NARU_SALTY
ID NARU_SALTY Reviewed; 462 AA.
AC P37593;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 19-DEC-2001, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Nitrate/nitrite transporter NarU;
DE AltName: Full=Nitrite extrusion protein 2;
DE AltName: Full=Nitrite facilitator 2;
GN Name=narU; OrderedLocusNames=STM1576;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-130.
RC STRAIN=SL1303;
RX PubMed=7926834; DOI=10.1016/0378-1119(94)90255-0;
RA Hongo E., Morimyo M., Mita K., Machida I., Hama-Inaba H., Tsuji H.,
RA Ichimura S., Noda Y.;
RT "The methyl viologen-resistance-encoding gene smvA of Salmonella
RT typhimurium.";
RL Gene 148:173-174(1994).
CC -!- FUNCTION: Catalyzes nitrate uptake, nitrite uptake and nitrite export
CC across the cytoplasmic membrane. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Nitrate/nitrite porter (TC 2.A.1.8) family. {ECO:0000305}.
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DR EMBL; AE006468; AAL20494.1; -; Genomic_DNA.
DR EMBL; D26057; BAA05054.1; -; Genomic_DNA.
DR RefSeq; NP_460535.1; NC_003197.2.
DR RefSeq; WP_000198251.1; NC_003197.2.
DR AlphaFoldDB; P37593; -.
DR SMR; P37593; -.
DR STRING; 99287.STM1576; -.
DR PaxDb; P37593; -.
DR EnsemblBacteria; AAL20494; AAL20494; STM1576.
DR GeneID; 1253094; -.
DR KEGG; stm:STM1576; -.
DR PATRIC; fig|99287.12.peg.1667; -.
DR HOGENOM; CLU_033198_1_0_6; -.
DR OMA; WWYYARR; -.
DR PhylomeDB; P37593; -.
DR BioCyc; SENT99287:STM1576-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015514; F:nitrite efflux transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IBA:GO_Central.
DR GO; GO:0015707; P:nitrite transport; IBA:GO_Central.
DR CDD; cd17341; MFS_NRT2_like; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044772; NO3_transporter.
DR InterPro; IPR004737; NO3_transporter_NarK/NarU-like.
DR PANTHER; PTHR23515; PTHR23515; 1.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00886; 2A0108; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Nitrate assimilation;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..462
FT /note="Nitrate/nitrite transporter NarU"
FT /id="PRO_0000096730"
FT TOPO_DOM 1..35
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..71
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..101
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..124
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..180
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 181..201
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 202..206
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..258
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 280..287
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..317
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..338
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 339..341
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..401
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..422
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 423..432
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 433..453
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 454..462
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 61
FT /note="N -> D (in Ref. 2; BAA05054)"
FT /evidence="ECO:0000305"
FT CONFLICT 126..130
FT /note="TPFGV -> FLLSA (in Ref. 2; BAA05054)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 462 AA; 49751 MW; 2CDC6677B94B0B53 CRC64;
MTRQNENYNR YLLSDWRPEN PAFWENKGKG IARRNLWISV SCLLLAFCVW MLFSAVAVNL
NKIGFNFTTD QLFLLTALPS LSGAILRVPY SFMVPLFGGR KWTVLSTVIL IIPCAWLGFA
VQNPATPFGV FMLIALLCGF AGANFASSMG NISFFFPKAR QGSALGINGG LGNLGVSVMQ
LIAPLVIFLP IFTFLGVQGV PQPDGSLLAL TNAAWIWVPL LAVATLAAWF GMNDIGSSKA
SVASQLPVLK RLHLWLLSLL YLATFGSFIG FSAGFAMLAK TQFPDVNILQ LAFFGPFIGA
LARSAGGVIS DKFGGVRVTL INFIFMALFT ALLFLTLPGS GAGSFSAFYL VFMGLFLTAG
LGSGSTFQMI AVIFRQITLY NVKLRGGSDE QAQREAVTDT AAALGFISAI GAVGGFFIPK
AFGTSLALTG SPVGAMKIFL LFYLACVLLT WLVYGRRKPK QQ
