ID   NART_STAA1              Reviewed;         389 AA.
AC   A7X618;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Probable nitrate transporter NarT;
GN   Name=narT; Synonyms=narK; OrderedLocusNames=SAHV_2372;
OS   Staphylococcus aureus (strain Mu3 / ATCC 700698).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=418127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu3 / ATCC 700698;
RX   PubMed=17954695; DOI=10.1128/aac.00534-07;
RA   Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.;
RT   "Mutated response regulator graR is responsible for phenotypic conversion
RT   of Staphylococcus aureus from heterogeneous vancomycin-intermediate
RT   resistance to vancomycin-intermediate resistance.";
RL   Antimicrob. Agents Chemother. 52:45-53(2008).
CC   -!- FUNCTION: Probably required for nitrate uptake under anoxic conditions.
CC       Also possibly involved in excretion of nitrite produced by the
CC       dissimilatory reduction of nitrate (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Positively regulated by the two-component system NreB/NreC.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR   EMBL; AP009324; BAF79255.1; -; Genomic_DNA.
DR   RefSeq; WP_000278556.1; NC_009782.1.
DR   AlphaFoldDB; A7X618; -.
DR   SMR; A7X618; -.
DR   KEGG; saw:SAHV_2372; -.
DR   HOGENOM; CLU_001265_14_0_9; -.
DR   OMA; DAWWFMF; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   CDD; cd17341; MFS_NRT2_like; 1.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR044772; NO3_transporter.
DR   PANTHER; PTHR23515; PTHR23515; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Nitrate assimilation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..389
FT                   /note="Probable nitrate transporter NarT"
FT                   /id="PRO_0000349392"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        161..181
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        268..288
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        294..314
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        353..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   389 AA;  42175 MW;  9A82DCF0C654D285 CRC64;
     MYKTKGGFQL TLQTLSLVVG FMAWSIIAPL MPFIKQDVNV TEGQISIILA IPVILGSVLR
     VPFGYLTNIV GAKWVFFTSF IVLLFPIFFL SQAQTPGMLM ASGFFLGVGG AIFSVGVTSV
     PKYFPKEKVG LANGIYGMGN IGTAVSSFLA PPIAGIIGWQ TTVRSYLIII ALFALIMFIF
     GDTQERKIKV PLMAQMKTLS KNYKLYYLSY WYFITFGAFV AFGIFLPNYL VNHFGIDKVD
     AGIRSGVFIA LATFLRPIGG ILGDKFNAVK VLMIDFVIMI IGAVILGISD HIALFTVGCL
     TISICAGIGN GLIFKLVPSY FSNEAGSANG IVSMMGGLGG FFPPLVITYV ANLTGSSHLA
     FIFLAVFGCI ALFTMRHLYQ KEYGSLKHS