NARF_HUMAN
ID NARF_HUMAN Reviewed; 456 AA.
AC Q9UHQ1; A6NCJ3; B3KPX2; K4DI98; Q96AY9; Q9BWC6;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Nuclear prelamin A recognition factor;
DE AltName: Full=Iron-only hydrogenase-like protein 2;
DE Short=IOP2;
GN Name=NARF;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND INTERACTION WITH LMNA.
RX PubMed=10514485; DOI=10.1074/jbc.274.42.30008;
RA Barton R.M., Worman H.J.;
RT "Prenylated prelamin A interacts with Narf, a novel nuclear protein.";
RL J. Biol. Chem. 274:30008-30018(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4).
RC TISSUE=Embryo, and Skeletal muscle;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16625196; DOI=10.1038/nature04689;
RA Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R.,
RA Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A.,
RA Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J.,
RA Chang J.L., Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J.,
RA DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S.,
RA Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E.,
RA Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K.,
RA LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J.,
RA Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A.,
RA Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K.,
RA Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D.,
RA Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A.,
RA Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.;
RT "DNA sequence of human chromosome 17 and analysis of rearrangement in the
RT human lineage.";
RL Nature 440:1045-1049(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Lung, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-29, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- SUBUNIT: Interacts with LMNA and binds to the farnesylated C-terminal
CC domain. {ECO:0000269|PubMed:10514485}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10514485}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q9UHQ1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9UHQ1-2; Sequence=VSP_025690;
CC Name=3;
CC IsoId=Q9UHQ1-3; Sequence=VSP_025691;
CC Name=4;
CC IsoId=Q9UHQ1-4; Sequence=VSP_046371;
CC -!- TISSUE SPECIFICITY: Ubiquitous. Predominantly expressed in skeletal
CC muscle, heart and brain. {ECO:0000269|PubMed:10514485}.
CC -!- SIMILARITY: Belongs to the NARF family. {ECO:0000305}.
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DR EMBL; AF128406; AAD51446.1; -; mRNA.
DR EMBL; AK000929; BAA91432.1; -; mRNA.
DR EMBL; AK056966; BAG51834.1; -; mRNA.
DR EMBL; AC124287; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC132938; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471099; EAW89794.1; -; Genomic_DNA.
DR EMBL; CH471099; EAW89798.1; -; Genomic_DNA.
DR EMBL; BC000438; AAH00438.1; -; mRNA.
DR EMBL; BC016440; AAH16440.1; -; mRNA.
DR CCDS; CCDS32777.1; -. [Q9UHQ1-1]
DR CCDS; CCDS42403.1; -. [Q9UHQ1-3]
DR CCDS; CCDS42404.1; -. [Q9UHQ1-4]
DR RefSeq; NP_001033707.1; NM_001038618.2. [Q9UHQ1-4]
DR RefSeq; NP_001077077.1; NM_001083608.1. [Q9UHQ1-3]
DR RefSeq; NP_036468.1; NM_012336.3. [Q9UHQ1-1]
DR RefSeq; NP_114174.1; NM_031968.2. [Q9UHQ1-2]
DR RefSeq; XP_005256397.1; XM_005256340.3. [Q9UHQ1-4]
DR RefSeq; XP_011521843.1; XM_011523541.2. [Q9UHQ1-4]
DR AlphaFoldDB; Q9UHQ1; -.
DR SMR; Q9UHQ1; -.
DR BioGRID; 117709; 27.
DR IntAct; Q9UHQ1; 17.
DR MINT; Q9UHQ1; -.
DR STRING; 9606.ENSP00000309899; -.
DR iPTMnet; Q9UHQ1; -.
DR PhosphoSitePlus; Q9UHQ1; -.
DR BioMuta; NARF; -.
DR DMDM; 74735021; -.
DR EPD; Q9UHQ1; -.
DR jPOST; Q9UHQ1; -.
DR MassIVE; Q9UHQ1; -.
DR MaxQB; Q9UHQ1; -.
DR PaxDb; Q9UHQ1; -.
DR PeptideAtlas; Q9UHQ1; -.
DR PRIDE; Q9UHQ1; -.
DR ProteomicsDB; 84396; -. [Q9UHQ1-1]
DR ProteomicsDB; 84397; -. [Q9UHQ1-2]
DR ProteomicsDB; 84398; -. [Q9UHQ1-3]
DR Antibodypedia; 33014; 195 antibodies from 27 providers.
DR DNASU; 26502; -.
DR Ensembl; ENST00000309794.16; ENSP00000309899.10; ENSG00000141562.19. [Q9UHQ1-1]
DR Ensembl; ENST00000345415.11; ENSP00000283996.9; ENSG00000141562.19. [Q9UHQ1-3]
DR Ensembl; ENST00000390006.8; ENSP00000374656.4; ENSG00000141562.19. [Q9UHQ1-4]
DR GeneID; 26502; -.
DR KEGG; hsa:26502; -.
DR MANE-Select; ENST00000309794.16; ENSP00000309899.10; NM_012336.4; NP_036468.1.
DR UCSC; uc002kff.5; human. [Q9UHQ1-1]
DR CTD; 26502; -.
DR GeneCards; NARF; -.
DR HGNC; HGNC:29916; NARF.
DR HPA; ENSG00000141562; Low tissue specificity.
DR MIM; 605349; gene.
DR neXtProt; NX_Q9UHQ1; -.
DR OpenTargets; ENSG00000141562; -.
DR PharmGKB; PA142671294; -.
DR VEuPathDB; HostDB:ENSG00000141562; -.
DR eggNOG; KOG2439; Eukaryota.
DR GeneTree; ENSGT00940000153514; -.
DR HOGENOM; CLU_018240_0_0_1; -.
DR InParanoid; Q9UHQ1; -.
DR OrthoDB; 705416at2759; -.
DR PhylomeDB; Q9UHQ1; -.
DR TreeFam; TF106273; -.
DR PathwayCommons; Q9UHQ1; -.
DR SignaLink; Q9UHQ1; -.
DR BioGRID-ORCS; 26502; 14 hits in 1078 CRISPR screens.
DR ChiTaRS; NARF; human.
DR GeneWiki; NARF; -.
DR GenomeRNAi; 26502; -.
DR Pharos; Q9UHQ1; Tbio.
DR PRO; PR:Q9UHQ1; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q9UHQ1; protein.
DR Bgee; ENSG00000141562; Expressed in left testis and 196 other tissues.
DR ExpressionAtlas; Q9UHQ1; baseline and differential.
DR Genevisible; Q9UHQ1; HS.
DR GO; GO:0005638; C:lamin filament; IDA:HGNC-UCL.
DR GO; GO:0005652; C:nuclear lamina; TAS:ProtInc.
DR GO; GO:0031981; C:nuclear lumen; IDA:HGNC-UCL.
DR GO; GO:0005730; C:nucleolus; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005521; F:lamin binding; IPI:HGNC-UCL.
DR InterPro; IPR009016; Fe_hydrogenase.
DR InterPro; IPR004108; Fe_hydrogenase_lsu_C.
DR InterPro; IPR003149; Fe_hydrogenase_ssu.
DR Pfam; PF02906; Fe_hyd_lg_C; 1.
DR Pfam; PF02256; Fe_hyd_SSU; 1.
DR SMART; SM00902; Fe_hyd_SSU; 1.
DR SUPFAM; SSF53920; SSF53920; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..456
FT /note="Nuclear prelamin A recognition factor"
FT /id="PRO_0000288479"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..21
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 29
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..59
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_046371"
FT VAR_SEQ 38..85
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025691"
FT VAR_SEQ 256
FT /note="S -> SEISQAWWCTPVITATREAAARESLEPGRQRLQRDKIAPLDSSLGGG
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025690"
FT CONFLICT 154
FT /note="R -> L (in Ref. 2; BAG51834)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 456 AA; 51156 MW; F615D812E7FC7D0F CRC64;
MKCEHCTRKE CSKKTKTDDQ ENVSADAPSP AQENGEKGEF HKLADAKIFL SDCLACDSCM
TAEEGVQLSQ QNAKDFFRVL NLNKKCDTSK HKVLVVSVCP QSLPYFAAKF NLSVTDASRR
LCGFLKSLGV HYVFDTTIAA DFSILESQKE FVRRYRQHSE EERTLPMLTS ACPGWVRYAE
RVLGRPITAH LCTAKSPQQV MGSLVKDYFA RQQNLSPEKI FHVIVAPCYD KKLEALQESL
PPALHGSRGA DCVLTSGEIA QIMEQGDLSV RDAAVDTLFG DLKEDKVTRH DGASSDGHLA
HIFRHAAKEL FNEDVEEVTY RALRNKDFQE VTLEKNGEVV LRFAAAYGFR NIQNMILKLK
KGKFPFHFVE VLACAGGCLN GRGQAQTPDG HADKALLRQM EGIYADIPVR RPESSAHVQE
LYQEWLEGIN SPKAREVLHT TYQSQERGTH SLDIKW
