ID   NAS1_ORYSJ              Reviewed;         332 AA.
AC   Q0DSH9; Q9SXQ7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Nicotianamine synthase 1;
DE            EC=2.5.1.43;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 1;
DE            Short=OsNAS1;
GN   Name=NAS1; OrderedLocusNames=Os03g0307300, LOC_Os03g19427;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=14617093; DOI=10.1046/j.1365-313x.2003.01878.x;
RA   Inoue H., Higuchi K., Takahashi M., Nakanishi H., Mori S., Nishizawa N.K.;
RT   "Three rice nicotianamine synthase genes, OsNAS1, OsNAS2, and OsNAS3 are
RT   expressed in cells involved in long-distance transport of iron and
RT   differentially regulated by iron.";
RL   Plant J. 36:366-381(2003).
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine that is the first
CC       intermediate in the synthesis of the phytosiderophores of the mugineic
CC       acid type found in gramineae which serve as a sensor for the
CC       physiological iron status within the plant, and/or might be involved in
CC       the transport of iron. {ECO:0000269|PubMed:14617093}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- TISSUE SPECIFICITY: Expressed in roots. {ECO:0000269|PubMed:14617093}.
CC   -!- INDUCTION: By iron deficiency in roots and chlorotic leaves.
CC       {ECO:0000269|PubMed:14617093}.
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
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DR   EMBL; AP008209; BAF11809.1; -; Genomic_DNA.
DR   EMBL; AP014959; BAS83822.1; -; Genomic_DNA.
DR   EMBL; AK112069; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015630629.1; XM_015775143.1.
DR   AlphaFoldDB; Q0DSH9; -.
DR   SMR; Q0DSH9; -.
DR   STRING; 4530.OS03T0307300-01; -.
DR   PaxDb; Q0DSH9; -.
DR   PRIDE; Q0DSH9; -.
DR   EnsemblPlants; Os03t0307300-01; Os03t0307300-01; Os03g0307300.
DR   GeneID; 4332608; -.
DR   Gramene; Os03t0307300-01; Os03t0307300-01; Os03g0307300.
DR   KEGG; osa:4332608; -.
DR   eggNOG; ENOG502QTU6; Eukaryota.
DR   HOGENOM; CLU_031919_0_0_1; -.
DR   InParanoid; Q0DSH9; -.
DR   OMA; STCCKVE; -.
DR   OrthoDB; 1009301at2759; -.
DR   BRENDA; 2.5.1.43; 4460.
DR   Proteomes; UP000000763; Chromosome 3.
DR   Proteomes; UP000059680; Chromosome 3.
DR   ExpressionAtlas; Q0DSH9; baseline and differential.
DR   Genevisible; Q0DSH9; OS.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 2.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..332
FT                   /note="Nicotianamine synthase 1"
FT                   /id="PRO_0000212714"
SQ   SEQUENCE   332 AA;  34933 MW;  F6A8115693D60BF3 CRC64;
     MEAQNQEVAA LVEKIAGLHA AISKLPSLSP SAEVDALFTD LVTACVPASP VDVAKLGPEA
     QAMREELIRL CSAAEGHLEA HYADMLAAFD NPLDHLARFP YYGNYVNLSK LEYDLLVRYV
     PGIAPTRVAF VGSGPLPFSS LVLAAHHLPD AVFDNYDRCG AANERARRLF RGADEGLGAR
     MAFHTADVAT LTGELGAYDV VFLAALVGMA AEEKAGVIAH LGAHMADGAA LVVRSAHGAR
     GFLYPIVDPE DVRRGGFDVL AVCHPEDEVI NSVIVARKVG AAAAAAAARR DELADSRGVV
     LPVVGPPSTC CKVEASAVEK AEEFAANKEL SV