NAR21_ORYSJ
ID NAR21_ORYSJ Reviewed; 206 AA.
AC Q6ZI50; A0A0P0VL90;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=High-affinity nitrate transporter-activating protein 2.1;
DE Short=OsNAR2.1;
DE Flags: Precursor;
GN Name=NAR2.1; OrderedLocusNames=Os02g0595900, LOC_Os02g38230;
GN ORFNames=OJ1126_D09.33, OsJ_07366;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=21220781; DOI=10.1093/jxb/erq403;
RA Feng H., Yan M., Fan X., Li B., Shen Q., Miller A.J., Xu G.;
RT "Spatial expression and regulation of rice high-affinity nitrate
RT transporters by nitrogen and carbon status.";
RL J. Exp. Bot. 62:2319-2332(2011).
RN [6]
RP INDUCTION BY NITRATE.
RX PubMed=20862512; DOI=10.1007/s10265-010-0375-9;
RA Takayanagi S., Takagi Y., Araki R., Hasegawa H.;
RT "High-affinity nitrate uptake by rice (Oryza sativa) coleoptiles.";
RL J. Plant Res. 124:305-309(2011).
RN [7]
RP FUNCTION, INTERACTION WITH NRT2.1; NRT2.2 AND NRT2.3, AND INDUCTION.
RX PubMed=21486304; DOI=10.1111/j.1365-3040.2011.02335.x;
RA Yan M., Fan X., Feng H., Miller A.J., Shen Q., Xu G.;
RT "Rice OsNAR2.1 interacts with OsNRT2.1, OsNRT2.2 and OsNRT2.3a nitrate
RT transporters to provide uptake over high and low concentration ranges.";
RL Plant Cell Environ. 34:1360-1372(2011).
CC -!- FUNCTION: Acts as a dual component transporter with NTR2.1, NRT2.2 and
CC NRT2.3. Required for high-affinity nitrate transport. Involved in the
CC regulation of NRT2.1, NRT2.2 and NRT2.3 expression, and in both, HATS
CC (high-affinity transport system) and LATS (low-affinity transport
CC system) activities in plant roots. Imports nitrate with high affinity
CC when expressed with NTR2.1, NTR2.2 or NTR2.3 in a heterologous system
CC (Xenopus oocytes). {ECO:0000269|PubMed:21220781,
CC ECO:0000269|PubMed:21486304}.
CC -!- SUBUNIT: Heterotetramer composed of two NRT2.1, NRT2.2 or NRT2.3 and
CC two NAR2.1 (By similarity). Interacts with NRT2.1, NRT2.2 and isoform 1
CC of NRT2.3. {ECO:0000250, ECO:0000269|PubMed:21486304}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in epidermal cells of primary and lateral
CC roots, root-shoot junction zone, vascular tissues of adventitious root
CC primordia, stems and coleoptiles of germinating seeds.
CC {ECO:0000269|PubMed:21220781}.
CC -!- INDUCTION: Circadian-regulation with a peak in the middle of the
CC morning. Induced by nitrate and sucrose in roots. Down-regulated by
CC asparagine and aspartate in roots. {ECO:0000269|PubMed:20862512,
CC ECO:0000269|PubMed:21220781, ECO:0000269|PubMed:21486304}.
CC -!- MISCELLANEOUS: Plants silencing NAR2.1 have decreased nitrate uptake in
CC roots under both high and low nitrate conditions.
CC {ECO:0000305|PubMed:21220781}.
CC -!- SIMILARITY: Belongs to the NAR2 family. {ECO:0000305}.
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DR EMBL; AP004023; BAD16835.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09231.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS79551.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ23664.1; -; Genomic_DNA.
DR RefSeq; XP_015623791.1; XM_015768305.1.
DR AlphaFoldDB; Q6ZI50; -.
DR STRING; 4530.OS02T0595900-00; -.
DR PaxDb; Q6ZI50; -.
DR PRIDE; Q6ZI50; -.
DR EnsemblPlants; Os02t0595900-01; Os02t0595900-01; Os02g0595900.
DR GeneID; 4329861; -.
DR Gramene; Os02t0595900-01; Os02t0595900-01; Os02g0595900.
DR KEGG; osa:4329861; -.
DR eggNOG; ENOG502RXTZ; Eukaryota.
DR HOGENOM; CLU_093989_0_0_1; -.
DR InParanoid; Q6ZI50; -.
DR OrthoDB; 1249242at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6ZI50; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:UniProtKB.
DR GO; GO:0010167; P:response to nitrate; IBA:GO_Central.
DR InterPro; IPR016605; Transptr_NO3_Nar2.
DR PANTHER; PTHR34806; PTHR34806; 1.
DR Pfam; PF16974; NAR2; 1.
DR PIRSF; PIRSF012939; Transpt_NO3_Nar2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Nitrate assimilation; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..206
FT /note="High-affinity nitrate transporter-activating protein
FT 2.1"
FT /id="PRO_0000430008"
FT TRANSMEM 180..200
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 206 AA; 21518 MW; 46D1ACC9DEB03CEF CRC64;
MARLAGVAAL SLVLVLLGAG VPRPAAAAAA KTQVFLSKLP KALVVGVSPK HGEVVHAGEN
TVTVTWSLNT SEPAGADAAF KSVKVKLCYA PASRTDRGWR KASDDLHKDK ACQFKVTVQP
YAAGAGRFDY VVARDIPTAS YFVRAYAVDA SGTEVAYGQS SPDAAFDVAG ITGIHASLKV
AAGVFSTFSI AALAFFFVVE KRKKDK
