NAP_BACSU
ID NAP_BACSU Reviewed; 300 AA.
AC P96688; Q797G3;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Uncharacterized carboxylesterase nap;
DE EC=3.1.1.1;
GN Name=nap; OrderedLocusNames=BSU05440;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT subtilis genome.";
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.96 ANGSTROMS) OF 1-294 IN COMPLEX WITH TRIETHYLENE
RP GLYCOL, AND SUBUNIT.
RG Joint center for structural genomics (JCSG);
RT "Crystal structure of putative hydrolase (2632844) from Bacillus subtilis
RT at 1.96 A resolution.";
RL Submitted (SEP-2007) to the PDB data bank.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+);
CC Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1;
CC -!- SUBUNIT: Monomer. {ECO:0000269|Ref.3}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. {ECO:0000305}.
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DR EMBL; AB001488; BAA19378.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12351.1; -; Genomic_DNA.
DR PIR; C69664; C69664.
DR RefSeq; NP_388425.1; NC_000964.3.
DR RefSeq; WP_003242638.1; NZ_JNCM01000031.1.
DR PDB; 2R11; X-ray; 1.96 A; A/B/C/D=1-294.
DR PDBsum; 2R11; -.
DR AlphaFoldDB; P96688; -.
DR SMR; P96688; -.
DR STRING; 224308.BSU05440; -.
DR ESTHER; bacsu-cbxnp; 6_AlphaBeta_hydrolase.
DR PaxDb; P96688; -.
DR PRIDE; P96688; -.
DR DNASU; 938069; -.
DR EnsemblBacteria; CAB12351; CAB12351; BSU_05440.
DR GeneID; 938069; -.
DR KEGG; bsu:BSU05440; -.
DR PATRIC; fig|224308.179.peg.583; -.
DR eggNOG; COG0596; Bacteria.
DR InParanoid; P96688; -.
DR OMA; SSTMWYP; -.
DR PhylomeDB; P96688; -.
DR BioCyc; BSUB:BSU05440-MON; -.
DR EvolutionaryTrace; P96688; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0080030; F:methyl indole-3-acetate esterase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Reference proteome.
FT CHAIN 1..300
FT /note="Uncharacterized carboxylesterase nap"
FT /id="PRO_0000360816"
FT ACT_SITE 274
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT SITE 130
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
FT HELIX 13..26
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 34..38
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 43..51
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 57..61
FT /evidence="ECO:0007829|PDB:2R11"
FT TURN 64..66
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 68..71
FT /evidence="ECO:0007829|PDB:2R11"
FT TURN 72..74
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 75..81
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 82..87
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 92..95
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 105..118
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 122..129
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 131..142
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 147..154
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 156..158
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 164..171
FT /evidence="ECO:0007829|PDB:2R11"
FT TURN 172..174
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 178..186
FT /evidence="ECO:0007829|PDB:2R11"
FT TURN 187..189
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 195..206
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 209..211
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 220..222
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 227..231
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 237..242
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 250..260
FT /evidence="ECO:0007829|PDB:2R11"
FT STRAND 265..269
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 276..279
FT /evidence="ECO:0007829|PDB:2R11"
FT HELIX 281..292
FT /evidence="ECO:0007829|PDB:2R11"
SQ SEQUENCE 300 AA; 33953 MW; FA818689172007AC CRC64;
MSNHSSSIPE LSDNGIRYYQ TYNESLSLWP VRCKSFYIST RFGQTHVIAS GPEDAPPLVL
LHGALFSSTM WYPNIADWSS KYRTYAVDII GDKNKSIPEN VSGTRTDYAN WLLDVFDNLG
IEKSHMIGLS LGGLHTMNFL LRMPERVKSA AILSPAETFL PFHHDFYKYA LGLTASNGVE
TFLNWMMNDQ NVLHPIFVKQ FKAGVMWQDG SRNPNPNADG FPYVFTDEEL RSARVPILLL
LGEHEVIYDP HSALHRASSF VPDIEAEVIK NAGHVLSMEQ PTYVNERVMR FFNAETGISR
