ID   NAR22_ORYSJ             Reviewed;         210 AA.
AC   Q7XK12; A0A0N7KJ87;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Probable high-affinity nitrate transporter-activating protein 2.2;
DE            Short=OsNAR2.2;
DE   Flags: Precursor;
GN   Name=NAR2.2; OrderedLocusNames=Os04g0480200, LOC_Os04g40410;
GN   ORFNames=OSJNBa0044K18.32;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [6]
RP   LACK OF INDUCTION BY NITRATE.
RX   PubMed=21220781; DOI=10.1093/jxb/erq403;
RA   Feng H., Yan M., Fan X., Li B., Shen Q., Miller A.J., Xu G.;
RT   "Spatial expression and regulation of rice high-affinity nitrate
RT   transporters by nitrogen and carbon status.";
RL   J. Exp. Bot. 62:2319-2332(2011).
CC   -!- FUNCTION: Involved in nitrate transport. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Not induced by nitrate in roots.
CC   -!- SIMILARITY: Belongs to the NAR2 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL606595; CAE05891.1; -; Genomic_DNA.
DR   EMBL; AP008210; BAF15013.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS89729.1; -; Genomic_DNA.
DR   EMBL; AK109571; BAG98803.1; -; mRNA.
DR   RefSeq; XP_015634457.1; XM_015778971.1.
DR   AlphaFoldDB; Q7XK12; -.
DR   STRING; 4530.OS04T0480200-01; -.
DR   PaxDb; Q7XK12; -.
DR   PRIDE; Q7XK12; -.
DR   EnsemblPlants; Os04t0480200-01; Os04t0480200-01; Os04g0480200.
DR   GeneID; 4336175; -.
DR   Gramene; Os04t0480200-01; Os04t0480200-01; Os04g0480200.
DR   KEGG; osa:4336175; -.
DR   eggNOG; ENOG502RXTZ; Eukaryota.
DR   HOGENOM; CLU_093989_0_0_1; -.
DR   InParanoid; Q7XK12; -.
DR   OMA; ANDHEVA; -.
DR   OrthoDB; 1249242at2759; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q7XK12; OS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0010167; P:response to nitrate; IBA:GO_Central.
DR   InterPro; IPR016605; Transptr_NO3_Nar2.
DR   PANTHER; PTHR34806; PTHR34806; 1.
DR   Pfam; PF16974; NAR2; 1.
DR   PIRSF; PIRSF012939; Transpt_NO3_Nar2; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Nitrate assimilation; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..210
FT                   /note="Probable high-affinity nitrate transporter-
FT                   activating protein 2.2"
FT                   /id="PRO_0000430009"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   210 AA;  21536 MW;  685284D1821B6397 CRC64;
     MARFGAVIHR VFLPLLLLLV VLGACHVTPA AAAAGARLSA LAKALVVEAS PRAGQVLHAG
     EDAITVTWSL NATAAAAAAG ADAGYKAVKV TLCYAPASQV GRGWRKAHDD LSKDKACQFK
     IAQQPYDGAG KFEYTVARDV PTASYYVRAY ALDASGARVA YGETAPSASF AVAGITGVTA
     SIEVAAGVLS AFSVAALAVF LVLENKKKNK