ID   NAS2_ARATH              Reviewed;         320 AA.
AC   Q9FKT9; A2RVM2; Q9ZWF7;
DT   13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Nicotianamine synthase 2;
DE            EC=2.5.1.43;
DE   AltName: Full=S-adenosyl-L-methionine:S-adenosyl-L-methionine:S-adenosyl-methionine 3-amino-3-carboxypropyltransferase 2;
DE            Short=AtNAS2;
GN   Name=NAS2; OrderedLocusNames=At5g56080; ORFNames=MDA7.14;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RA   Suzuki K., Higuchi K., Nakanishi H., Nishizawa N.-K., Mori S.;
RT   "Cloning of nicotianamine synthase genes from Arabidopsis thaliana.";
RL   Soil Sci. Plant Nutr. 45:993-1002(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA   Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT   features of the regions of 1,381,565 bp covered by twenty one physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:131-145(1998).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Synthesizes nicotianamine, a polyamine which serves as a
CC       sensor for the physiological iron status within the plant, and/or might
CC       be involved in the transport of iron.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 S-adenosyl-L-methionine = 3 H(+) + nicotianamine + 3 S-
CC         methyl-5'-thioadenosine; Xref=Rhea:RHEA:16481, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17509, ChEBI:CHEBI:58249, ChEBI:CHEBI:59789; EC=2.5.1.43;
CC   -!- SIMILARITY: Belongs to the nicotianamine synthase (NAS)-like family.
CC       {ECO:0000305}.
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DR   EMBL; AB021935; BAA74590.1; -; Genomic_DNA.
DR   EMBL; AB011476; BAB09290.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96719.1; -; Genomic_DNA.
DR   EMBL; BT030013; ABN04751.1; -; mRNA.
DR   RefSeq; NP_200419.1; NM_124990.2.
DR   AlphaFoldDB; Q9FKT9; -.
DR   SMR; Q9FKT9; -.
DR   STRING; 3702.AT5G56080.1; -.
DR   PaxDb; Q9FKT9; -.
DR   PRIDE; Q9FKT9; -.
DR   ProteomicsDB; 251038; -.
DR   EnsemblPlants; AT5G56080.1; AT5G56080.1; AT5G56080.
DR   GeneID; 835707; -.
DR   Gramene; AT5G56080.1; AT5G56080.1; AT5G56080.
DR   KEGG; ath:AT5G56080; -.
DR   Araport; AT5G56080; -.
DR   TAIR; locus:2161770; AT5G56080.
DR   eggNOG; ENOG502QTU6; Eukaryota.
DR   HOGENOM; CLU_031919_1_1_1; -.
DR   InParanoid; Q9FKT9; -.
DR   OMA; STCCKVE; -.
DR   OrthoDB; 1009301at2759; -.
DR   PhylomeDB; Q9FKT9; -.
DR   BioCyc; ARA:AT5G56080-MON; -.
DR   BRENDA; 2.5.1.43; 399.
DR   PRO; PR:Q9FKT9; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FKT9; baseline and differential.
DR   Genevisible; Q9FKT9; AT.
DR   GO; GO:0030410; F:nicotianamine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030418; P:nicotianamine biosynthetic process; IEA:InterPro.
DR   GO; GO:0010233; P:phloem transport; IGI:TAIR.
DR   GO; GO:0009555; P:pollen development; IGI:TAIR.
DR   GO; GO:0009860; P:pollen tube growth; IGI:TAIR.
DR   GO; GO:0010043; P:response to zinc ion; IEP:TAIR.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR004298; Nicotian_synth.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR32266; PTHR32266; 1.
DR   Pfam; PF03059; NAS; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51142; NAS; 1.
PE   2: Evidence at transcript level;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..320
FT                   /note="Nicotianamine synthase 2"
FT                   /id="PRO_0000212701"
FT   CONFLICT        4
FT                   /note="E -> Q (in Ref. 1; BAA74590)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        316
FT                   /note="Y -> F (in Ref. 1; BAA74590)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   320 AA;  35679 MW;  0F089819D3C05FCA CRC64;
     MACENNLVVK QIMDLYNQIS NLESLKPSKN VDTLFRQLVS TCLPTDTNID VTEIHDEKVK
     DMRSHLIKLC GEAEGYLEQH FSAILGSFED NPLNHLHIFP YYNNYLKLGK LEFDLLSQHT
     THVPTKVAFI GSGPMPLTSI VLAKFHLPNT TFHNFDIDSH ANTLASNLVS RDSDLSKRMI
     FHTTDVLNAK EGLDQYDVVF LAALVGMDKE SKVKAIEHLE KHMAPGAVVM LRSAHGLRAF
     LYPIVDSCDL KGFEVLTIYH PSDDVVNSVV IARKLGGSNG ARGSQIGRCV VMPCNCSKVH
     AILNNRGMEK NLIEEYSAIE