ID   NAS1_HORVU              Reviewed;         328 AA.
AC   Q9ZQV9;
DT   13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Nicotianamine synthase 1;
DE            EC=2.5.1.43;
DE   AltName: Full=HvNAS1;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 1;
GN   Name=NAS1;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 2-20; 64-70; 182-244
RP   AND 248-267.
RC   STRAIN=cv. Ehimehadaka No.1; TISSUE=Root;
RX   PubMed=9952442; DOI=10.1104/pp.119.2.471;
RA   Higuchi K., Suzuki K., Nakanishi H., Yamaguchi H., Nishizawa N.-K.,
RA   Mori S.;
RT   "Cloning of nicotianamine synthase genes, novel genes involved in the
RT   biosynthesis of phytosiderophores.";
RL   Plant Physiol. 119:471-480(1999).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serves as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- TISSUE SPECIFICITY: In roots but not in leaves.
CC   -!- INDUCTION: By iron deficiency.
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
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DR   EMBL; AB010086; BAA74580.1; -; mRNA.
DR   AlphaFoldDB; Q9ZQV9; -.
DR   SMR; Q9ZQV9; -.
DR   BioCyc; MetaCyc:MON-13940; -.
DR   BRENDA; 2.5.1.43; 2687.
DR   ExpressionAtlas; Q9ZQV9; baseline.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 2.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; S-adenosyl-L-methionine; Transferase.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:9952442"
FT   CHAIN           2..328
FT                   /note="Nicotianamine synthase 1"
FT                   /id="PRO_0000212704"
SQ   SEQUENCE   328 AA;  35146 MW;  47AB32B8F75399A7 CRC64;
     MDAQNKEVAA LIEKIAGIQA AIAELPSLSP SPEVDRLFTD LVTACVPPSP VDVTKLSPEH
     QRMREALIRL CSAAEGKLEA HYADLLATFD NPLDHLGLFP YYSNYVNLSR LEYELLARHV
     PGIAPARVAF VGSGPLPFSS LVLAAHHLPE TQFDNYDLCG AANERARKLF GATADGVGAR
     MSFHTADVAD LTQELGAYDV VFLAALVGMA AEEKAKVIAH LGAHMVEGAS LVVRSARPRG
     FLYPIVDPED IRRGGFEVLA VHHPEGEVIN SVIVARKAVE AQLSGPQNGD AHARGAVPLV
     SPPCNFSTKM EASALEKSEE LTAKELAF