NAP1D_ARATH
ID NAP1D_ARATH Reviewed; 317 AA.
AC F4JEI8; Q9LIC0;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 65.
DE RecName: Full=Nucleosome assembly protein 1;4;
DE Short=AtNAP1;4;
DE AltName: Full=Nucleosome/chromatin assembly factor group A4;
GN Name=NAP1;4; Synonyms=NFA4; OrderedLocusNames=At3g13782; ORFNames=MMM17.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP INTERACTION WITH HISTONE H2A.
RX PubMed=19228338; DOI=10.1111/j.1365-313x.2009.03844.x;
RA Liu Z., Zhu Y., Gao J., Yu F., Dong A., Shen W.H.;
RT "Molecular and reverse genetic characterization of NUCLEOSOME ASSEMBLY
RT PROTEIN1 (NAP1) genes unravels their function in transcription and
RT nucleotide excision repair in Arabidopsis thaliana.";
RL Plant J. 59:27-38(2009).
CC -!- FUNCTION: May modulate chromatin structure by regulation of nucleosome
CC assembly/disassembly. {ECO:0000250}.
CC -!- SUBUNIT: Can form homomeric and heteromeric protein complexes with
CC NAP1;1, NAP1;2 and NAP1;3. Binds histone H2A.
CC {ECO:0000269|PubMed:19228338}.
CC -!- INTERACTION:
CC F4JEI8; Q9SZI2: NAP1;1; NbExp=2; IntAct=EBI-6913300, EBI-4424361;
CC F4JEI8; Q9ZUP3: NAP1;2; NbExp=3; IntAct=EBI-6913300, EBI-1997989;
CC F4JEI8; Q94K07: NAP1;3; NbExp=3; IntAct=EBI-6913300, EBI-4430887;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19228338}. Cytoplasm
CC {ECO:0000269|PubMed:19228338}. Note=Shuttles between cytoplasm and
CC nucleus.
CC -!- TISSUE SPECIFICITY: Expressed in the root segment covering the apical
CC end of the differentiation zone, the elongation zone of the root and
CC the mature pollen within the anthers of open flowers.
CC {ECO:0000269|PubMed:19228338}.
CC -!- DOMAIN: The acidic domain is probably involved in the interaction with
CC histones.
CC -!- SIMILARITY: Belongs to the nucleosome assembly protein (NAP) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01928.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP001307; BAB01928.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75412.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63955.1; -; Genomic_DNA.
DR RefSeq; NP_001319544.1; NM_001338075.1.
DR RefSeq; NP_187993.2; NM_112230.3.
DR AlphaFoldDB; F4JEI8; -.
DR SMR; F4JEI8; -.
DR IntAct; F4JEI8; 3.
DR STRING; 3702.AT3G13782.1; -.
DR PaxDb; F4JEI8; -.
DR PRIDE; F4JEI8; -.
DR ProteomicsDB; 238430; -.
DR EnsemblPlants; AT3G13782.1; AT3G13782.1; AT3G13782.
DR EnsemblPlants; AT3G13782.2; AT3G13782.2; AT3G13782.
DR GeneID; 820589; -.
DR Gramene; AT3G13782.1; AT3G13782.1; AT3G13782.
DR Gramene; AT3G13782.2; AT3G13782.2; AT3G13782.
DR KEGG; ath:AT3G13782; -.
DR Araport; AT3G13782; -.
DR TAIR; locus:2091591; AT3G13782.
DR eggNOG; KOG1507; Eukaryota.
DR HOGENOM; CLU_038841_4_0_1; -.
DR InParanoid; F4JEI8; -.
DR OMA; YHVNDED; -.
DR OrthoDB; 1216172at2759; -.
DR PRO; PR:F4JEI8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4JEI8; baseline and differential.
DR Genevisible; F4JEI8; AT.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IDA:TAIR.
DR GO; GO:0042393; F:histone binding; IDA:UniProtKB.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IGI:TAIR.
DR GO; GO:0006334; P:nucleosome assembly; IBA:GO_Central.
DR InterPro; IPR037231; NAP-like_sf.
DR InterPro; IPR002164; NAP_family.
DR PANTHER; PTHR11875; PTHR11875; 1.
DR Pfam; PF00956; NAP; 1.
DR SUPFAM; SSF143113; SSF143113; 1.
PE 1: Evidence at protein level;
KW Chaperone; Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..317
FT /note="Nucleosome assembly protein 1;4"
FT /id="PRO_0000423682"
FT REGION 297..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 52..67
FT /note="Nuclear export signal"
FT /evidence="ECO:0000255"
FT MOTIF 214..219
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 298..317
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 317 AA; 36418 MW; FC86B7A67FE5CB0A CRC64;
MSNEENIKSD NKSGDSSDLP TIPALDIGAE ECDLLAELKN LTLKRPFDVK KLSPKVTKRV
LFLKDIQVTH DELEEKFLAE KSALEATYDN LYKPLFAKRY EIVNGVVEAE AEKEGVPNFW
LIAMKTNEML ANEITERDEA ALKYLKDIRS CRVEDTSRNF KLEFLFDSNL YFKNSVLSKT
YHVNDEDGPV LEKVIGTDIE WFPGKCLTHK VVVKKKTKKG PKKVNNIPMT KTENCESFFN
FFKPPEIPEI DEVDDYDDFD TIMTEELQNL MDQDYDIAVT IRDKLIPHAV SWFTGEALVD
EDDSDDNDDD DNDEKSD
