NARF_DROME
ID NARF_DROME Reviewed; 477 AA.
AC Q8SYS7; Q5LJW9; Q5LJX0; Q7PLS3;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Probable cytosolic Fe-S cluster assembly factor CG17683;
GN ORFNames=CG17683;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Component of the cytosolic iron-sulfur (Fe/S) protein
CC assembly machinery. Required for maturation of extramitochondrial Fe/S
CC proteins (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=A;
CC IsoId=Q8SYS7-1; Sequence=Displayed;
CC Name=C;
CC IsoId=Q8SYS7-2; Sequence=VSP_038042;
CC Name=E;
CC IsoId=Q8SYS7-3; Sequence=VSP_038040;
CC Name=D;
CC IsoId=Q8SYS7-4; Sequence=VSP_038041, VSP_038043;
CC -!- SIMILARITY: Belongs to the NARF family. {ECO:0000305}.
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DR EMBL; AE013599; EAA46161.1; -; Genomic_DNA.
DR EMBL; AE013599; EAL24588.1; -; Genomic_DNA.
DR EMBL; AE013599; EAL24589.1; -; Genomic_DNA.
DR EMBL; AE013599; EAL24590.1; -; Genomic_DNA.
DR EMBL; AY071338; AAL48960.1; -; mRNA.
DR RefSeq; NP_001036427.1; NM_001042962.2. [Q8SYS7-3]
DR RefSeq; NP_001036428.1; NM_001042963.2. [Q8SYS7-1]
DR RefSeq; NP_001036429.1; NM_001042964.2. [Q8SYS7-4]
DR RefSeq; NP_001036430.1; NM_001042965.2. [Q8SYS7-2]
DR AlphaFoldDB; Q8SYS7; -.
DR SMR; Q8SYS7; -.
DR IntAct; Q8SYS7; 1.
DR STRING; 7227.FBpp0110523; -.
DR PaxDb; Q8SYS7; -.
DR DNASU; 3355011; -.
DR EnsemblMetazoa; FBtr0111298; FBpp0110523; FBgn0262115. [Q8SYS7-1]
DR EnsemblMetazoa; FBtr0111299; FBpp0110563; FBgn0262115. [Q8SYS7-2]
DR EnsemblMetazoa; FBtr0111300; FBpp0110581; FBgn0262115. [Q8SYS7-4]
DR EnsemblMetazoa; FBtr0111301; FBpp0110483; FBgn0262115. [Q8SYS7-3]
DR GeneID; 3355011; -.
DR KEGG; dme:Dmel_CG17683; -.
DR UCSC; CG17683-RA; d. melanogaster. [Q8SYS7-1]
DR UCSC; CG17683-RB; d. melanogaster.
DR UCSC; CG17683-RC; d. melanogaster.
DR UCSC; CG17683-RE; d. melanogaster.
DR FlyBase; FBgn0262115; CG17683.
DR VEuPathDB; VectorBase:FBgn0262115; -.
DR eggNOG; KOG2439; Eukaryota.
DR GeneTree; ENSGT00940000153514; -.
DR InParanoid; Q8SYS7; -.
DR OMA; PHEQRAW; -.
DR PhylomeDB; Q8SYS7; -.
DR BioGRID-ORCS; 3355011; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 3355011; -.
DR PRO; PR:Q8SYS7; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0262115; Expressed in cleaving embryo and 22 other tissues.
DR ExpressionAtlas; Q8SYS7; baseline and differential.
DR Genevisible; Q8SYS7; DM.
DR GO; GO:0097361; C:CIA complex; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; ISS:UniProtKB.
DR InterPro; IPR009016; Fe_hydrogenase.
DR InterPro; IPR004108; Fe_hydrogenase_lsu_C.
DR InterPro; IPR003149; Fe_hydrogenase_ssu.
DR Pfam; PF02906; Fe_hyd_lg_C; 1.
DR Pfam; PF02256; Fe_hyd_SSU; 1.
DR SMART; SM00902; Fe_hyd_SSU; 1.
DR SUPFAM; SSF53920; SSF53920; 1.
PE 2: Evidence at transcript level;
KW 4Fe-4S; Alternative splicing; Iron; Iron-sulfur; Metal-binding;
KW Reference proteome.
FT CHAIN 1..477
FT /note="Probable cytosolic Fe-S cluster assembly factor
FT CG17683"
FT /id="PRO_0000383702"
FT BINDING 23
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 68
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 71
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 74
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255"
FT BINDING 187
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT BINDING 243
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT BINDING 395
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT BINDING 399
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..181
FT /note="Missing (in isoform E)"
FT /evidence="ECO:0000305"
FT /id="VSP_038040"
FT VAR_SEQ 1..47
FT /note="Missing (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_038041"
FT VAR_SEQ 22..25
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000305"
FT /id="VSP_038042"
FT VAR_SEQ 48..52
FT /note="FEESE -> MVSLQ (in isoform D)"
FT /evidence="ECO:0000305"
FT /id="VSP_038043"
SQ SEQUENCE 477 AA; 54534 MW; 4684F53AC42A4C28 CRC64;
MSRLSRALQL TDIDDFITPS QICIKPVQID KARSKTGAKI KIKGDGCFEE SESGNLKLNK
VDISLQDCLA CSGCITSAEE VLITQQSREE LLKVLQENSK NKASEDWDNV RTIVFTLATQ
PILSLAYRYQ IGVEDAARHL NGYFRSLGAD YVLSTKVADD IALLECRQEF VDRYRENENL
TMLSSSCPGW VCYAEKTHGN FLLPYVSTTR SPQQIMGVLV KQILADKMNV PASRIYHVTV
MPCYDKKLEA SREDFFSKAN NSRDVDCVIT SVEVEQLLSE AQQPLSQYDL LDLDWPWSNV
RPEFMVWAHE KTLSGGYAEH IFKYAAKHIF NEDLKTELEF KQLKNRDFRE IILKQNGKTV
LKFAIANGFR NIQNLVQKLK REKVSNYHFV EVMACPSGCI NGGAQIRPTT GQHVRELTRK
LEELYQNLPR SEPENSLTKH IYNDFLDGFQ SDKSYDVLHT RYHDVVSELS ISLNINW
