ID   NARK2_MYCTO             Reviewed;         395 AA.
AC   P9WJY6; L0TAG9; P71995; Q7D820;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Probable nitrate/nitrite transporter NarK2;
GN   Name=narK2; Synonyms=narK-3; OrderedLocusNames=MT1779;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [2]
RP   INDUCTION BY NITRIC OXIDE (NO) AND BY HYPOXIA, AND DORMANCY REGULON.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12953092; DOI=10.1084/jem.20030205;
RA   Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M.,
RA   Sherman D.R., Schoolnik G.K.;
RT   "Inhibition of respiration by nitric oxide induces a Mycobacterium
RT   tuberculosis dormancy program.";
RL   J. Exp. Med. 198:705-713(2003).
CC   -!- FUNCTION: Involved in excretion of nitrite produced by the
CC       dissimilatory reduction of nitrate. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: A member of the dormancy regulon. Induced in response to
CC       reduced oxygen tension (hypoxia) and low levels of nitric oxide (NO).
CC       {ECO:0000269|PubMed:12953092}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK46052.1; -; Genomic_DNA.
DR   PIR; D70688; D70688.
DR   RefSeq; WP_003898992.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WJY6; -.
DR   SMR; P9WJY6; -.
DR   EnsemblBacteria; AAK46052; AAK46052; MT1779.
DR   KEGG; mtc:MT1779; -.
DR   PATRIC; fig|83331.31.peg.1909; -.
DR   HOGENOM; CLU_001265_14_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   CDD; cd17341; MFS_NRT2_like; 1.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR044772; NO3_transporter.
DR   PANTHER; PTHR23515; PTHR23515; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Nitrate assimilation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..395
FT                   /note="Probable nitrate/nitrite transporter NarK2"
FT                   /id="PRO_0000427748"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        157..177
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        301..321
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        333..353
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        365..385
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   395 AA;  41109 MW;  D901788EE33625A5 CRC64;
     MRGQAANLVL ATWISVVNFW AWNLIGPLST SYARDMSLSS AEASLLVATP ILVGALGRIV
     TGPLTDRFGG RAMLIAVTLA SILPVLAVGV AATMGSYALL VFFGLFLGVA GTIFAVGIPF
     ANNWYQPARR GFSTGVFGMG MVGTALSAFF TPRFVRWFGL FTTHAIVAAA LASTAVVAMV
     VLRDAPYFRP NADPVLPRLK AAARLPVTWE MSFLYAIVFG GFVAFSNYLP TYITTIYGFS
     TVDAGARTAG FALAAVLARP VGGWLSDRIA PRHVVLASLA GTALLAFAAA LQPPPEVWSA
     ATFITLAVCL GVGTGGVFAW VARRAPAASV GSVTGIVAAA GGLGGYFPPL VMGATYDPVD
     NDYTVGLLLL VATALVACTY TALHAREPVS EEASR