ID   NARL_MYCTO              Reviewed;         216 AA.
AC   P9WGM4; L0T7W4; O53856; Q7D965;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Probable transcriptional regulatory protein NarL;
GN   Name=narL; OrderedLocusNames=MT0866;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Member of the two-component regulatory system NarS/NarL that
CC       regulates genes involved in aerobic nitrate metabolism. Upon
CC       phosphorylation by NarS, functions as a transcription regulator by
CC       direct binding to promoter regions of target genes together with DevR
CC       to regulate their expression during aerobic nitrate metabolism.
CC       {ECO:0000250|UniProtKB:P9WGM5}.
CC   -!- SUBUNIT: Monomer in solution. Interacts with DevR.
CC       {ECO:0000250|UniProtKB:P9WGM5}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by NarS. {ECO:0000250|UniProtKB:P9WGM5}.
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DR   EMBL; AE000516; AAK45109.1; -; Genomic_DNA.
DR   PIR; D70813; D70813.
DR   RefSeq; WP_003404388.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WGM4; -.
DR   SMR; P9WGM4; -.
DR   EnsemblBacteria; AAK45109; AAK45109; MT0866.
DR   GeneID; 45424810; -.
DR   KEGG; mtc:MT0866; -.
DR   PATRIC; fig|83331.31.peg.930; -.
DR   HOGENOM; CLU_000445_90_10_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd06170; LuxR_C_like; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR   Pfam; PF00196; GerE; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   PRINTS; PR00038; HTHLUXR.
DR   SMART; SM00421; HTH_LUXR; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS50043; HTH_LUXR_2; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..216
FT                   /note="Probable transcriptional regulatory protein NarL"
FT                   /id="PRO_0000428336"
FT   DOMAIN          10..126
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          148..213
FT                   /note="HTH luxR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   DNA_BIND        172..191
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT   MOD_RES         61
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGM5,
FT                   ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   216 AA;  22916 MW;  4604E6D7C8D132E5 CRC64;
     MSNPQPEKVR VVVGDDHPLF REGVVRALSL SGSVNVVGEA DDGAAALELI KAHLPDVALL
     DYRMPGMDGA QVAAAVRSYE LPTRVLLISA HDEPAIVYQA LQQGAAGFLL KDSTRTEIVK
     AVLDCAKGRD VVAPSLVGGL AGEIRQRAAP VAPVLSARER EVLNRIACGQ SIPAIAAELY
     VAPSTVKTHV QRLYEKLGVS DRAAAVAEAM RQRLLD