ID   NAPA_DICDI              Reviewed;        1160 AA.
AC   Q869Q3; Q555I6;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Nck-associated protein 1 homolog;
GN   Name=napA; Synonyms=nap1; ORFNames=DDB_G0274519;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   IDENTIFICATION IN THE WAVE COMPLEX, AND DISRUPTION PHENOTYPE.
RX   PubMed=16581519; DOI=10.1016/j.cub.2006.02.068;
RA   Ibarra N., Blagg S.L., Vazquez F., Insall R.H.;
RT   "Nap1 regulates Dictyostelium cell motility and adhesion through SCAR-
RT   dependent and -independent pathways.";
RL   Curr. Biol. 16:717-722(2006).
CC   -!- FUNCTION: Involved in regulation of actin and microtubule organization.
CC       Involved in cell adhesion.
CC   -!- SUBUNIT: Part of a Scar/WAVE complex containing brk1, scrA, abiA, pirA
CC       and napA. {ECO:0000269|PubMed:16581519}.
CC   -!- DISRUPTION PHENOTYPE: Cells have defects in motility, and various
CC       developmental defects, notably in aggregation and adhesion.
CC       {ECO:0000269|PubMed:16581519}.
CC   -!- SIMILARITY: Belongs to the HEM-1/HEM-2 family. {ECO:0000305}.
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DR   EMBL; AAFI02000012; EAL70152.1; -; Genomic_DNA.
DR   RefSeq; XP_644083.1; XM_638991.1.
DR   AlphaFoldDB; Q869Q3; -.
DR   SMR; Q869Q3; -.
DR   STRING; 44689.DDB0231423; -.
DR   PaxDb; Q869Q3; -.
DR   EnsemblProtists; EAL70152; EAL70152; DDB_G0274519.
DR   GeneID; 8619512; -.
DR   KEGG; ddi:DDB_G0274519; -.
DR   dictyBase; DDB_G0274519; napA.
DR   eggNOG; KOG1917; Eukaryota.
DR   HOGENOM; CLU_004450_1_0_1; -.
DR   InParanoid; Q869Q3; -.
DR   OMA; VGMVMYN; -.
DR   PhylomeDB; Q869Q3; -.
DR   Reactome; R-DDI-2029482; Regulation of actin dynamics for phagocytic cup formation.
DR   Reactome; R-DDI-5663213; RHO GTPases Activate WASPs and WAVEs.
DR   Reactome; R-DDI-6798695; Neutrophil degranulation.
DR   Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR   Reactome; R-DDI-9013404; RAC2 GTPase cycle.
DR   Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR   PRO; PR:Q869Q3; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0031252; C:cell leading edge; IC:dictyBase.
DR   GO; GO:0005911; C:cell-cell junction; IC:dictyBase.
DR   GO; GO:0032433; C:filopodium tip; IC:dictyBase.
DR   GO; GO:0031143; C:pseudopodium; IC:dictyBase.
DR   GO; GO:0031209; C:SCAR complex; IDA:dictyBase.
DR   GO; GO:0016477; P:cell migration; IBA:GO_Central.
DR   GO; GO:0000902; P:cell morphogenesis; IBA:GO_Central.
DR   GO; GO:0048870; P:cell motility; IMP:dictyBase.
DR   GO; GO:0030031; P:cell projection assembly; IBA:GO_Central.
DR   GO; GO:0098609; P:cell-cell adhesion; IMP:dictyBase.
DR   GO; GO:0031589; P:cell-substrate adhesion; IMP:dictyBase.
DR   GO; GO:0030866; P:cortical actin cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0046847; P:filopodium assembly; IMP:dictyBase.
DR   GO; GO:0006972; P:hyperosmotic response; IEP:dictyBase.
DR   GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR   GO; GO:0110094; P:polyphosphate-mediated signaling; IMP:dictyBase.
DR   GO; GO:0046956; P:positive phototaxis; IMP:dictyBase.
DR   GO; GO:0030838; P:positive regulation of actin filament polymerization; IMP:dictyBase.
DR   GO; GO:0008064; P:regulation of actin polymerization or depolymerization; TAS:dictyBase.
DR   GO; GO:0097178; P:ruffle assembly; IMP:dictyBase.
DR   InterPro; IPR019137; Nck-associated_protein-1.
DR   PANTHER; PTHR12093; PTHR12093; 1.
DR   Pfam; PF09735; Nckap1; 1.
PE   1: Evidence at protein level;
KW   Cell adhesion; Reference proteome.
FT   CHAIN           1..1160
FT                   /note="Nck-associated protein 1 homolog"
FT                   /id="PRO_0000331389"
SQ   SEQUENCE   1160 AA;  133540 MW;  89F7D2B447919C0A CRC64;
     MAHTNLPEKF QVVIENGENI LQRVYNTYKL FQNNKVKPSF FNDEKISKIL KNLIAKYPEL
     PENNDKSITG FDLLTSRAKQ HLEELEDHYY TITDAYDWKE ASFILMQEIS SNTVSINFNN
     NIQLCSKFLD VLVLYGKINY LVSLIPDKKI ITAVYAKLFL YTRSASEPTF SKMGRWINDI
     EQPFKKIQEE FRVLNDAVGH ALSSFELTYA KRRVITQLRK DGALNLILKP EDIARPVQDS
     YRIELAYAGR IQQWILFGYL FAPGTLSTPQ SIELLRFTLS ECFYLSVFKD ISISIHNEFN
     TLFKNYKSKT INLQKQKKII KDAAQASTQE APRKHAERRV YIRQELEAMW NLFRDKPCLL
     APKINVLLAA LSMAKEEIFW YFRHTDVIPP EKVKKFYNKQ NEVREKRISS LLSLVDHLVQ
     LVHTHKKMIQ NYYLEYISGA DILGLQKVIT PQLLQNAGSI VTQAVNTIVN ELKSLNANGQ
     DYSFEGFRAN WMRLGYLLQS NSCPLKESES KQITSRLNLI YTHSKNVDCL DQLLDEYGNM
     TQLWSYKEPL FHSFDAAIVD MTCDQPSHSM IYLKLLSQFP NHVANQFYPE EKELIGKECV
     ELANSCLTKI TNRIVSIMAN TIASTFLSDE QQLADVNAAF PLLQKKKDWK PPKDFVPPIE
     PASESQFRNR ANLEQLRSEE KNAFQLCTAL NEFLDITIYD HIFVPREFLR EKLGSALKQY
     MRQSIQPPAP TSSSSQIDIN ITRLSTYESQ LRVFIGVLIL VENHVDIDIG DLIRETILTE
     FYAKALGKSG RVDWFPEGEI EMNELTLHSI TSYYVDLVSK KLNTPGVVFS PVKLGFISKA
     GTPFRAEEHA DLTEMRALCD LVGPYGIKVI EREILRFILT TTTSMKEILS LNAANLEEFA
     SNYYKPKAME LLKKFKTTDL DLIVTKSIAI GNALHLRSMI RESMKDVITD NFPYINNAVA
     NAFDQYNRNT FMFPDFLGVD TLALDSGLNV GIADQYLKVI LRKVSSEADK RIWELLPVMF
     SLTFYGNIWK ETQYKATIDA HSNNVHVLSK TIIDLLIAFG AINSTTGNEA ELFQSFKRFL
     EISSVNILRM FKGKQGEKFV PNEIQSVIIF LDKFTQQCPL LSKDSLEQYI PYSLIRNMYK
     DLYEHKNLQK QQSETSEQNF