ID   NART_STAAS              Reviewed;         389 AA.
AC   Q6G6T3;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Probable nitrate transporter NarT;
GN   Name=narT; Synonyms=narK; OrderedLocusNames=SAS2278;
OS   Staphylococcus aureus (strain MSSA476).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=282459;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSSA476;
RX   PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA   Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA   Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA   Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA   Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA   Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA   Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA   Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA   Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT   "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT   for the rapid evolution of virulence and drug resistance.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC   -!- FUNCTION: Probably required for nitrate uptake under anoxic conditions.
CC       Also possibly involved in excretion of nitrite produced by the
CC       dissimilatory reduction of nitrate (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Positively regulated by the two-component system NreB/NreC.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR   EMBL; BX571857; CAG44091.1; -; Genomic_DNA.
DR   RefSeq; WP_000278558.1; NC_002953.3.
DR   AlphaFoldDB; Q6G6T3; -.
DR   SMR; Q6G6T3; -.
DR   KEGG; sas:SAS2278; -.
DR   HOGENOM; CLU_001265_14_0_9; -.
DR   OMA; DAWWFMF; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   CDD; cd17341; MFS_NRT2_like; 1.
DR   Gene3D; 1.20.1250.20; -; 2.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR044772; NO3_transporter.
DR   PANTHER; PTHR23515; PTHR23515; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Nitrate assimilation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..389
FT                   /note="Probable nitrate transporter NarT"
FT                   /id="PRO_0000349391"
FT   TRANSMEM        14..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        161..181
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..231
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        268..288
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        294..314
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        331..351
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        353..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   389 AA;  42148 MW;  A9E89605DAAA3BFF CRC64;
     MYKTKGGFQL TLQTLSLVVG FMAWSIIAPL MPFIKQDVNV TEGQISIILA IPVILGSVLR
     VPFGYLTNIV GAKWVFFTSF IVLLFPIFFL SQAQTPGMLM ASGFFLGVGG AIFSVGVTSV
     PKYFPKEKVG LANGIYGMGN IGTAVSSFLA PPIAGIIGWQ TTVRSYLIII ALFALIMFIF
     GDTQERKIKV PLMAQMKTLS KNYKLYYLSY WYFITFGAFV AFGIFLPNYL VNHFGIDKVD
     AGIRSGVFIA LATFLRPIGG ILGDKFNAVK VLMIDFVVMI IGAIILGISD HIALFTVGCL
     TISICAGIGN GLIFKLVPSY FLNEAGSANG IVSMMGGLGG FFPPLVITYV ANLTGSSHLA
     FIFLAVFGCI ALFTMRHLYQ KEYGSLKNG