ID   NARK1_THETH             Reviewed;         443 AA.
AC   Q9RA46;
DT   10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 2.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Probable nitrate/nitrite antiporter NarK1 {ECO:0000305};
GN   Name=narK1 {ECO:0000303|PubMed:10735860};
GN   Synonyms=narK {ECO:0000312|EMBL:CAB65479.2};
OS   Thermus thermophilus.
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NAR1;
RX   PubMed=9540837; DOI=10.1016/s0167-4781(97)00183-8;
RA   Ramirez-Arcos S., Fernandez-Herrero L.A., Berenguer J.;
RT   "A thermophilic nitrate reductase is responsible for the strain specific
RT   anaerobic growth of Thermus thermophilus HB8.";
RL   Biochim. Biophys. Acta 1396:215-227(1998).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=NAR1;
RX   PubMed=10735860; DOI=10.1128/jb.182.8.2179-2183.2000;
RA   Ramirez-Arcos S., Moreno R., Zafra O., Castan P., Valles C., Berenguer J.;
RT   "Two nitrate/nitrite transporters are encoded within the mobilizable
RT   plasmid for nitrate respiration of Thermus thermophilus HB8.";
RL   J. Bacteriol. 182:2179-2183(2000).
CC   -!- FUNCTION: Probable nitrate/nitrite antiporter that may be involved in
CC       nitrate import and nitrite export during anaerobic growth.
CC       {ECO:0000269|PubMed:10735860}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=nitrate(in) + nitrite(out) = nitrate(out) + nitrite(in);
CC         Xref=Rhea:RHEA:28743, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632;
CC         Evidence={ECO:0000305|PubMed:10735860};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28745;
CC         Evidence={ECO:0000305|PubMed:10735860};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Mutation has minor effects on the ability to grow
CC       anaerobically. In contrast, narK1/narK2 double mutant is unable to grow
CC       anaerobically. {ECO:0000269|PubMed:10735860}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; Y10124; CAB65479.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q9RA46; -.
DR   SMR; Q9RA46; -.
DR   TCDB; 2.A.1.8.8; the major facilitator superfamily (mfs).
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   CDD; cd17341; MFS_NRT2_like; 1.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR044772; NO3_transporter.
DR   PANTHER; PTHR23515; PTHR23515; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell membrane; Membrane; Nitrate assimilation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..443
FT                   /note="Probable nitrate/nitrite antiporter NarK1"
FT                   /id="PRO_0000439859"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        142..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        230..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        255..275
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        298..318
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        329..349
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        368..388
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        401..421
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   443 AA;  49095 MW;  040632F0AF680BDA CRC64;
     MIHDPAQLEK ERPDRLRVLW LSTLAFTLMF AAWLMFGVLG VPIRKEFGLS DVQLSWISAL
     AILNGSLWRL LAGILADRYG GRLVFTLMLF FTAIPAYLVS RAGSYEELLL YAFLVGFAGN
     SFSVGIAWNS AWFPKDQQGF ALGVFGAGNV GASVTKFIGP ALIASVPASG YLGGLIPGGW
     RFIPFLYAVL LVLMGFVLWF GTPRKDKRPG QGRPFLDMLK PLRYVRVWRF SLYYVVVFGA
     YVALSAWLPK YYVDVFGLPL HEAALLTALF IFPASLLRPV GGYLSDRFGA RRIMYWTFGI
     ILLASGVLMM PEGHIVLYTK QGAKEVMQFT MGVELFTLLV FLIGVGMGIG KAAVYKHIPT
     YFPQDVGAVG GLVGMLGALG GFFLPPLFAY AQAWTGLPQM TFFVLFLLAA ISFLWMHLTV
     LKLLQQEAQH LKNDFELKGD RPC