ID   NAR1_PICST              Reviewed;         545 AA.
AC   A3LYR2;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Cytosolic Fe-S cluster assembly factor NAR1;
DE   AltName: Full=Nuclear architecture-related protein 1;
GN   Name=NAR1; ORFNames=PICST_33268;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Component of the cytosolic Fe/S protein assembly machinery.
CC       Required for maturation of extramitochondrial Fe/S proteins. May play a
CC       role in the transfer of pre-assembled Fe/S clusters to target
CC       apoproteins (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NARF family. {ECO:0000305}.
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DR   EMBL; CP000501; ABN68216.2; -; Genomic_DNA.
DR   RefSeq; XP_001386245.2; XM_001386208.1.
DR   AlphaFoldDB; A3LYR2; -.
DR   SMR; A3LYR2; -.
DR   STRING; 4924.XP_001386245.2; -.
DR   EnsemblFungi; ABN68216; ABN68216; PICST_33268.
DR   GeneID; 4840427; -.
DR   KEGG; pic:PICST_33268; -.
DR   eggNOG; KOG2439; Eukaryota.
DR   HOGENOM; CLU_018240_0_1_1; -.
DR   InParanoid; A3LYR2; -.
DR   OMA; PHEQRAW; -.
DR   OrthoDB; 705416at2759; -.
DR   Proteomes; UP000002258; Chromosome 7.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; ISS:UniProtKB.
DR   InterPro; IPR009016; Fe_hydrogenase.
DR   InterPro; IPR004108; Fe_hydrogenase_lsu_C.
DR   Pfam; PF02906; Fe_hyd_lg_C; 1.
DR   SUPFAM; SSF53920; SSF53920; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome.
FT   CHAIN           1..545
FT                   /note="Cytosolic Fe-S cluster assembly factor NAR1"
FT                   /id="PRO_0000383737"
FT   BINDING         20
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         74
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         77
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         80
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000255"
FT   BINDING         188
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
FT   BINDING         243
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
FT   BINDING         454
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
FT   BINDING         458
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   545 AA;  59943 MW;  35EF2CF49971E718 CRC64;
     MSAILSADDL NDFISPGVAC IKPPAQNSDQ KFNSLNENGE VEIQIDSEGN PLEISKIDGK
     QTNLSPAQIS LADCLACSGC ITSAEEVLVA QHSHEELIKA LNEKVDNNST KVFVASISHQ
     SRASLATAYN LSIEEIDKLL INLFINQMGF KYIVGTSIGR KLSLINEAQN LIEKKESEFD
     GPVLSSICPG WVLYAEKTHP YVLPRMSTVK SPQQITGCLL KTLAAHELGV TRNDIYHLSI
     MPCFDKKLES ARPEKYGEQN TSNDVDCVLT AKELVTLLEQ HSDKFQLIPP QAHTITNSAI
     PVVDLYSKCA PRTWPLVQYS WSNDSGSASG GYGYNYLKMY QNHLIMKHPT KYQQEGFSID
     YVKGRNTDLT EMRLMYGSEK LASSAIVNGF RNIQNLVRKL KPTVKPGSTT GKGNALVARR
     RARVAGGITK ASSPAGSDES ADASKCDYVE IMACPNGCIN GGGQINPPED VSEKDWLSAS
     LEKYNSIPLL DLAAMENVDT VAEIMQWSCL FREEFGVSEN RLLKTWFNEV EKPTDSASIL
     LGARW