NAS1_ARATH
ID NAS1_ARATH Reviewed; 320 AA.
AC Q9FF79; Q9ZWF8;
DT 13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Nicotianamine synthase 1;
DE EC=2.5.1.43;
DE AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 1;
DE Short=AtNAS1;
GN Name=NAS1; OrderedLocusNames=At5g04950; ORFNames=MUG13.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Suzuki K., Higuchi K., Nakanishi H., Nishizawa N.-K., Mori S.;
RT "Cloning of nicotianamine synthase genes from Arabidopsis thaliana.";
RL Soil Sci. Plant Nutr. 45:993-1002(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT features of the 1.6 Mb regions covered by twenty physically assigned P1
RT clones.";
RL DNA Res. 4:215-230(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Synthesizes nicotianamine, a polyamine which serves as a
CC sensor for the physiological iron status within the plant, and/or might
CC be involved in the transport of iron.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC -!- TISSUE SPECIFICITY: In shoots and roots.
CC -!- INDUCTION: Constitutively expressed.
CC -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC {ECO:0000305}.
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DR EMBL; AB021934; BAA74589.1; -; Genomic_DNA.
DR EMBL; AB005245; BAB11517.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90808.1; -; Genomic_DNA.
DR EMBL; AY072364; AAL62356.1; -; mRNA.
DR EMBL; BT000392; AAN15711.1; -; mRNA.
DR RefSeq; NP_196114.1; NM_120577.4.
DR AlphaFoldDB; Q9FF79; -.
DR SMR; Q9FF79; -.
DR STRING; 3702.AT5G04950.1; -.
DR PaxDb; Q9FF79; -.
DR PRIDE; Q9FF79; -.
DR ProteomicsDB; 251090; -.
DR DNASU; 830377; -.
DR EnsemblPlants; AT5G04950.1; AT5G04950.1; AT5G04950.
DR GeneID; 830377; -.
DR Gramene; AT5G04950.1; AT5G04950.1; AT5G04950.
DR KEGG; ath:AT5G04950; -.
DR Araport; AT5G04950; -.
DR TAIR; locus:2175304; AT5G04950.
DR eggNOG; ENOG502QTU6; Eukaryota.
DR HOGENOM; CLU_031919_1_1_1; -.
DR InParanoid; Q9FF79; -.
DR OMA; IHAIMNN; -.
DR OrthoDB; 1009301at2759; -.
DR PhylomeDB; Q9FF79; -.
DR BioCyc; ARA:AT5G04950-MON; -.
DR BRENDA; 2.5.1.43; 399.
DR PRO; PR:Q9FF79; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FF79; baseline and differential.
DR Genevisible; Q9FF79; AT.
DR GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR GO; GO:0010233; P:phloem transport; IGI:TAIR.
DR GO; GO:0009555; P:pollen development; IGI:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IGI:TAIR.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR004298; Nicotian_synth.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR32266; PTHR32266; 1.
DR Pfam; PF03059; NAS; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51142; NAS; 1.
PE 2: Evidence at transcript level;
KW Reference proteome; S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..320
FT /note="Nicotianamine synthase 1"
FT /id="PRO_0000212700"
FT CONFLICT 193
FT /note="G -> A (in Ref. 1; BAA74589)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="S -> R (in Ref. 1; BAA74589)"
FT /evidence="ECO:0000305"
FT CONFLICT 318
FT /note="A -> T (in Ref. 1; BAA74589)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 320 AA; 35547 MW; 201096BFDF0FCFA0 CRC64;
MACQNNLVVK QIIDLYDQIS KLKSLKPSKN VDTLFGQLVS TCLPTDTNID VTNMCEEVKD
MRANLIKLCG EAEGYLEQHF STILGSLQED QNPLDHLHIF PYYSNYLKLG KLEFDLLSQH
SSHVPTKIAF VGSGPMPLTS IVLAKFHLPN TTFHNFDIDS HANTLASNLV SRDPDLSKRM
IFHTTDVLNA TEGLDQYDVV FLAALVGMDK ESKVKAIEHL EKHMAPGAVL MLRSAHALRA
FLYPIVDSSD LKGFQLLTIY HPTDDVVNSV VIARKLGGPT TPGVNGTRGC MFMPCNCSKI
HAIMNNRGKK NMIEEFSAIE
