NANOG_MACFA
ID NANOG_MACFA Reviewed; 305 AA.
AC Q5TM84;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Homeobox protein NANOG;
DE AltName: Full=Homeobox transcription factor Nanog;
GN Name=NANOG; Synonyms=STM1;
OS Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9541;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryonic stem cell;
RX PubMed=15582778; DOI=10.1016/j.mod.2004.08.008;
RA Hatano S.Y., Tada M., Kimura H., Yamaguchi S., Kono T., Nakano T.,
RA Suemori H., Nakatsuji N., Tada T.;
RT "Pluripotential competence of cells associated with Nanog activity.";
RL Mech. Dev. 122:67-79(2005).
CC -!- FUNCTION: Transcription regulator involved in inner cell mass and
CC embryonic stem (ES) cells proliferation and self-renewal. Imposes
CC pluripotency on ES cells and prevents their differentiation towards
CC extraembryonic endoderm and trophectoderm lineages. Blocks bone
CC morphogenetic protein-induced mesoderm differentiation of ES cells by
CC physically interacting with SMAD1 and interfering with the recruitment
CC of coactivators to the active SMAD transcriptional complexes. Acts as a
CC transcriptional activator or repressor. Binds optimally to the DNA
CC consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-
CC 3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its
CC expression in differentiating (ES) cells: binds to its own promoter
CC following interaction with ZNF281/ZFP281, leading to recruitment of the
CC NuRD complex and subsequent repression of expression. When
CC overexpressed, promotes cells to enter into S phase and proliferation
CC (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:Q80Z64,
CC ECO:0000250|UniProtKB:Q9H9S0}.
CC -!- SUBUNIT: Interacts with SMAD1. Interacts with SALL4. Interacts with
CC ZNF281/ZFP281 (By similarity). Interacts with PCGF1 (By similarity).
CC Interacts with ESRRB; reciprocally modulates their transcriptional
CC activities. Interacts with NSD2 (By similarity).
CC {ECO:0000250|UniProtKB:Q80Z64, ECO:0000250|UniProtKB:Q9H9S0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryonic stem (ES) cells.
CC {ECO:0000269|PubMed:15582778}.
CC -!- SIMILARITY: Belongs to the Nanog homeobox family. {ECO:0000305}.
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DR EMBL; AB126938; BAD72891.1; -; mRNA.
DR RefSeq; NP_001274577.1; NM_001287648.1.
DR AlphaFoldDB; Q5TM84; -.
DR SMR; Q5TM84; -.
DR STRING; 9541.XP_005570081.1; -.
DR Ensembl; ENSMFAT00000035085; ENSMFAP00000020930; ENSMFAG00000033399.
DR GeneID; 102114964; -.
DR CTD; 79923; -.
DR eggNOG; KOG0491; Eukaryota.
DR GeneTree; ENSGT00670000098076; -.
DR OrthoDB; 1141558at2759; -.
DR Proteomes; UP000233100; Chromosome 11.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; ISS:UniProtKB.
DR GO; GO:0019827; P:stem cell population maintenance; ISS:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..305
FT /note="Homeobox protein NANOG"
FT /id="PRO_0000261421"
FT REPEAT 196..200
FT /note="1"
FT REPEAT 201..205
FT /note="2"
FT REPEAT 206..210
FT /note="3"
FT REPEAT 216..220
FT /note="4"
FT REPEAT 221..225
FT /note="5"
FT REPEAT 226..230
FT /note="6"
FT REPEAT 231..235
FT /note="7"
FT REPEAT 236..240
FT /note="8"
FT REGION 1..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 122..151
FT /note="Required for DNA-binding"
FT /evidence="ECO:0000250|UniProtKB:Q9H9S0"
FT REGION 196..240
FT /note="8 X repeats starting with a Trp in each unit"
FT REGION 196..240
FT /note="Sufficient for transactivation activity"
FT /evidence="ECO:0000250"
FT REGION 241..305
FT /note="Sufficient for strong transactivation activity"
FT /evidence="ECO:0000250"
FT COMPBIAS 35..81
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 305 AA; 34153 MW; 3FC6FD226DF5E998 CRC64;
MSVDPACPQS LPCLEASDSK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL
LIQDSPDSST SPKGKQPTAA ENSATKKEDK VPVKKQKART VFSSAQLCVL NDRFQRQKYL
SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS
CHQGCLVNPT GNLPMWSNQT WNNSSWSNQT QNIQSWSNHS WNAQTWCTQS WNNQAWNSPF
SNCGEESLQS CLQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYLSTPQTV DLLLNYSTNM
QPEDV
