ID   NAR1_CRYNJ              Reviewed;         650 AA.
AC   P0CP10; Q55MV8; Q5KB85;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Cytosolic Fe-S cluster assembly factor NAR1;
DE   AltName: Full=Nuclear architecture-related protein 1;
GN   Name=NAR1; OrderedLocusNames=CNI03410;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Component of the cytosolic Fe/S protein assembly machinery.
CC       Required for maturation of extramitochondrial Fe/S proteins. May play a
CC       role in the transfer of pre-assembled Fe/S clusters to target
CC       apoproteins (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NARF family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE017349; AAW45585.1; -; Genomic_DNA.
DR   RefSeq; XP_572892.1; XM_572892.1.
DR   AlphaFoldDB; P0CP10; -.
DR   STRING; 5207.AAW45585; -.
DR   PaxDb; P0CP10; -.
DR   EnsemblFungi; AAW45585; AAW45585; CNI03410.
DR   GeneID; 3259452; -.
DR   KEGG; cne:CNI03410; -.
DR   VEuPathDB; FungiDB:CNI03410; -.
DR   eggNOG; KOG2439; Eukaryota.
DR   HOGENOM; CLU_018240_0_2_1; -.
DR   InParanoid; P0CP10; -.
DR   OMA; PHEQRAW; -.
DR   OrthoDB; 705416at2759; -.
DR   Proteomes; UP000002149; Chromosome 9.
DR   GO; GO:0097361; C:CIA complex; IBA:GO_Central.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; ISS:UniProtKB.
DR   InterPro; IPR009016; Fe_hydrogenase.
DR   InterPro; IPR004108; Fe_hydrogenase_lsu_C.
DR   Pfam; PF02906; Fe_hyd_lg_C; 1.
DR   SUPFAM; SSF53920; SSF53920; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome.
FT   CHAIN           1..650
FT                   /note="Cytosolic Fe-S cluster assembly factor NAR1"
FT                   /id="PRO_0000383726"
FT   BINDING         22
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         81
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         84
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         87
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         215
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
FT   BINDING         270
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
FT   BINDING         480
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
FT   BINDING         484
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   650 AA;  70460 MW;  0183EBD964B92F1D CRC64;
     MAFSGALTIT DLDDFLTPSQ ACIIPVRNNK KPAEDEGPTE IHIDSNNNYY EVSTYPSVGH
     DDDIGNSKKA LEKAEINLND CLACSGCITS TESLLITMQS QNEILEFIKT NPTVVDPESP
     CHKPRLPILS ISPQTLASLS AAYATASSRP PIPLLVLLRR IRAFLSQPEK GSWRVWDTTF
     ARHMSLRESV VEFHERKDKK EKGKAAEMPM LASACPGWVC YAEKAQGDML PLLSAARSSQ
     GIIGALAKSW YGHKLQHKPD EIYHVTAMPC YDKKLEASRS DFYSSLYSTR DVDCVLTTGE
     LDLLLQELGF DPHVPIANES TPSYSATEDS PFPELLTHEG SSSGSYLQTI IHDVQRSHPN
     PTRIITREIR GSTDNIEYLI QDTITGQIVF KGAKVYGFRN LQNLVRKVAK ETGIGRSGRG
     AGAGKLSAAV AARRRKAKTA APAATSATTS AEGTDVESIA SLSLVSGEDK KLDFVEVMAC
     PGGCVNGGGQ MKPTVPTPSA PEAMEVDEEG YQRPLPDDGV AVAVNGGSNN VGTGTVAGME
     EGMRWSTKEW VAKVEDIYWT GLPTPPASPP LTASNVNGFA PQVKTNGTTN GQVNNGVDRN
     LQSDQLAEEI IHEVCGDDAS KRWDFMRTRF RKVESDVLSS GGVTHEAVKW