ID   NARW_ECOLI              Reviewed;         231 AA.
AC   P19317;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 150.
DE   RecName: Full=Probable nitrate reductase molybdenum cofactor assembly chaperone NarW;
DE   AltName: Full=Redox enzyme maturation protein NarW;
GN   Name=narW; OrderedLocusNames=b1466, JW1461;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2233673; DOI=10.1007/bf00283030;
RA   Blasco F., Iobbi C., Ratouchniak J., Bonnefoy V., Chippaux M.;
RT   "Nitrate reductases of Escherichia coli: sequence of the second nitrate
RT   reductase and comparison with that encoded by the narGHJI operon.";
RL   Mol. Gen. Genet. 222:104-111(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA   Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA   Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA   Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA   Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA   Wada C., Yamamoto Y., Horiuchi T.;
RT   "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 28.0-40.1 min region on the linkage map.";
RL   DNA Res. 3:363-377(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Chaperone required for proper molybdenum cofactor insertion
CC       and final assembly of the membrane-bound respiratory nitrate reductase
CC       2. {ECO:0000250}.
CC   -!- INTERACTION:
CC       P19317; P09152: narG; NbExp=9; IntAct=EBI-555088, EBI-547248;
CC       P19317; P11349: narH; NbExp=3; IntAct=EBI-555088, EBI-555067;
CC       P19317; P19318: narY; NbExp=4; IntAct=EBI-555088, EBI-555059;
CC       P19317; P19319: narZ; NbExp=3; IntAct=EBI-555088, EBI-547262;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NarJ/NarW family. {ECO:0000305}.
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DR   EMBL; X17110; CAA34966.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC74548.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA15103.1; -; Genomic_DNA.
DR   PIR; S11429; S11429.
DR   RefSeq; NP_415983.1; NC_000913.3.
DR   RefSeq; WP_001166353.1; NZ_STEB01000053.1.
DR   AlphaFoldDB; P19317; -.
DR   SMR; P19317; -.
DR   BioGRID; 4260181; 191.
DR   BioGRID; 850393; 7.
DR   DIP; DIP-10321N; -.
DR   IntAct; P19317; 8.
DR   STRING; 511145.b1466; -.
DR   PaxDb; P19317; -.
DR   PRIDE; P19317; -.
DR   EnsemblBacteria; AAC74548; AAC74548; b1466.
DR   EnsemblBacteria; BAA15103; BAA15103; BAA15103.
DR   GeneID; 946032; -.
DR   KEGG; ecj:JW1461; -.
DR   KEGG; eco:b1466; -.
DR   PATRIC; fig|1411691.4.peg.802; -.
DR   EchoBASE; EB0639; -.
DR   eggNOG; COG2180; Bacteria.
DR   HOGENOM; CLU_084469_0_0_6; -.
DR   InParanoid; P19317; -.
DR   OMA; YGDTRKR; -.
DR   PhylomeDB; P19317; -.
DR   BioCyc; EcoCyc:NARW-MON; -.
DR   PRO; PR:P19317; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016530; F:metallochaperone activity; IBA:GO_Central.
DR   GO; GO:0051082; F:unfolded protein binding; IEA:InterPro.
DR   GO; GO:0051131; P:chaperone-mediated protein complex assembly; IBA:GO_Central.
DR   GO; GO:0042128; P:nitrate assimilation; IBA:GO_Central.
DR   InterPro; IPR020945; DMSO/NO3_reduct_chaperone.
DR   InterPro; IPR003765; NO3_reductase_chaperone_NarJ.
DR   InterPro; IPR036411; TorD-like_sf.
DR   PANTHER; PTHR43680; PTHR43680; 1.
DR   Pfam; PF02613; Nitrate_red_del; 1.
DR   SUPFAM; SSF89155; SSF89155; 1.
DR   TIGRFAMs; TIGR00684; narJ; 1.
PE   1: Evidence at protein level;
KW   Chaperone; Cytoplasm; Nitrate assimilation; Reference proteome.
FT   CHAIN           1..231
FT                   /note="Probable nitrate reductase molybdenum cofactor
FT                   assembly chaperone NarW"
FT                   /id="PRO_0000096733"
SQ   SEQUENCE   231 AA;  26161 MW;  30AE8EC9AE6290AB CRC64;
     MQILKVIGLL MEYPDELLWE CKEDALALIR RDAPMLTDFT HNLLNAPLLD KQAEWCEVFD
     RGRTTSLLLF EHVHAESRDR GQAMVDLLAE YEKVGLQLDC RELPDYLPLY LEYLSVLPDD
     QAKEGLLNVA PILALLGGRL KQREAPWYAL FDALLQLAGS SLSSDSVTKQ VNSEERDDTR
     QALDAVWEEE QVKFIEDNAT ACDSSPLNQY QRRFSQDVAP QYVDISAGGG K