ID   NARJ_ECO57              Reviewed;         236 AA.
AC   P0AF27; P11351;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Nitrate reductase molybdenum cofactor assembly chaperone NarJ;
DE   AltName: Full=Redox enzyme maturation protein NarJ;
GN   Name=narJ; OrderedLocusNames=Z2003, ECs1731;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Chaperone required for proper molybdenum cofactor insertion
CC       and final assembly of the membrane-bound respiratory nitrate reductase
CC       1. {ECO:0000250}.
CC   -!- SUBUNIT: Binds specifically to the NarG subunit of the apoenzyme
CC       complex at two distinct sites, one interfering with membrane anchoring
CC       and another being involved in molybdenum insertion. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NarJ/NarW family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE005174; AAG56086.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB35154.1; -; Genomic_DNA.
DR   PIR; C90845; C90845.
DR   RefSeq; NP_309758.1; NC_002695.1.
DR   RefSeq; WP_000571681.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AF27; -.
DR   SMR; P0AF27; -.
DR   STRING; 155864.EDL933_1933; -.
DR   EnsemblBacteria; AAG56086; AAG56086; Z2003.
DR   EnsemblBacteria; BAB35154; BAB35154; ECs_1731.
DR   GeneID; 913130; -.
DR   KEGG; ece:Z2003; -.
DR   KEGG; ecs:ECs_1731; -.
DR   PATRIC; fig|386585.9.peg.1832; -.
DR   eggNOG; COG2180; Bacteria.
DR   HOGENOM; CLU_084469_0_0_6; -.
DR   OMA; CELFDRG; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   GO; GO:0051131; P:chaperone-mediated protein complex assembly; ISS:UniProtKB.
DR   GO; GO:0042128; P:nitrate assimilation; ISS:UniProtKB.
DR   InterPro; IPR020945; DMSO/NO3_reduct_chaperone.
DR   InterPro; IPR003765; NO3_reductase_chaperone_NarJ.
DR   InterPro; IPR036411; TorD-like_sf.
DR   PANTHER; PTHR43680; PTHR43680; 1.
DR   Pfam; PF02613; Nitrate_red_del; 1.
DR   SUPFAM; SSF89155; SSF89155; 1.
DR   TIGRFAMs; TIGR00684; narJ; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nitrate assimilation; Reference proteome.
FT   CHAIN           1..236
FT                   /note="Nitrate reductase molybdenum cofactor assembly
FT                   chaperone NarJ"
FT                   /id="PRO_0000096726"
SQ   SEQUENCE   236 AA;  26449 MW;  17306C34A57DF1E2 CRC64;
     MIELVIVSRL LEYPDAALWQ HQQEMFEAIA ASKNLPKEDA HALGIFLRDL TTMDPLDAQA
     QYSELFDRGR ATSLLLFEHV HGESRDRGQA MVDLLAQYEQ HGLQLNSREL PDHLPLYLEY
     LAQLPQSEAV EGLKDIAPIL ALLSARLQQR ESRYAVLFDL LLKLANTAID SDKVAEKIAD
     EARDDTPQAL DAVWEEEQVK FFADKGCGDS AITAHQRRFA GAVAPQYLNI TTGGQH