NAR1_USTMA
ID NAR1_USTMA Reviewed; 827 AA.
AC Q4PAR1; A0A0D1E0T9;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Cytosolic Fe-S cluster assembly factor NAR1;
DE AltName: Full=Nuclear architecture-related protein 1;
GN Name=NAR1; ORFNames=UMAG_02802;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=521 / FGSC 9021;
RA Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the cytosolic Fe/S protein assembly machinery.
CC Required for maturation of extramitochondrial Fe/S proteins. May play a
CC role in the transfer of pre-assembled Fe/S clusters to target
CC apoproteins (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NARF family. {ECO:0000305}.
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DR EMBL; CM003145; KIS69471.1; -; Genomic_DNA.
DR RefSeq; XP_011389150.1; XM_011390848.1.
DR AlphaFoldDB; Q4PAR1; -.
DR STRING; 5270.UM02802P0; -.
DR PRIDE; Q4PAR1; -.
DR EnsemblFungi; KIS69471; KIS69471; UMAG_02802.
DR GeneID; 23563460; -.
DR KEGG; uma:UMAG_02802; -.
DR VEuPathDB; FungiDB:UMAG_02802; -.
DR eggNOG; KOG2439; Eukaryota.
DR HOGENOM; CLU_018240_0_2_1; -.
DR OMA; AVWLDWI; -.
DR OrthoDB; 705416at2759; -.
DR Proteomes; UP000000561; Chromosome 6.
DR GO; GO:0097361; C:CIA complex; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0051536; F:iron-sulfur cluster binding; ISS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; ISS:UniProtKB.
DR InterPro; IPR009016; Fe_hydrogenase.
DR InterPro; IPR004108; Fe_hydrogenase_lsu_C.
DR Pfam; PF02906; Fe_hyd_lg_C; 1.
DR SUPFAM; SSF53920; SSF53920; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome.
FT CHAIN 1..827
FT /note="Cytosolic Fe-S cluster assembly factor NAR1"
FT /id="PRO_0000383740"
FT REGION 57..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 209..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 599..637
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 709..739
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 60..77
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 600..624
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 711..739
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 22
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 92
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 95
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 98
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 246
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT BINDING 307
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT BINDING 591
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT BINDING 595
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 827 AA; 89536 MW; 11DF8FD087637729 CRC64;
MAFSGALTLT DLNDYLGPSQ ACIKPVQAAD VPSQDHSDVS ALAASASTHI AIDHDGAYYE
SSTPPSSSLS AADSRPRQRT KLETAQISLN DCLACSGCVT SAESVLITMQ SQDEMRRAIA
ELNQSNANKL VVASISTQSL ASLSAKYTFQ HPQPSSSRSA STSTLPLRVL LHRISYFLKT
VFGFDHVYDT TFARHIALKE HQREFFQRRE NARKRAKLSN APADDDDRLH PDQVDGPTLP
MLASACPGWI CYAEKTHGEL LPYISTTKSP QQVAGVIAKR FLPERLGLLA PSAPDQPSIY
HVTVMPCYDK KLEASRPDFY DDITGTKEVD CVLTTGELDK LMLDEAFDIC TPVPGEQEAI
QESIAELSLQ QAFNTPNPDV GSASVSMMPR LPRLPQLLDQ PGSSSGGYLF LLMRAVWLDW
ISVHWDSLPL SVREQGILPK LDVRVIRTTD FTEFVLRAPT QLVEPCNDDT SQSSILFRGA
QCYGFRNLQN LVRKLQKQTG VRNTRGAAAR LVDADGNAIG AAAARNRASS AKARARGRGG
MMRRARAGAA AVVKSSPLNP DAEAVDVTQL QLSTAHEEDE RGYDYVEVMA CPSGCVNGGG
QIRPPTQSDV DVTRSSTTVD NHATDPEGYT KGGWAADQAG DHDGLELMLN KTCSNKTSAV
PDEADEEKEV RGWQGTSKEW VRRVEEAYWQ DSSVAQTRVT VAAVNRAVDQ GGANDSGSST
PTLVGSGANT PLSSSNAKSI RVEDHQRLLE SLAVSNAERA QRTRVVKLGG DAMAYADVLA
ELVVNELCLL AAPAGDALAL DSARDKLFRT QYRAVQDEAV NGLAVQW
