NANOG_BOVIN
ID NANOG_BOVIN Reviewed; 300 AA.
AC Q4JM65;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Homeobox protein NANOG;
DE AltName: Full=Homeobox transcription factor Nanog;
GN Name=NANOG;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Blastocyst;
RA Kocabas A.M., Beyhan Z., Cibelli J.B.;
RT "Molecular cloning and characterization of bovine Nanog cDNA and its mRNA
RT expression during preimplantation development of in vitro fertilized and
RT nuclear transfer embryos.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription regulator involved in inner cell mass and
CC embryonic stem (ES) cells proliferation and self-renewal. Imposes
CC pluripotency on ES cells and prevents their differentiation towards
CC extraembryonic endoderm and trophectoderm lineages. Blocks bone
CC morphogenetic protein-induced mesoderm differentiation of ES cells by
CC physically interacting with SMAD1 and interfering with the recruitment
CC of coactivators to the active SMAD transcriptional complexes. Acts as a
CC transcriptional activator and repressor. Binds optimally to the DNA
CC consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-
CC 3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its
CC expression in differentiating (ES) cells: binds to its own promoter
CC following interaction with ZNF281/ZFP281, leading to recruitment of the
CC NuRD complex and subsequent repression of expression. When
CC overexpressed, promotes cells to enter into S phase and proliferation
CC (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:Q80Z64,
CC ECO:0000250|UniProtKB:Q9H9S0}.
CC -!- SUBUNIT: Interacts with SMAD1. Interacts with SALL4. Interacts with
CC ZNF281/ZFP281 (By similarity). Interacts with PCGF1 (By similarity).
CC Interacts with ESRRB; reciprocally modulates their transcriptional
CC activities. Interacts with NSD2 (By similarity).
CC {ECO:0000250|UniProtKB:Q80Z64, ECO:0000250|UniProtKB:Q9H9S0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- SIMILARITY: Belongs to the Nanog homeobox family. {ECO:0000305}.
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DR EMBL; DQ069776; AAY84556.1; -; mRNA.
DR RefSeq; NP_001020515.1; NM_001025344.1.
DR AlphaFoldDB; Q4JM65; -.
DR SMR; Q4JM65; -.
DR STRING; 9913.ENSBTAP00000027863; -.
DR PaxDb; Q4JM65; -.
DR Ensembl; ENSBTAT00000027863; ENSBTAP00000027863; ENSBTAG00000020916.
DR GeneID; 538951; -.
DR KEGG; bta:538951; -.
DR CTD; 79923; -.
DR VEuPathDB; HostDB:ENSBTAG00000020916; -.
DR eggNOG; KOG0491; Eukaryota.
DR GeneTree; ENSGT00670000098076; -.
DR HOGENOM; CLU_086240_0_0_1; -.
DR InParanoid; Q4JM65; -.
DR OMA; AWSNHSW; -.
DR OrthoDB; 1141558at2759; -.
DR TreeFam; TF337402; -.
DR Proteomes; UP000009136; Chromosome 5.
DR Bgee; ENSBTAG00000020916; Expressed in conceptus.
DR GO; GO:0000785; C:chromatin; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0003682; F:chromatin binding; IEA:Ensembl.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:Ensembl.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0070577; F:lysine-acetylated histone binding; IEA:Ensembl.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001010; F:RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity; IEA:Ensembl.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR GO; GO:0030509; P:BMP signaling pathway; IEA:Ensembl.
DR GO; GO:0043697; P:cell dedifferentiation; IEA:Ensembl.
DR GO; GO:0009880; P:embryonic pattern specification; IEA:Ensembl.
DR GO; GO:0010467; P:gene expression; IEA:Ensembl.
DR GO; GO:0008406; P:gonad development; IEA:Ensembl.
DR GO; GO:0001710; P:mesodermal cell fate commitment; IEA:Ensembl.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; IEA:Ensembl.
DR GO; GO:0010454; P:negative regulation of cell fate commitment; IEA:Ensembl.
DR GO; GO:2000737; P:negative regulation of stem cell differentiation; IEA:Ensembl.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:Ensembl.
DR GO; GO:0045931; P:positive regulation of mitotic cell cycle; IEA:Ensembl.
DR GO; GO:2000035; P:regulation of stem cell division; IEA:Ensembl.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0032526; P:response to retinoic acid; IEA:Ensembl.
DR GO; GO:0035019; P:somatic stem cell population maintenance; IEA:Ensembl.
DR GO; GO:0048863; P:stem cell differentiation; IEA:Ensembl.
DR GO; GO:0017145; P:stem cell division; IEA:Ensembl.
DR GO; GO:0019827; P:stem cell population maintenance; ISS:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..300
FT /note="Homeobox protein NANOG"
FT /id="PRO_0000261417"
FT REPEAT 190..194
FT /note="1"
FT REPEAT 195..199
FT /note="2"
FT REPEAT 200..204
FT /note="3"
FT REPEAT 205..209
FT /note="4"
FT REPEAT 210..214
FT /note="5"
FT REPEAT 215..219
FT /note="6"
FT REPEAT 220..224
FT /note="7"
FT REPEAT 225..229
FT /note="8"
FT REPEAT 230..234
FT /note="9"
FT REGION 1..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 117..146
FT /note="Required for DNA-binding"
FT /evidence="ECO:0000250|UniProtKB:Q9H9S0"
FT REGION 190..234
FT /note="9 X repeats starting with a Trp in each unit"
FT REGION 190..234
FT /note="Sufficient for transactivation activity"
FT /evidence="ECO:0000250"
FT REGION 235..300
FT /note="Sufficient for strong transactivation activity"
FT /evidence="ECO:0000250"
FT COMPBIAS 12..73
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 300 AA; 34332 MW; E135E334F558C693 CRC64;
MSVGPACPQS LLGPEASNSR ESSPMPEESY VSLQTSSADT LDTDTVSPLP SSMDLLIQDS
PDSSTSPRVK PLSPSVEEST EKEETVPVKK QKIRTVFSQT QLCVLNDRFQ RQKYLSLQQM
QELSNILNLS YKQVKTWFQN QRMKCKKWQK NNWPRNSNGM PQGPAMAEYP GFYSYHQGCL
VNSPGNLPMW GNQTWNNPTW SNQSWNSQSW SNHSWNSQAW CPQAWNNQPW NNQFNNYMEE
FLQPGIQLQQ NSPVCDLEAT LGTAGENYNV IQQTVKYFNS QQQITDLFPN YPLNIQPEDL
