NARK_BACSU
ID NARK_BACSU Reviewed; 395 AA.
AC P46907;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Nitrite extrusion protein;
DE AltName: Full=Nitrite facilitator;
GN Name=narK; OrderedLocusNames=BSU37320;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8846791;
RA Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.;
RT "Anaerobic transcription activation in Bacillus subtilis: identification of
RT distinct FNR-dependent and -independent regulatory mechanisms.";
RL EMBO J. 14:5984-5994(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 276.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-171.
RC STRAIN=168;
RX PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA Kunst F., Danchin A., Glaser P.;
RT "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT degrees).";
RL Microbiology 143:3313-3328(1997).
CC -!- FUNCTION: Involved in excretion of nitrite produced by the
CC dissimilatory reduction of nitrate.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; Z49884; CAA90041.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15760.2; -; Genomic_DNA.
DR EMBL; Z97024; CAB09704.1; -; Genomic_DNA.
DR PIR; S60080; S60080.
DR RefSeq; NP_391613.2; NC_000964.3.
DR RefSeq; WP_010886631.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P46907; -.
DR SMR; P46907; -.
DR STRING; 224308.BSU37320; -.
DR PaxDb; P46907; -.
DR EnsemblBacteria; CAB15760; CAB15760; BSU_37320.
DR GeneID; 937055; -.
DR KEGG; bsu:BSU37320; -.
DR PATRIC; fig|224308.179.peg.4043; -.
DR eggNOG; COG2223; Bacteria.
DR InParanoid; P46907; -.
DR OMA; DAWWFMF; -.
DR PhylomeDB; P46907; -.
DR BioCyc; BSUB:BSU37320-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Nitrate assimilation; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..395
FT /note="Nitrite extrusion protein"
FT /id="PRO_0000096727"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 276
FT /note="A -> T (in Ref. 1; CAA90041)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 395 AA; 42926 MW; 040F35C3EC4D9210 CRC64;
MINRQHIQLS LQSLSLVAGF MVWVLISSLI SQITLDIHLS KGEISLVTAI PVILGSLLRI
PLGYLTNRFG ARLMFMVSFI LLLFPVFWIS IADSLFDLIA GGFFLGIGGA VFSIGVTSLP
KYYPKEKHGV VNGIYGAGNI GTAVTTFAAP VIAQAVGWKS TVQMYLILLA VFALLHVLFG
DRHEKKVKVS VKTQIKAVYR NHVLWFLSLF YFITFGAFVA FTIYLPNFLV EHFGLNPADA
GLRTAGFIAV STLLRPAGGF LADKMSPLRI LMFVFAGLTL SGIILSFSPT IGLYTFGSLT
VAVCSGIGNG TVFKLVPFYF SKQAGIANGI VSAMGGLGGF FPPLILASVF QATGQYAIGF
MALSEVALAS FVLVIWMYWQ ERMKTHTERN SQSIN
