NART_STACT
ID NART_STACT Reviewed; 388 AA.
AC O33854; B9DL88;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Probable nitrate transporter NarT;
GN Name=narT; Synonyms=narK; OrderedLocusNames=Sca_1887;
OS Staphylococcus carnosus (strain TM300).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=396513;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION AS A NITRATE TRANSPORTER.
RX PubMed=9082911; DOI=10.1007/bf01682980;
RA Fast B., Lindgren P.-E., Goetz F.;
RT "Cloning, sequencing, and characterization of a gene (narT) encoding a
RT transport protein involved in dissimilatory nitrate reduction in
RT Staphylococcus carnosus.";
RL Arch. Microbiol. 166:361-367(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TM300;
RX PubMed=19060169; DOI=10.1128/aem.01982-08;
RA Rosenstein R., Nerz C., Biswas L., Resch A., Raddatz G., Schuster S.C.,
RA Goetz F.;
RT "Genome analysis of the meat starter culture bacterium Staphylococcus
RT carnosus TM300.";
RL Appl. Environ. Microbiol. 75:811-822(2009).
RN [3]
RP INDUCTION.
RX PubMed=12426351; DOI=10.1128/jb.184.23.6624-6634.2002;
RA Fedtke I., Kamps A., Krismer B., Goetz F.;
RT "The nitrate reductase and nitrite reductase operons and the narT gene of
RT Staphylococcus carnosus are positively controlled by the novel two-
RT component system NreBC.";
RL J. Bacteriol. 184:6624-6634(2002).
CC -!- FUNCTION: Probably required for nitrate uptake under anoxic conditions.
CC Also possibly involved in excretion of nitrite produced by the
CC dissimilatory reduction of nitrate. {ECO:0000269|PubMed:9082911}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Positively regulated by the two-component system NreB/NreC.
CC {ECO:0000269|PubMed:12426351}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; AF029224; AAC45758.1; -; Genomic_DNA.
DR EMBL; AM295250; CAL28793.1; -; Genomic_DNA.
DR RefSeq; WP_015901129.1; NC_012121.1.
DR AlphaFoldDB; O33854; -.
DR SMR; O33854; -.
DR STRING; 396513.SCA_1887; -.
DR TCDB; 2.A.1.8.15; the major facilitator superfamily (mfs).
DR GeneID; 60544399; -.
DR KEGG; sca:SCA_1887; -.
DR eggNOG; COG2223; Bacteria.
DR HOGENOM; CLU_001265_14_0_9; -.
DR OMA; DAWWFMF; -.
DR OrthoDB; 1217595at2; -.
DR BioCyc; SCAR396513:SCA_RS09575-MON; -.
DR Proteomes; UP000000444; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Nitrate assimilation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..388
FT /note="Probable nitrate transporter NarT"
FT /id="PRO_0000349400"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 388 AA; 41871 MW; D495BEE675CD9389 CRC64;
MNKSKGGLQL TVQTLSLVAG FMVWSIIAPL MPMISQDIKI TSSQISIVLA IPVILGSVLR
IPFGYLTNII GAKWVFFSSF IILLFPIFLL SQAQSVNMLM LAGFFLGVGG AVFSVGVTSI
PKYFPKDKVG LANGIYGMGN LGTAVSSFLA PPIAGAIGWQ STVRLYLIVM AVFAIVMFFL
GDAKEPKVKI PLVAQTKDLL KDLRTYYLSF WYFITFGSFV AFGIFLPKYL VDHYELTTVD
AGIRAGIFIA IATFLRPLGG IIGDKIDAVK ALKVDFLFMI IGAIILGIAN DMILFTVGCL
TVSVCAGIGN GLVFKLVPQY FQKEAGVANG IVSMMGGLGG FFPPLVITYV TSITGTSHLA
FIFLALFGVL ALVTMWHLSK KNRSLAYK
