NANOG_MUSMM
ID NANOG_MUSMM Reviewed; 305 AA.
AC Q5TM83;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Homeobox protein NANOG;
DE AltName: Full=Homeobox transcription factor Nanog;
GN Name=Nanog; Synonyms=Stm1;
OS Mus musculus molossinus (Japanese house mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=57486;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=15582778; DOI=10.1016/j.mod.2004.08.008;
RA Hatano S.Y., Tada M., Kimura H., Yamaguchi S., Kono T., Nakano T.,
RA Suemori H., Nakatsuji N., Tada T.;
RT "Pluripotential competence of cells associated with Nanog activity.";
RL Mech. Dev. 122:67-79(2005).
CC -!- FUNCTION: Transcription regulator involved in inner cell mass and
CC embryonic stem (ES) cells proliferation and self-renewal. Imposes
CC pluripotency on ES cells and prevents their differentiation towards
CC extraembryonic endoderm and trophectoderm lineages. Blocks bone
CC morphogenetic protein-induced mesoderm differentiation of ES cells by
CC physically interacting with SMAD1 and interfering with the recruitment
CC of coactivators to the active SMAD transcriptional complexes. Acts as a
CC transcriptional activator or repressor. Binds optimally to the DNA
CC consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-
CC 3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its
CC expression in differentiating (ES) cells: binds to its own promoter
CC following interaction with ZNF281/ZFP281, leading to recruitment of the
CC NuRD complex and subsequent repression of expression. When
CC overexpressed, promotes cells to enter into S phase and proliferation
CC (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:Q80Z64,
CC ECO:0000250|UniProtKB:Q9H9S0}.
CC -!- SUBUNIT: Interacts with SMAD1. Interacts with SALL4. Interacts with
CC ZNF281/ZFP281 (By similarity). Interacts with PCGF1 (By similarity).
CC Interacts with ESRRB; reciprocally modulates their transcriptional
CC activities. Interacts with NSD2 (By similarity).
CC {ECO:0000250|UniProtKB:Q80Z64, ECO:0000250|UniProtKB:Q9H9S0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- SIMILARITY: Belongs to the Nanog homeobox family. {ECO:0000305}.
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DR EMBL; AB126939; BAD72892.1; -; mRNA.
DR AlphaFoldDB; Q5TM83; -.
DR SMR; Q5TM83; -.
DR PRIDE; Q5TM83; -.
DR MGI; MGI:1919200; Nanog.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Homeobox; Nucleus; Repeat;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..305
FT /note="Homeobox protein NANOG"
FT /id="PRO_0000261420"
FT REPEAT 198..202
FT /note="1"
FT REPEAT 203..207
FT /note="2"
FT REPEAT 208..212
FT /note="3"
FT REPEAT 213..217
FT /note="4"
FT REPEAT 218..222
FT /note="5"
FT REPEAT 223..227
FT /note="6"
FT REPEAT 228..232
FT /note="7"
FT REPEAT 233..237
FT /note="8"
FT REPEAT 238..242
FT /note="9"
FT REPEAT 243..247
FT /note="10"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 46..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 123..152
FT /note="Required for DNA-binding"
FT /evidence="ECO:0000250|UniProtKB:Q9H9S0"
FT REGION 198..247
FT /note="10 X repeats starting with a Trp in each unit"
FT REGION 198..247
FT /note="Sufficient for transactivation activity"
FT /evidence="ECO:0000250"
FT REGION 248..305
FT /note="Sufficient for strong transactivation activity"
FT /evidence="ECO:0000250"
FT COMPBIAS 12..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..77
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 79..95
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 305 AA; 34194 MW; 7F297508B77EEF20 CRC64;
MSVGLPGPHS LPSSEEASNS GNASSMPAVF HPENYSCLQG SATEMLCTEA ASPRPSSEDL
PLQGSPDSST SPKQKLSSPE ADKGPEEEEN KVLARKQKMR TVFSQAQLCA LKDRFQKQKY
LSLQQMQELS SILNLSYKQV KTWFQNQRMK CKRWQKNQWL KTSNGLIQKG SAPVEYPSIH
CSYPQGYLVN ASGSLSMWGS QTWTNPTWSS QTWTNPTWNN QTWTNPTWSS QAWTAQSWNG
QPWNAAPLHN FGEDFLQPYI QLQQNSSASD LEVNLEATRE SHAHFSTPQA LELFLNYSVT
PPGEI
