ID   NARK2_THETH             Reviewed;         443 AA.
AC   Q9RA45;
DT   10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 2.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Probable nitrate/nitrite antiporter NarK2 {ECO:0000305};
GN   Name=narK2 {ECO:0000303|PubMed:10735860};
GN   Synonyms=narT {ECO:0000312|EMBL:CAB65480.2};
OS   Thermus thermophilus.
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NAR1;
RX   PubMed=9540837; DOI=10.1016/s0167-4781(97)00183-8;
RA   Ramirez-Arcos S., Fernandez-Herrero L.A., Berenguer J.;
RT   "A thermophilic nitrate reductase is responsible for the strain specific
RT   anaerobic growth of Thermus thermophilus HB8.";
RL   Biochim. Biophys. Acta 1396:215-227(1998).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=NAR1;
RX   PubMed=10735860; DOI=10.1128/jb.182.8.2179-2183.2000;
RA   Ramirez-Arcos S., Moreno R., Zafra O., Castan P., Valles C., Berenguer J.;
RT   "Two nitrate/nitrite transporters are encoded within the mobilizable
RT   plasmid for nitrate respiration of Thermus thermophilus HB8.";
RL   J. Bacteriol. 182:2179-2183(2000).
CC   -!- FUNCTION: Probable nitrate/nitrite antiporter that may be involved in
CC       nitrate import and nitrite export during anaerobic growth.
CC       {ECO:0000269|PubMed:10735860}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=nitrate(in) + nitrite(out) = nitrate(out) + nitrite(in);
CC         Xref=Rhea:RHEA:28743, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632;
CC         Evidence={ECO:0000305|PubMed:10735860};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28745;
CC         Evidence={ECO:0000305|PubMed:10735860};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Mutation has minor effects on the ability to grow
CC       anaerobically. In contrast, narK1/narK2 double mutant is unable to grow
CC       anaerobically. {ECO:0000269|PubMed:10735860}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR   EMBL; Y10124; CAB65480.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q9RA45; -.
DR   SMR; Q9RA45; -.
DR   TCDB; 2.A.1.8.9; the major facilitator superfamily (mfs).
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0015113; F:nitrite transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   CDD; cd17341; MFS_NRT2_like; 1.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR044772; NO3_transporter.
DR   InterPro; IPR004737; NO3_transporter_NarK/NarU-like.
DR   PANTHER; PTHR23515; PTHR23515; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   TIGRFAMs; TIGR00886; 2A0108; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell membrane; Membrane; Nitrate assimilation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..443
FT                   /note="Probable nitrate/nitrite antiporter NarK2"
FT                   /id="PRO_0000439860"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        256..276
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        292..312
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        314..334
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        346..366
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        383..403
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        409..429
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   443 AA;  49070 MW;  8A9E3AC8C7006E36 CRC64;
     MLKSAKGTWI TDWNPEDPKR WDPALAWRTL WITTFNLTLS FITWYVVSAL VVRLPKVGFE
     LSTTQLFWLT AMPGLAGGTL RIIWTFLPPI LGTRHLVTFS TLLLLIPLLG WGFAVQNTGT
     PYWVLLLLAF LAGIGGGHFS GYMPSTSYFF PKRLQGTALG LQAGIGNFGV SIVQFVTPWI
     IGFALFGSLL GGPQTFTPKP GVSQPIWLQN ATFAWVPFVL LGALLAWVYL RSVPIRANFR
     EQFDIFRDKH TWIMTSLYIM TFGSFSGFSA IFPLLIREVY GKFEGAPDPL RYAFLGPLVG
     SLARVIAGPI SDRLGGAIVT QVSAIGIFLS ALLVTLYTRP TSLDQFPMFV VAMLLIFFFS
     GVGNASTFKQ MPMIFPPRQA GGVIGWTAAV AAYGPFLFST LAAYTQQATG GFTAFFYGLM
     VFYAFNFFLN WYYYARKGAE KPC