NANT_ALIF1
ID NANT_ALIF1 Reviewed; 505 AA.
AC Q5E733;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=N-acetylneuraminate transporter {ECO:0000305};
GN Name=nanT {ECO:0000312|EMBL:AAW85163.1};
GN OrderedLocusNames=VF_0668 {ECO:0000312|EMBL:AAW85163.1};
OS Aliivibrio fischeri (strain ATCC 700601 / ES114) (Vibrio fischeri).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Aliivibrio.
OX NCBI_TaxID=312309;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700601 / ES114;
RX PubMed=15703294; DOI=10.1073/pnas.0409900102;
RA Ruby E.G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R.,
RA Lostroh P., Lupp C., McCann J., Millikan D., Schaefer A., Stabb E.,
RA Stevens A., Visick K., Whistler C., Greenberg E.P.;
RT "Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with
RT pathogenic congeners.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:3004-3009(2005).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700601 / ES114;
RX PubMed=28446608; DOI=10.1074/jbc.m116.763193;
RA Pan S., Nikolakakis K., Adamczyk P.A., Pan M., Ruby E.G., Reed J.L.;
RT "Model-enabled gene search (MEGS) allows fast and direct discovery of
RT enzymatic and transport gene functions in the marine bacterium Vibrio
RT fischeri.";
RL J. Biol. Chem. 292:10250-10261(2017).
CC -!- FUNCTION: Seems to be involved in N-acetylneuraminate transport.
CC Complements an E. coli nanT deletion mutant.
CC {ECO:0000269|PubMed:28446608}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant cannot grow in minimal medium.
CC Mutant fails to persist in the squid. {ECO:0000269|PubMed:28446608}.
CC -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC family. {ECO:0000305}.
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DR EMBL; CP000020; AAW85163.1; -; Genomic_DNA.
DR RefSeq; WP_011261398.1; NC_006840.2.
DR RefSeq; YP_204051.1; NC_006840.2.
DR AlphaFoldDB; Q5E733; -.
DR SMR; Q5E733; -.
DR STRING; 312309.VF_0668; -.
DR TCDB; 2.A.21.3.7; the solute:sodium symporter (sss) family.
DR EnsemblBacteria; AAW85163; AAW85163; VF_0668.
DR KEGG; vfi:VF_0668; -.
DR PATRIC; fig|312309.11.peg.661; -.
DR eggNOG; COG0591; Bacteria.
DR HOGENOM; CLU_018808_11_4_6; -.
DR OMA; YVFIHFI; -.
DR OrthoDB; 231452at2; -.
DR Proteomes; UP000000537; Chromosome I.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1730.10; -; 1.
DR InterPro; IPR038377; Na/Glc_symporter_sf.
DR InterPro; IPR001734; Na/solute_symporter.
DR Pfam; PF00474; SSF; 1.
DR TIGRFAMs; TIGR00813; sss; 1.
DR PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..505
FT /note="N-acetylneuraminate transporter"
FT /id="PRO_0000440876"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 80..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 505 AA; 55564 MW; 96B49D9DAE1BA6D3 CRC64;
MEAQSFGTLN YIALFAYLGA IMAVGVYFAR RQKSADDYFK AGGRIPGWAA GFSVFATTLS
SITFMSIPAK AYTSDWTFLI GQYVAIAILP IVFWFYIPFF RKLNLTSVYE YLERRFDVRM
RLFGSISFML FHIGRIAIVT YLTALALMPF IDISPLMIVF LIGVLCIIYT FLGGIEGVIW
TDVIQGVMLS VAAILIFVVI CFNVDGGIVE VFSMSNQADK YFPAEQFSWS WTDSTIPVLM
IGFFFASLQQ FTASQDVVQR YIVTDNIDET KKALITNAKL VACVPIFFFA VGSALFAYYT
QNPELLPENF NTGGILPFYV ISQMPVGVAG LIIAAIFAAS QSSISSSLNS IAACFTSDIY
EKVSKNPTSE QKLRIGRTLT VVAGLLGVVA STYLIMSNES EIWDAFNSLL GLMGGPMTGL
FMLGIFVRRA NANSALLGVV ASIATVLWVR SATDLNFFFY GVIGTLMVVI VGYLTAPMFK
NNLNSDEIDE LSATKEKRST TAEKA
