ID   NANT_ECO5E              Reviewed;         496 AA.
AC   B5YSV1;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-NOV-2008, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Sialic acid transporter NanT {ECO:0000255|HAMAP-Rule:MF_01238};
DE   AltName: Full=Sialic acid permease {ECO:0000255|HAMAP-Rule:MF_01238};
DE   AltName: Full=Sialic acid/H(+) symporter {ECO:0000255|HAMAP-Rule:MF_01238};
GN   Name=nanT {ECO:0000255|HAMAP-Rule:MF_01238};
GN   OrderedLocusNames=ECH74115_4541;
OS   Escherichia coli O157:H7 (strain EC4115 / EHEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=444450;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EC4115 / EHEC;
RX   PubMed=22135463; DOI=10.1073/pnas.1107176108;
RA   Eppinger M., Mammel M.K., Leclerc J.E., Ravel J., Cebula T.A.;
RT   "Genomic anatomy of Escherichia coli O157:H7 outbreaks.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20142-20147(2011).
CC   -!- FUNCTION: Catalyzes the proton-dependent transport of sialic acid.
CC       {ECO:0000255|HAMAP-Rule:MF_01238}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+)(in) + N-acetylneuraminate(in) = H(+)(out) + N-
CC         acetylneuraminate(out); Xref=Rhea:RHEA:28987, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:35418; Evidence={ECO:0000255|HAMAP-Rule:MF_01238};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01238}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01238}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Sialate:H(+)
CC       symporter (SHS) (TC 2.A.1.12) family. {ECO:0000255|HAMAP-
CC       Rule:MF_01238}.
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DR   EMBL; CP001164; ACI36447.1; -; Genomic_DNA.
DR   RefSeq; WP_000108469.1; NC_011353.1.
DR   AlphaFoldDB; B5YSV1; -.
DR   KEGG; ecf:ECH74115_4541; -.
DR   HOGENOM; CLU_001265_46_8_6; -.
DR   OMA; HWLLLTY; -.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0015538; F:sialic acid:proton symporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   HAMAP; MF_01238; MFS_NanT; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR004742; SA_transporter.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00891; 2A0112; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Sugar transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..496
FT                   /note="Sialic acid transporter NanT"
FT                   /id="PRO_1000214043"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        224..244
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        247..267
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        278..298
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        313..333
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        353..375
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        406..426
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
FT   TRANSMEM        431..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01238"
SQ   SEQUENCE   496 AA;  53611 MW;  88121FE40ACF1211 CRC64;
     MSTTTQNIPW YRHLNRAQWR AFSAAWLGYL LDGFDFVLIA LVLTEVQGEF GLTTVQAASL
     ISAAFISRWF GGLMLGAMGD RYGRRLAMVT SIVLFSAGTL ACGFAPGYIT MFIARLVIGM
     GMAGEYGSSA TYVIESWPKH LRNKASGFLI SGFSVGAVVA AQVYSLVVPV WGWRALFFIG
     ILPIIFALWL RKNIPEAEDW KEKHAGKAPV RTMVDILYRG EHRIANIVMT LAAATALWFC
     FAGNLQNAAI VAVLGLLCTA IFISFMVQST GKRWPTGVML MVVVLFAFLY SWPIQALLPT
     YLKTDLAYNP HTVANVLFFS GFGAAVGCCV GGFLGDWLGT RKAYVCSLLA SQLLIIPVFA
     IGGANVWVLG LLLFFQQMLG QGIAGILPKL IGGYFDTDQR AAGLGFTYNV GALGGALAPI
     IGALIAQRLD LGTALASLSF SLTFVVILLI GLDMPSRVQR WLRPEALRTH DAIDGKPFSG
     AVPFGSAKND LVKTKS