:MF_01195};
DE Short=Neu5Ac mutarotase {ECO:0000255|HAMAP-Rule:MF_01195};
DE AltName: Full=Sialic acid epimerase {ECO:0000255|HAMAP-Rule:MF_01195};
DE Flags: Precursor;
GN Name=nanM {ECO:0000255|HAMAP-Rule:MF_01195};
GN OrderedLocusNames=VC0395_A1372, VC395_1888;
OS Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS O395).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=345073;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA Heidelberg J.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
CC -!- FUNCTION: Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-
CC anomer, accelerating the equilibrium between the alpha- and beta-
CC anomers. Probably facilitates sialidase-negative bacteria to compete
CC sucessfully for limited amounts of extracellular Neu5Ac, which is
CC likely taken up in the beta-anomer. In addition, the rapid removal of
CC sialic acid from solution might be advantageous to the bacterium to
CC damp down host responses. {ECO:0000255|HAMAP-Rule:MF_01195}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetyl-alpha-neuraminate = N-acetyl-beta-neuraminate;
CC Xref=Rhea:RHEA:25233, ChEBI:CHEBI:58705, ChEBI:CHEBI:58770;
CC EC=5.1.3.24; Evidence={ECO:0000255|HAMAP-Rule:MF_01195};
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01195}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000255|HAMAP-Rule:MF_01195}.
CC -!- SIMILARITY: Belongs to the NanM family. {ECO:0000255|HAMAP-
CC Rule:MF_01195}.
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DR EMBL; CP000627; ABQ20169.1; -; Genomic_DNA.
DR EMBL; CP001235; ACP09884.1; -; Genomic_DNA.
DR RefSeq; WP_001056548.1; NZ_JAACZH010000016.1.
DR AlphaFoldDB; A5F7B3; -.
DR SMR; A5F7B3; -.
DR STRING; 345073.VC395_1888; -.
DR EnsemblBacteria; ABQ20169; ABQ20169; VC0395_A1372.
DR GeneID; 57740418; -.
DR KEGG; vco:VC0395_A1372; -.
DR KEGG; vcr:VC395_1888; -.
DR PATRIC; fig|345073.21.peg.1829; -.
DR eggNOG; COG3055; Bacteria.
DR HOGENOM; CLU_061535_0_0_6; -.
DR OMA; PSTNKWR; -.
DR Proteomes; UP000000249; Chromosome 2.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0016857; F:racemase and epimerase activity, acting on carbohydrates and derivatives; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 2.120.10.80; -; 2.
DR HAMAP; MF_01195; NanM; 1.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR019936; Mutatrotase_YjhT-like.
DR Pfam; PF01344; Kelch_1; 1.
DR SUPFAM; SSF117281; SSF117281; 1.
DR TIGRFAMs; TIGR03547; muta_rot_YjhT; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism; Isomerase; Kelch repeat; Periplasm; Repeat;
KW Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01195"
FT CHAIN 25..384
FT /note="N-acetylneuraminate epimerase"
FT /id="PRO_0000333073"
FT REPEAT 46..90
FT /note="Kelch 1"
FT REPEAT 92..145
FT /note="Kelch 2"
FT REPEAT 147..184
FT /note="Kelch 3"
FT REPEAT 185..230
FT /note="Kelch 4"
FT REPEAT 233..281
FT /note="Kelch 5"
FT REPEAT 303..352
FT /note="Kelch 6"
FT REPEAT 354..383
FT /note="Kelch 7"
FT ACT_SITE 239
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01195"
SQ SEQUENCE 384 AA; 41944 MW; 513B179249784BE6 CRC64;
MNMKTLLTYA TLLSVTAFSH VVYADNQWPD LPTGFKDGVG AQVGSKVYVG LGSLGKSFYV
LDLNALSKGW QKIADFTGAE RSGATASVIG NYIYLFGGSG KAEPSDPSPI LFDSVYRYDT
KKDSWEKMNT TSPVGLLGAS SYSPDNRQIL FFGGYNKAYF DRYLRDISTT DKQVNPEVWQ
RIVDDYMGMT PTDYKWNRNV ISYLPEKQEW RDLGVSTYLP NCGSATVIEG NKVTLISGEI
KPGLRTAEVK QYEFGMDQPW KSLLPLPAPQ TSNIQEGVAG AFSGKTNGVV VVAGGANFHG
AKQAFENGKM FAHEGLPKAF NSEIYVEKEG IWSTVNSLPE GLAYGASFTT SEGVLIVGGE
KSGKEMSHKV YMLAWNGSTV EVID
