NARO_HALVD
ID NARO_HALVD Reviewed; 211 AA.
AC D4GPG1; A0A1U8QYC0; A0A384KE70; L9VGT6;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2016, sequence version 2.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=Transcriptional regulator NarO {ECO:0000303|PubMed:26787768};
DE AltName: Full=Bacterioopsin transcriptional activator {ECO:0000305};
DE AltName: Full=DNA-binding protein NarO {ECO:0000303|PubMed:26787768};
GN Name=narO {ECO:0000303|PubMed:26787768, ECO:0000312|EMBL:ADE01511.2};
GN Synonyms=boa9 {ECO:0000312|EMBL:ADE01511.2};
GN OrderedLocusNames=HVO_B0159 {ECO:0000312|EMBL:ADE01511.2};
GN ORFNames=C498_02845 {ECO:0000312|EMBL:ELY36420.1};
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OG Plasmid pHV3 {ECO:0000312|EMBL:ADE01511.2,
OG ECO:0000312|Proteomes:UP000008243}.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800 {ECO:0000312|EMBL:ADE01511.2};
RN [1] {ECO:0000312|EMBL:ADE01511.2, ECO:0000312|Proteomes:UP000008243}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2 {ECO:0000312|Proteomes:UP000008243};
RC PLASMID=pHV3 {ECO:0000312|EMBL:ADE01511.2};
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
RN [2] {ECO:0000312|EMBL:ELY36420.1, ECO:0000312|Proteomes:UP000011532}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2 {ECO:0000312|Proteomes:UP000011532};
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
RN [3]
RP FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF CYS-17;
RP CYS-81; CYS-83; CYS-91 AND CYS-100.
RC STRAIN=DS2 / DS70 {ECO:0000303|PubMed:26787768};
RX PubMed=26787768; DOI=10.1128/jb.00833-15;
RA Hattori T., Shiba H., Ashiki K., Araki T., Nagashima Y.K., Yoshimatsu K.,
RA Fujiwara T.;
RT "Anaerobic Growth of Haloarchaeon Haloferax volcanii by Denitrification Is
RT Controlled by the Transcription Regulator NarO.";
RL J. Bacteriol. 198:1077-1086(2016).
CC -!- FUNCTION: Activates transcription of the denitrifying genes (nitrate
CC reductase narA and nitrite reductase nirK) under anaerobic conditions.
CC {ECO:0000269|PubMed:26787768}.
CC -!- INDUCTION: Constitutively expressed regardless of cultivation under
CC aerobic or anaerobic conditions. Increased expression under
CC denitrifying conditions. {ECO:0000269|PubMed:26787768}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene are not able to grow
CC anaerobically by denitrification. Inactivated transcription of the
CC denitrifying genes (nitrate reductase narA and nitrite reductase nirK).
CC No effect on growth under aerobic conditions. No effect on dimethyl
CC sulfoxide (DMSO) respiration. {ECO:0000269|PubMed:26787768}.
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DR EMBL; CP001953; ADE01511.2; -; Genomic_DNA.
DR EMBL; AOHU01000022; ELY36420.1; -; Genomic_DNA.
DR RefSeq; WP_004041376.1; NZ_AOHU01000022.1.
DR STRING; 309800.C498_02845; -.
DR EnsemblBacteria; ADE01511; ADE01511; HVO_B0159.
DR EnsemblBacteria; ELY36420; ELY36420; C498_02845.
DR GeneID; 8919217; -.
DR KEGG; hvo:HVO_B0159; -.
DR PATRIC; fig|309800.29.peg.556; -.
DR eggNOG; arCOG02280; Archaea.
DR OrthoDB; 67263at2157; -.
DR Proteomes; UP000008243; Plasmid pHV3.
DR Proteomes; UP000011532; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0071249; P:cellular response to nitrate; IEP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR007050; HTH_bacterioopsin.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04967; HTH_10; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Plasmid; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..211
FT /note="Transcriptional regulator NarO"
FT /id="PRO_0000454762"
FT DOMAIN 154..205
FT /note="HTH bat-type"
FT /evidence="ECO:0000255"
FT MUTAGEN 17
FT /note="C->S: Unable to grow under denitrifying conditions.
FT Normal growth under aerobic conditions."
FT /evidence="ECO:0000269|PubMed:26787768"
FT MUTAGEN 81
FT /note="C->S: Unable to grow under denitrifying conditions.
FT Normal growth under aerobic conditions."
FT /evidence="ECO:0000269|PubMed:26787768"
FT MUTAGEN 83
FT /note="C->S: Unable to grow under denitrifying conditions.
FT Normal growth under aerobic conditions."
FT /evidence="ECO:0000269|PubMed:26787768"
FT MUTAGEN 91
FT /note="C->S: Unable to grow under denitrifying conditions.
FT Normal growth under aerobic conditions."
FT /evidence="ECO:0000269|PubMed:26787768"
FT MUTAGEN 100
FT /note="C->S: Normal growth under denitrifying conditions.
FT Normal growth under aerobic conditions."
FT /evidence="ECO:0000269|PubMed:26787768"
SQ SEQUENCE 211 AA; 22887 MW; A54A11F1964F38C5 CRC64;
MNEGVHGEIH VADPSICRVS EASKETAVES VSRSVGATGD TAAVEFTAPS SAAIDDADEI
FHHEQKTIYR VTHSFTEESR CACELIEGHG CPVRHLEADC GAVVLSFVAA DLESFRDIVV
NLKSAFDGVS LRRLTQSEPD SATGSLVFVD RDELTARQRE VLETAHEMGY FEHPREANAT
EVAAALDINR STFTEHLSAA QSKLLDTILD V
